PurposeThis study investigated the effect of three digital bite registration techniques on the accuracy of intraoral scanning in maxillary edentulous and mandibular dentate arches.Materials and MethodsMaxillary edentulous and mandible fully dentate models were created. Four dental implants were placed in the maxilla, fitted with scan bodies, and reference scans were obtained using a Nikon Altera 10.7.6 scanner. Digital impressions were acquired 10 times for each model using a Trios 4 intraoral scanner. Three different digital bite records were collected for each pair of mandible and maxilla scans. The first one was a bite record without additional material (WSB), the second utilized a silicone index between the models (WSB silicone), and the third followed a pre‐preparation scanning protocol (Pre‐PREP). The data was exported in standard tessellation language (STL) format, which was assessed for trueness and precision using statistical analysis.ResultsAccording to mean trueness values, WSB exhibited the lowest value, 173 µm, followed by WSB silicone 242 µm and Pre‐PREP 620 µm. The differences were significant only when comparing Pre‐PREP to WSB and Pre‐PREP to WSB silicone (p < 0.05). Regarding precision, WSB demonstrated a mean value of 171.5 µm, followed by WSB silicone with 213.8 µm, and Pre‐PREP with 222.2 µm with no significant difference between the groups.ConclusionThe presence of interarch bite registration material adversely affected interocclusal record accuracy, while WSB silicone presented a suitable substitute for WSB.