Effect of different levels of n-3 polyunsaturated fatty acids rich fish oil supplementation on the ovarian and endometrial functions in the goat (Capra hircus)

Reprod Domestic Animals

et al. 2020

Self Cite

Emerging evidence indicates that dietary n‐3 polyunsaturated fatty acids (PUFA) alter the fatty acid composition of corpus luteum (CL) and directly affect the luteal function in the cow, which is independent of the inhibitory effect on the endometrial PGF2α production. The present study, thus, investigated the effects of n‐3 PUFA rich fish oil (FO) supplementation on the transcriptional modulation of genes involved in the biosynthesis of progesterone (P4) in the CL collected during the luteolytic phase of oestrous cycle in the goat. On the day of synchronized oestrus, goats (n = 6/group) were fed an isocaloric diet supplemented with either FO or palm oil (PO). The dose of oil supplementation was 0.6 mlkg‐1 body weight, and the duration was 55–57 days. The FO provided 156 mgkg‐1 body weight of n‐3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The CL was collected by laparotomy on day 16 post‐oestrus, and the relative abundance of P450 side‐chain cleaving enzyme, steroid acute regulatory protein (StAR) and 3β‐hydroxy steroid dehydrogenase (3β‐HSD) genes was quantitated by real‐time PCR. The results indicated that the dietary FO significantly upregulated the expression of 3β‐HSD by 1.13‐fold and downregulated StAR by ~2‐fold as compared to PO group (p < .05). It is concluded that dietary FO differently affected the expression of genes involved in P4 synthesis in the CL during the luteolytic window of the oestrous cycle in the goat.

Section: Methodsmentioning

confidence: 99%

Effect of dietary n‐3 polyunsaturated fatty acid supplementation on the expression of genes involved in progesterone biosynthesis in the corpus luteum of goat (Capra hircus)

Chaudhari

Mahla

Reprod Domestic Animals

et al. 2020

Self Cite

“…Goats in the treatment group were fed concentrate feed supplemented with n‐3 PUFA‐rich refined FO (Avestia Pharma) containing 16.4% EPA and 10.2% DHA (total n‐3 PUFA 26.6%) on body weight basis. A dose of 156 mg/kg EPA and DHA was chosen based on our earlier studies in non‐pregnant does (Mahla et al, and Verma et al, ). Palm oil (PO; Raag ® , Adani Wilmar Limited) was offered to goats in the control group according to the body weight to make the diet isocaloric.…”

Section: Methodsmentioning

confidence: 99%

“…We reported that fish oil (FO) containing 16.4% EPA and 10.2% DHA (total n‐3 PUFA 26.6%) at the rate of 156 mg/kg (0.6 ml/kg body weight/day/doe) for 71 days around mating inhibited endometrial PGF 2α production following oxytocin (OXT) challenge, and improved the CL growth and blood progesterone (P 4 ) concentration in non‐pregnant goat (Verma et al, ). Further, supplementation of FO for 10 weeks during the breeding season increased the number of preovulatory follicles, improved the ovulation rate by 41.4% and favoured embryo survival by inhibiting the PGF 2α during the window of maternal recognition of pregnancy (Mahla et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effect of n‐3 PUFA‐rich fish oil supplementation during late gestation on kidding, uterine involution and resumption of follicular activity in goat

Yadav

Reprod Domestic Animals

Kumar

et al. 2019

Self Cite

We have shown that dietary supplementation of n‐3 polyunsaturated fatty acid (n‐3 PUFA)‐rich fish oil (FO) around the breeding time improved the utero‐ovarian functions in the goat. Here, we investigated the effect of FO supplementation during the periparturient period on serum n‐3 PUFA, prostaglandin F2α metabolite (PGFM), placental expulsion, uterine involution, resumption of oestrus and neonatal vigour. Rohilkhandi goat in advanced gestation (n = 16) was divided into two equal groups. One group was supplemented with FO containing 26% n‐3 long‐chain PUFA at the rate of 156 mg per kg body weight, while the control group was fed isocaloric palm oil (PO) from −3 to +3 week of kidding. Dietary FO increased serum concentration of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) by 7.3‐ and 6.6‐fold, respectively, after 6 weeks of supplementation. Goats in FO group expelled the foetal membranes 99.1 min earlier (p < .01) than those of PO group. Further, dietary FO significantly decreased the serum PGFM on day 7 post‐partum. However, no difference was found on uterine involution, which was complete by day 20 post‐partum in either group. Resumption of follicular activity by day 5 post‐partum was 87.5% in the FO as compared to 25% in the PO group (p < .05). Similarly, occurrence of behavioural oestrus by day 90 post‐partum was 57.1% in goats of the FO group while none of does was in the PO group (p < .01) expressed oestrus. It was concluded that feeding FO‐rich diet during −3 to +3 weeks of kidding decreased the PGFM till day 7 post‐partum, hastened the expulsion of foetal membranes and reduced the time from kidding to first post‐partum oestrus in Rohilkhandi does.

“…Goats in the treatment group were fed concentrate feed supplemented with n-3 PUFA rich refined FO (Avestia Pharma, Mumbai). A dose of 156 mg/kg was chosen (Mahla et al 2017, Verma et al 2018. Palm oil (PO) was supplemented to the goats of control group according to the body weight to make the diet isocaloric.…”

Section: Experimental Animals and Supplementation Schedulementioning

confidence: 99%

“…In goats, dietary EPA and DHA rich FO @ 72 mg/kg body weight for 8 weeks inhibited oxytocin induced PGF 2α pulses and estradiol (E 2 ) during the luteolytic window of estrous cycle (Verma et al 2018). Further, supplementation of FO @ 156 mg/kg body weight for 10 weeks during the breeding season increased twinning by 25% and improved embryo survival by decreasing the PGFM and E 2 concentration during the window of maternal recognition of pregnancy (Mahla et al 2016(Mahla et al , 2017 through the down regulation of COX-2, cPLA2 and PGFS transcripts (Chaudhari et al 2018).…”

mentioning

confidence: 99%

Effect of n-3 PUFA rich fish oil supplementation on the reproductive performance of seasonally acyclic goats

Yadav

Teeli

et al. 2019

Indian J of Anim Sci

Self Cite

In the present study, effect of dietary supplementation of fish oil during the non-breeding season on the reproductive performance of the goats was investigated. Experimental does were fed an isocaloric diet of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) rich fish (FO; n=12) or palm oil (PO; n=12) for 71 days. Periodical ovarian scanning for 21 days from the day of supplementation (day 0) confirmed acyclicity. Estrus induction was done by intra-vaginal progesterone (P4) sponge for 14 days from day 22 to 35 of supplementation. Dietary FO did not affect serum P4 throughout the period of supplementation. Ovarian scanning studies revealed that neither the number of surface follicles nor the diameter of largest surface follicle was significantly different between the groups at any point of supplementation. Similarly, the concentration of serum estradiol (E2) and P4 on the day of induced estrus was comparable between the groups. However, supplementation of FO decreased the PGFM significantly on day 16â€“18 post-estrus. In conclusion, supplementation of EPA and DHA rich FO for about 10 weeks inhibited the endometrial PGF2Î± production during the luteolytic window at induced estrus; however, it did not improve the ovarian function and fertility in the seasonally acyclic goat.