Effect of Different Light and Two Polysaccharides on the Proliferation of Protocorm-Like Bodies of Cymbidium Cultured in Vitro

Nahar, Syeda Jabun; Shimasaki, Kazuhiko; Haque, Syed Mostafizul

doi:10.17660/actahortic.2012.956.35

Cited by 15 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results were supported with previous data on C. insigne (Nahar et al, 2012), whereas the average number of PLBs, maximum formation of PLB and fresh weight of PLBs were higher under green LED, and shoot formation increased under white fluorescent tube. Sultana et al (2014) reported that blue and red LED plays an important role on organogenesis of Dendrobium kingianum, while sucrose was used as a carbon sources with medium.…”

Section: In Vitro Culture Of Plbs In Modified Ms Medium Supplemented supporting

confidence: 92%

“…They are particularly well suited for research applications (e.g., in growth chambers) as a result of their unique capabilities and lend themselves well to shelf lighting for tissue culture applications as a result of their low profile and low radiant heat output (Bula et al, 1991;Barta et al, 1992;Morrow, 2008). The effects of LEDs on plantlets cultured in vitro were reported in many plants such as potato (Miyashita et al, 1995), Cymbidium (Tanaka et al, 1998;Nahar et al, 2012Nahar et al, , 2013Kaewjampa and Shimasaki, 2012), Dendrobium (Sultana et al, 2014), Rehmannia glutinose (Hahn et al, 2000) and strawberry (Nhut et al, 2003). The reaction to different lighting conditions differs between species and their various growth stages.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Effect of Light Quality, Sucrose and Trehalose on In Vitro Organogenesis of Cymbidium devonianum (Lindl.)

2017

View full text Add to dashboard Cite

The present study investigated the effect of sucrose, trehalose and combination of sucrose and trehalose with modified MS medium on in vitro regulation of protocorm-like bodies (PLBs) of Cymbidium devonianum under different quality of lights (white fluorescent tube, green, red and blue LED). As a result of this study, new PLB and shoots were successfully regenerated on modified MS medium under different quality of lights. The highest PLB formation rate (100%) and the highest shoot formation rate (85%) were observed amongst explants cultured on medium supplemented with 10 g/l sucrose + 10 g/l trehalose under green LED. The maximum fresh weight of PLBs, the highest average number of PLBs and shoots were recorded on medium containing 10 g/l sucrose + 10 g/l trehalose under green LED. For plant tissue culture, sucrose is considered an indisputably important carbon and energy source and biosynthesis of trehalose is similar to that of sucrose. The hereby study concluded that the contribution of LED lights, sucrose and trehalose (combined) can induce PLB and shoot formation of Cymbidium devonianum tissue culture without the use of any other plant growth regulator, whereas the green light showed the best formation rate compare with the other studied qualities of lights.

show abstract

Section: In Vitro Culture Of Plbs In Modified Ms Medium Supplemented supporting

confidence: 92%

Section: Introductionmentioning

confidence: 98%

Effect of Light Quality, Sucrose and Trehalose on In Vitro Organogenesis of Cymbidium devonianum (Lindl.)

2017

View full text Add to dashboard Cite

show abstract

“…The maximum number of PLBs (18.7 PLBs/explant) was observed at 0.1 mg/l chondroitin sulfate under red LED, while the minimum number of PLBs (1.7 PLBs/explant) was observed under white fluorescent tube at control. These results were supported with previous data on C. insigne (Nahar et al, 2012), whereas it was observed that chondroitin sulphate, at every concentration tested, enhanced the growth of PLBs and shoots formation when compared to control treatment; even green LED showed best formation of PLBs and shoots. Xu et al (2009) reported that PLBs cultured under red LEDs showed the lowest differentiation rate, compared to blue LEDs, which resulted in the highest differentiation rate in cultures of Oncidium cultured in vitro.…”

Section: In Vitro Growth Of Plbs In Modified Ms Medium Supplemented With Chondroitin Sulphate Under Different Sources Of Lightssupporting

confidence: 91%

“…The efficiency of the elicitation process depends on the plant material, contact time and elicitor concentration. The simultaneous use of elicitation by chondroitin sulfate and different sources of light irradiation in Cymbidium insigne PLBs cultures resulted in a significant synergistic enhancement of PLBs, shoots and roots accumulation, showing the potential of integrated processes on chondroitin sulfate accumulation under in vitro conditions (Nahar et al, 2012).…”

Section: Introductionmentioning

confidence: 92%

Application of Chondroitin Sulfate on Organogenesis of Two <i>Cymbidium</i> spp. under Different Sources of Lights

2016

View full text Add to dashboard Cite

The aim of this study was to present chondroitin sulfate as a plant growth regulator and to give an overview about light effects on PLBs (protocorm like bodies) culture of Cymbidium dayanum and Cymbidium finlaysonianum cultured in vitro. Chondroitin sulfate is a sulfated glycosaminoglycan (GAG) composed of a chain of alternating sugars N-acetylgalactosamine and glucuronic acid. It is widely used as a material for food ingredients, cosmetics and medicine. PLBs were cultured on modified MS medium containing different concentration of chondroitin sulfate (0, 0.1, 1 and 10 mg/l), under four sources of lights: conventional white fluorescent tube, red LED, green LED and blue LED. In C. dayanum, 100% PLBs formation rate was observed at 0.1 mg/l chondroitin sulfate with modified MS medium under green LED and 1 mg/l chondroitin sulfate under blue LED; the maximum shoots and roots formation were observed under green LEDs (93% and 80% respectively) when media contained 0.1 mg/l chondroitin sulfate. In C. finlaysonianum, every concentrations of chondroitin sulfate enhanced the growth rate of PLBs when compared to control treatment, under all four sources of lights. The highest values were recorded with 0.1 mg/l chondroitin sulfate which induced 100% PLBs formation under blue LED, while 10 mg/l chondroitin sulfate had induced 100% PLBs formation under green LED. The highest percentage of shoots (73%) was initiated in the medium containing 10 mg/l chondroitin sulfate under green LED. Plant development was strongly influenced by the light quality and plant growth regulator functions as chemical messengers for intercellular communication of plant. The results demonstrated that low concentrations of chondroitin sulfate could promote PLBs, shoots and roots formation of Cymbidium spp. under green and blue LED.

show abstract

“…Some studies were also reported about the effects of white, green, far red, yellow and orange LEDs in plant micropropagation [25][26][27][28][29][30]. LEDs are found more efficient over fluorescent light for orchid PLB organogenesis [6,7,[31][32][33][34][35][36][37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Low Concentration of Putative Anti-Auxin and Anti-Fungal Agent Accelerates the PLB Organogenesis of <em>Dendrobium okinawense</em> Under Green LED

Mehbub¹,

Shimasaki²,

Mehraj³

2021

Preprint

View full text Add to dashboard Cite

Dendrobium okinawense is an endangered epiphytic orchid, and there has been no scientific report so far on its propagation. Protocorm is mass of cell, and protocorm-like bodies (PLBs) look alike protocorm produced by vegetative explants in vitro. Regeneration of PLBs is the most efficient technique for the orchids micro-propagation. We used different light emitting diodes (LEDs) for the efficient PLB organogenesis of D. okinawense. PLBs regenartion under green and red LED surpassed respectively 81.1% and 71.6% in numbers, and respectively 80.8% and 57.8% in fresh weight over white fluorescent light. We manipulated the culture media by different concentrations of PCIB and HMI. PLBs organogenesis promoted by low concentration, it increased respectively 35.9% and 19.3% over control by 0.01 mg/L PCIB and 0.01 ml/L HMI in numbers. Green LED and PCIB independently produced mostly similar numbers of new PLBs. Interestingly, culture media with 0.01 mg/L of PCIB further increased 8.5% of the numbers of PLBs under green LED, whereas the culture media with 0.01 mg/L HMI reduced the number of PLBs under green LED. PLBs culture under green LED with very low concentrations of PCIB manipulated culture media can significantly increase their organogenesis of D. okinawense.

show abstract

Effect of Different Light and Two Polysaccharides on the Proliferation of Protocorm-Like Bodies of Cymbidium Cultured in Vitro

Cited by 15 publications

References 0 publications

Effect of Light Quality, Sucrose and Trehalose on In Vitro Organogenesis of Cymbidium devonianum (Lindl.)

Effect of Light Quality, Sucrose and Trehalose on In Vitro Organogenesis of Cymbidium devonianum (Lindl.)

Application of Chondroitin Sulfate on Organogenesis of Two <i>Cymbidium</i> spp. under Different Sources of Lights

Low Concentration of Putative Anti-Auxin and Anti-Fungal Agent Accelerates the PLB Organogenesis of <em>Dendrobium okinawense</em> Under Green LED

Contact Info

Product

Resources

About