Effect of different liver resection methods on liver damage and regeneration factors VEGF and FGF-2 in mice

Abstract

Background: Different approaches to study liver regeneration in murine models have been proposed. We investigated the effect of different liver resection models on liver damage and regeneration parameters in mice. Methods:We compared the technical aspect of the 2 most commonly used techniques of 50% and 70% liver resection. Liver damage, as determined by the change in serum alanine aminotransferase and aspartate aminotransferase, as well as the regeneration parameters VEGF and FGF-2 were analyzed at 6 time points. A postoperative vitality score was introduced.Results: Cholestasis was not observed for either technique. Both resection techniques resulted in full weight recovery of the liver after 240 hours, with no significant difference between sham and resection groups. Postoperative animal morbidity and total protein levels did not differ significantly for either method, indicating early and full functional recovery. However, comparing the mitogenic growth factors FGF-2 and VEGF, a significant increase in serum levels and, therefore, increased growth stimulus, was shown in the extended resection group. Conclusion:Extended resection led to a greater response in growth factor expression. This finding is important since it shows that growth factor response differs acdording to the extent of resection. We have demonstrated the need to standardize murine hepatic resection models to adequately compare the resulting liver damage.Contexte : Différentes approches ont été proposées pour étudier la régénérescence du foie dans des modèles murins. Nous avons exploré l'effet de divers modèles de résection sur l'atteinte hépatique et les paramètres de la régénérescence chez la souris. Méthodes :Nous avons comparé l'aspect technique des 2 approches les plus couramment utilisées, soit les résections de 50 % et de 70 % du foie. L'atteinte hépatique, déter-minée en fonction de la modification des taux d'alanine aminotransférase et d'aspartate aminotransférase sériques, ainsi que les paramètres de régénérescence VEGF (facteur de croissance endothéliale vasculaire) et FGF-2 (facteur de croissance des fibroblastes) ont été analysés à 6 moments. Un score de vitalité postopératoire a en outre été introduit.

Human Stem Cells Promote Liver Regeneration After Partial Hepatectomy in BALB/C Nude Mice

“…Nevertheless, others have already demonstrated that this mouse line is applicable for studies focusing on liver regeneration after partial hepatectomy. [56][57][58] In accordance with other reports, we were not able to reveal definitive insights into mechanistic interactions and to clarify whether beneficial effects of administered MSC are associated with proposed paracrine effects. Our conclusions are mainly based on correlations.…”

The Influence of Liver Resection on Intrahepatic Tumor Growth

“…It is well known that the amount of hepatic regeneration factors released for liver hypertrophy increases with the extent of liver resection 25,26 . Therefore, a link between liver regeneration factors and tumor cell growth may exist.…”

“…Remove the stay suture and the retractor. 26. Close the fascia with a resorbable 5-0 suture using single button knots.…”

The Role of Adipokines in Surgical Procedures Requiring Both Liver Regeneration and Vascular Occlusion

“…In liver resections with mass deletion of 40–70%, there is a linear relation between the % of tissue removed and the grade of proliferation of hepatocytes. However, resections larger than 70% of liver mass result in an increase in mortality [ 93 ]. Thus, it has been suggested that liver can meet the metabolic demands of the recipients with graft sizes of 40% or greater [ 94 ], and importantly this liver mass is able to tolerate the hyperperfusion occurring after LDLT using SFS grafts [ 95 ].…”