The combined effects of increasing sulfur (S) fertilization rates and drought stress on the yield and compositional parameters of spring wheat on Chernozem soil were studied. In a greenhouse pot experiment, increasing S doses (22.4, 28, 56 kg S/ha) were used with a constant nitrogen (N) dose (112 kg N/ha), resulting in different N:S ratios (1:0.2; 1:0.25; 1:0.5). Water supply treatments included optimal irrigation, maintaining 60% of field capacity, and a water stress treatment where irrigation was withheld until wilting symptoms appeared, followed by irrigation to 40% of field capacity. By measuring the dry biomass production; plant N and S%; and inorganic sulfate-S content, the N/S ratio; harvest index (HI); and organic S, N and S uptake were determined. Our findings indicate that, under water stress, S incorporation into plants is limited, as it tends to remain in an inorganic form. Furthermore, results showed an increase in the N/S ratio under drought conditions, suggesting that drought stress impedes S uptake more significantly than N uptake. In this experiment, fertilization with 112 kg N/ha and 56 kg S/ha (N:S = 1:0.5) proved to be most effective under adequate water supply. In this treatment, grain N and S% were 1.80% and 0.18%, respectively.