Effect of different media and growth hormones on shoot multiplication of in vitro grown Centella asiatica accessions

Abstract

Centella asiatica also known as Gotu Kola is an important medicinal plant which contains several bioactive compounds such as triterpenoid saponins including asiaticoside, madecassoside, centelloside, asiatic acid, etc. In addition, Centella sp. contains other components including flavonoids, phytosterols, tannins, amino acids, sugars, etc. Due to its medicinal importance, this plant is being overexploited and it is essential to conserve this plant. In the present investigation, comparative study of different accessions of Centella asiatica for shoot multiplication in different media and growth hormones was performed. Shoot multiplication and phytocompounds production from different accessions is required to be assessed to choose an accession giving optimum production of phytocompounds. For this, different media containing different combinations of macro and micro nutrients have to be tested that influence the growth of the plant. Assessment of best culture media and concentrations of plant hormone for shoot culture are some of the critical culture conditions to achieve. So there is a need to optimise such conditions that will enhance the growth of the plant. For shoot culture, explant of different accession of Centella asiatica were inoculated in different media i.e. Murashige and Skoog (MS), Gamborg's B5 and Nitsch medium which were supplemented with standard concentrations of plant growth hormones. The cultures were incubated at 25 ± 2°C with photoperiod of 16 hours. After six week of incubation period, highest growth was found in MS media in all accessions. Further MS medium was supplemented with different combinations of growth hormones. After six week of incubation, MS medium supplemented with 1mg/l BAP showed the highest growth of the plant followed by 2mg/l BAP+0.5 NAA.

Estimation of Asiaticoside by Using RP-HPLC and FAME Analysis of Medicinally Important Plant Centella asiatica

“…[5][6][7][8][9][10] which are known to take care of skin problems, to heal wounds, for stimulating the nerves and brain cells, antileprotic, antifilarial, antibacterial, adaptogenic, antifeedant, anti-stress, anti-ulcer, antioxidative stress, anti-radiation properties, anti-heavy metal poisoning, antiviral properties, anticancer etc. [11][12][13][14][15][16][17][18][19][20].…”

Role of medicinal plants against Alzheimer’s disease

“…23 Asiatic acid and asiaticoside showed reduce hydrogen peroxide-induced cell death, decline concentration of free radicals, and β-amyloid cell death inhibition in vitro which suggested possible role in Alzheimer's disease treatment and β-amyloid toxicity prevention. [24][25][26] Extracts of Centella asiatica reversed the β-amyloid pathology in mice brains and modulated oxidative stress response components. 27 It is an important plants for nerve and brain cells and considers being capable of enhancing intellect, memory and longevity.…”

Qualitative and Quantitative Phytochemical Analysis of Centella asiatica

“…In 2006, it was ranked third position in a priority list of most essential Indian medicinal plants based on their pharmaceutical and economic importance and also the demands for its raw material is constantly rising throughout the world [3]. Centella contains several valuable secondary compounds or terpenoids, known as centelloids, which includes asiaticoside, madecassoside, centelloside, centellose, brahminoside, thankunizide, sceffoleoside, brahmoside, and asiatic, centellic, brahmic, and madecassic acids [4][5][6]. The most biologically active compounds include triterpene saponin like asiatic acid, asiaticoside, madecassoside and madecassic acid, which are responsible for a wide range of therapeutic activity [7][8][9].…”