Effect of different methods of isolation of platelets on surface structure and biochemical parameters of their serotonin system

Influence of cell isolation method on the optimization of CD4+ T cell expansion using anti-CD3/CD28 coated beads

“…The activation of cell during the isolation procedure might lead to a reduction of T lymphocyte functions, including proliferation, cytokine production and alteration of the phenotypic characterization of the cells. [15][16][17] In this study, the effect of the cell isolation method on the expansion rate of cells and their phenotypic characterization were determined. The results demonstrate that the CD8 depletion method gave the highest cell recovery compared with CD4 enrichment by negative selection and immunorosette formation.…”

Identification of specific binding sites for nerve growth factor on human blood platelets and membranes from bovine brain

“…Platelets were isolated from the blood by gel filtration [2]. For isolation of the membrane fraction from bovine brain cortex the tissue was homogenized in cooled isolation medium (phosphate buffer saline, PBS, pH 7.4) containing 1 mM PMSF at a 1:10 weight:volume ratio and centrifuged at 750 g for 10 min.…”

Circulating platelet aggregates damage endothelial cells in culture

“…Platelets were isolated either from healthy donors' or sheep jugular vein blood by a modified procedure of Kiktenko et al 15 Briefly, 20 mL of blood was collected into ethylenediaminetetracetic acid tubes and centrifuged at 200g for 25 min to obtain platelet-rich plasma (PRP). PRP was centrifuged at 1500g for 15 min.…”