Effect of different storage conditions on COVID‐19 RT‐PCR results

Güleç, Elif Yılmaz; Cesur, Nevra Pelin; Fazlioğlu, Gonca Yeşilyurt; Kazezoğlu, Cemal

doi:10.1002/jmv.27204

Cited by 30 publications

(25 citation statements)

References 18 publications

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“…In a study by Yilmaz et al. [ 10 ], storage of samples at room temperature or refrigerated conditions did not affect significantly the CT value for the first 3 days, however fourth day onwards in samples stored at room temperature the CT values started to increase. They reported that all positives had increase in their mean CT values at both the temperatures [ 10 ].…”

Section: Discussionmentioning

confidence: 95%

“…In a study by Yilmaz et al. [ 10 ] majority of the samples stored at 4 °C had consistent CT values over 5 days, however CT values increased by 1–4 amplification cycles for most of the samples stored at room temperature over 5 days. In our study though the CT values increased at regular intervals over 5 days there was no significant difference between the increase of the CT values at room temperature or refrigerated conditions.…”

Section: Discussionmentioning

confidence: 98%

“…[ 10 ], storage of samples at room temperature or refrigerated conditions did not affect significantly the CT value for the first 3 days, however fourth day onwards in samples stored at room temperature the CT values started to increase. They reported that all positives had increase in their mean CT values at both the temperatures [ 10 ]. In our study also the mean CT value of all the samples increased at both the temperatures however there was no significant difference in the mean increase of the CT values at the two storage temperatures.…”

Section: Discussionmentioning

confidence: 99%

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Effect of delay in processing and storage temperature on diagnosis of SARS-CoV-2 by RTPCR testing

Anagoni

Mudhigeti

Mohan

et al. 2022

Indian Journal of Medical Microbiology

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of delay in processing and storage temperature on diagnosis of SARS-CoV-2 by RTPCR testing

Anagoni

Mudhigeti

Mohan

et al. 2022

Indian Journal of Medical Microbiology

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“…There nevertheless remains a gap in defining the upper time limit of such storage, particularly with reference to SARS-CoV-2. Thus, there have been several studies evaluating SARS-CoV-2 PCR sample integrity over refrigerator and room temperatures, [5][6][7][8][9] we have not come across any published, publicly available, study or data with which we could perform a direct comparison of the results obtained in this study.…”

Section: Discussionmentioning

confidence: 99%

“…With reference to SARS-CoV-2 detection through PCR in respiratory specimens specifically, there are several studies aimed at evaluating sample integrity following short-term storage at room temperature and at refrigerator temperatures (2-8°C). [5][6][7][8][9] There is, however, a paucity of studies evaluating sample viability upon long-term storage at standard ultra-freezer temperatures (-70 to -80°C). Guidelines for the SARS-CoV-2 PCR kits used in this study also state that respiratory specimen stability at standard refrigerator and freezer temperatures has not been established.…”

Section: Introductionmentioning

confidence: 99%

Duration of respiratory sample stability at -80ºC for SARS-CoV-2 PCR

Aijaz

Naseer²,

Dojki³

et al. 2021

Pak J Med Sci

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Objective: To determine the stability of respiratory samples for SARS-CoV-2 PCR at standard laboratory ultra-freezer temperatures (-80°C). Methods: Five hundred and sixty-five archived, SARS-CoV-2 PCR positive patient specimens received at the Pathology Department of the Indus Hospital & Health Network between January 2021 and June 2021 were retested in June 2021. Samples had been stored at -70°C or below throughout this duration. Sample integrity following storage was assessed as the percentage of samples with reproducible results, and as consistency of cycle threshold (Ct) values between the original testing and the repeat testing. Results: Of the 565 samples evaluated in this study, 86% gave reproducible results upon retesting. However, there was no correlation between the duration of storage and result reproducibility, though the majority (69% for PCR Target-I and 78% for PCR Target-II respectively) of non-reproducible results had Ct values above 30. Similarly, there was a consistent increase of Ct values upon storage at ultra-freezer temperatures, though the effect again was more contingent upon freezing the sample in the ultra-freezer rather than the duration of storage. Conclusion: SARS-CoV-2 positive respiratory specimens for PCR can be stored for up to six months at -70°C or below without loss of sample integrity, though there is some loss of PCR-detected viral targets as evidenced by an immediate increased in the PCR-generated Ct values. In addition, samples with initial Ct values above 30 are more likely to give non-reproducible results. doi: https://doi.org/10.12669/pjms.38.ICON-2022.5777 How to cite this:Aijaz J, Naseer F, Dojki M, Jamal S. Duration of respiratory sample stability at -80ºC for SARS-CoV-2 PCR. Pak J Med Sci. 2022;38(2):393-398. doi: https://doi.org/10.12669/pjms.38.ICON-2022.5777 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Molecular Point‐of‐Care Assay Development: Design and Considerations

Hassman,

Rouchka,

Sunino

et al. 2024

Current Protocols

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Molecular diagnostic point‐of‐care (MDx POC) testing is gaining momentum and is increasingly important for infectious disease detection and monitoring, as well as other diagnostic areas such as oncology. Molecular testing has traditionally required high‐complexity laboratories. Laboratory testing complexity is determined by utilizing the Clinical Laboratory Improvement Amendments of 1988 (CLIA) Categorization Criteria scorecard, utilizing seven criteria that are scored on a scale of one to three. Previously, most commercially available point‐of‐care (POC) tests use other analytes and technologies that were not found to be highly complex by the CLIA scoring system. However, during the COVID‐19 pandemic, MDx POC testing became much more prominent. Utilization during the COVID‐19 pandemic has demonstrated that MDx POC testing applications can have outstanding advantages compared to available non‐molecular POC diagnostic tests. This article introduces MDx POC testing to students, technologists, researchers, and others, providing a general algorithm for MDx POC test development. This algorithm is an introductory, step‐by‐step decision tree for defining a molecular POC diagnostic device meeting the functional requirements for a desired application. The technical considerations driving the decision‐making include nucleic acid selection method (DNA, RNA), extraction methods, sample preparation, number of targets, amplification technology, and detection method. The scope of this article includes neither higher‐order multiplexing, nor quantitative molecular analysis. This article covers key application considerations, such as sensitivity, specificity, turnaround time, and shipping/storage requirements. This article provides an overall understanding of the best resources and practices to use when developing a MDx POC assay that may be a helpful resource for readers without extensive molecular testing experience as well as for those who are already familiar with molecular testing who want to increase MDx availability at the POC. © 2024 Wiley Periodicals LLC.

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Effect of different storage conditions on COVID‐19 RT‐PCR results

Cited by 30 publications

References 18 publications

Effect of delay in processing and storage temperature on diagnosis of SARS-CoV-2 by RTPCR testing

Effect of delay in processing and storage temperature on diagnosis of SARS-CoV-2 by RTPCR testing

Duration of respiratory sample stability at -80ºC for SARS-CoV-2 PCR

Molecular Point‐of‐Care Assay Development: Design and Considerations

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