Effect of different stresses on trehalose levels in <i>Rhizopus oryzae</i>

Uyar, Ebru; Hamamcı, Haluk; Türkel, Sezai

doi:10.1002/jobm.200900339

Cited by 14 publications

(12 citation statements)

References 21 publications

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“…on May 10, 2018 by guest http://aem.asm.org/ stress response (9), and a similar behavior was recently found in Rhizopus oryzae (55). On the other hand, the role of trehalose in protection against cold stress has been extensively studied in plants and the model organisms E. coli and S. cerevisiae (20,30,50).…”

Section: Vol 77 2011 Engineering Trehalose Synthesis In Lactococcussupporting

confidence: 62%

Engineering Trehalose Synthesis in Lactococcus lactis for Improved Stress Tolerance

Carvalho

Cardoso

Böhn

et al. 2011

Appl Environ Microbiol

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Trehalose accumulation is a common cell defense strategy against a variety of stressful conditions. In particular, our team detected high levels of trehalose in Propionibacterium freudenreichii in response to acid stress, a result that led to the idea that endowing Lactococcus lactis with the capacity to synthesize trehalose could improve the acid tolerance of this organism. To this end, we took advantage of the endogenous genes involved in the trehalose catabolic pathway of L. lactis, i.e., trePP and pgmB, encoding trehalose 6-phosphate phosphorylase and ␤-phosphoglucomutase, respectively, which enabled the synthesis of trehalose 6-phosphate. Given that L. lactis lacks trehalose 6-phosphate phosphatase, the respective gene, otsB, from the food-grade organism P. freudenreichii was used to provide the required activity. The trehalose yield was approximately 15% in resting cells and in mid-exponential-phase cells grown without pH control. The intracellular concentration of trehalose reached maximal values of approximately 170 mM, but at least 67% of the trehalose produced was found in the growth medium. The viability of mutant and control strains was examined after exposure to heat, cold or acid shock, and freeze-drying. The trehalose-producing strains showed improved tolerance (5-to 10-fold-higher survivability) to acid (pH 3) and cold shock (4°C); there was also a strong improvement in cell survival in response to heat shock (45°C), and no protection was rendered against dehydration. The insight provided by this work may help the design of food-grade strains optimized for the dairy industry as well as for oral drug delivery.

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Section: Vol 77 2011 Engineering Trehalose Synthesis In Lactococcussupporting

confidence: 62%

Engineering Trehalose Synthesis in Lactococcus lactis for Improved Stress Tolerance

Carvalho

Cardoso

Böhn

et al. 2011

Appl Environ Microbiol

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“…Comparatively, when a preferred carbon source was present, such as glucose, maltose or NAG, Mad2 expression was barely detectable, suggesting that Mad2 is likely under carbon catabolite repression. Trehalose, although a preferred carbohydrate (Rangel et al 2006), is stored in response to nitrogen starvation (Rangel et al 2008;Uyar et al 2010) and thus was not accessible for metabolism until peptone was added to trehalose.…”

Section: Discussionmentioning

confidence: 99%

Differential expression of insect and plant specific adhesin genes, Mad1 and Mad2, in Metarhizium robertsii

Barelli¹,

Padilla-Guerrero²,

Bidochka³

2011

Fungal Biology

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“…Several fungal species have been shown to induce trehalose production as a stress response. Examples include: Saccharomyces cerevisiae [8,9], Zygosaccharomyces bailii [10], A. nidulans [11], A. fumigatus [12], Rhizopus oryzae [13], and Botrytis cinerea [14]. Trehalose is known to protect both proteins and lipid membranes of living cells against stressors such as heat, desiccation and cold.…”

Section: Introductionmentioning

confidence: 99%

Trehalose synthesis in Aspergillus niger: characterization of six homologous genes, all with conserved orthologs in related species

et al. 2014

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BackgroundThe disaccharide trehalose is a major component of fungal spores and is released upon germination. Moreover, the sugar is well known for is protective functions, e.g. against thermal stress and dehydration. The properties and synthesis of trehalose have been well investigated in the bakers’ yeast Saccharomyces cerevisiae. In filamentous fungi, such knowledge is limited, although several gene products have been identified.ResultsUsing Aspergillus niger as a model fungus, the aim of this study was to provide an overview of all genes involved in trehalose synthesis. This fungus has three potential trehalose-6-phosphate synthase encoding genes, tpsA-C, and three putative trehalose phosphate phosphatase encoding genes, tppA-C, of which two have not previously been identified. Expression of all six genes was confirmed using real-time PCR, and conserved orthologs could be identified in related Aspergilli. Using a two-hybrid approach, there is a strong indication that four of the proteins physically interact, as has previously been shown in S. cerevisiae. When creating null mutants of all the six genes, three of them, ΔtpsA, ΔtppA and ΔtppB, had lower internal trehalose contents. The only mutant with a pronounced morphological difference was ΔtppA, in which sporulation was severely reduced with abnormal conidiophores. This was also the only mutant with accumulated levels of trehalose-6-phosphate, indicating that the encoded protein is the main phosphatase under normal conditions. Besides ΔtppA, the most studied deletion mutant in this work was ΔtppB. This gene encodes a protein conserved in filamentous Ascomycota. The ΔtppB mutant displayed a low, but not depleted, internal trehalose content, and conidia were more susceptible to thermal stress.ConclusionA. niger contains at least 6 genes putatively involved in trehalose synthesis. Gene expressions related to germination have been quantified and deletion mutants characterized: Mutants lacking tpsA, tppA or tppB have reduced internal trehalose contents. Furthermore, tppA, under normal conditions, encodes the functional trehalose-6-phosphate-phosphatase.

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Effect of different stresses on trehalose levels in Rhizopus oryzae

Cited by 14 publications

References 21 publications

Engineering Trehalose Synthesis in Lactococcus lactis for Improved Stress Tolerance

Engineering Trehalose Synthesis in Lactococcus lactis for Improved Stress Tolerance

Differential expression of insect and plant specific adhesin genes, Mad1 and Mad2, in Metarhizium robertsii

Trehalose synthesis in Aspergillus niger: characterization of six homologous genes, all with conserved orthologs in related species

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