Effect of difructose anhydride III supplementation on passive immunoglobulin G transfer and serum immunoglobulin G concentration in newborn Holstein calves fed pooled colostrum

Abstract

Difructose anhydride (DFA) III acts on intestinal epithelial tight junctions and promotes calcium absorption through the paracellular pathway in dairy cows. This trial was done to investigate the effects of DFA III supplementation on passive IgG transfer and serum IgG concentration in calves. Twenty-four newborn Holstein male calves were grouped as 12 pairs, with each pair receiving a separate batch of pooled colostrum (PC) from the first 2 milkings. One calf from each pair was allocated to the treatment group (n=12) and received 18g of DFA III in each feeding; the other calf was assigned to the control group (n=12) and did not receive DFA III. All calves were fed 2 L of PC per feeding at 1, 10, and 24 h after birth. Prefeeding blood samples taken at 0 h, 10 h, 24 h, 36 h, 4 d, and 7 d were analyzed for serum IgG concentration. Apparent efficiency of IgG absorption in the treatment group was higher compared to that in the control group. Serum IgG concentration of calves in the treatment group was higher for all sampling time points except for 0 h of age. Mean serum IgG concentrations in the treatment and control groups at 36 h of age were 22.3 and 17.7 g/L, respectively. Serum IgG concentration at 36 h of age was positively related with the IgG concentration in the PC in both groups, and it was higher in the treatment group than in the control group at the same IgG concentration in the PC. These results indicate that absorption of ingested IgG by endocytosis as well as a nonselected concentration gradient process of the paracellular pathway of the intestinal epithelium could be enhanced by DFA III supplementation. Supplementation of DFA III in PC containing various IgG concentrations could improve IgG absorption as well as calf serum IgG concentration.

Effects of difructose anhydride III on serum immunoglobulin G concentration and health status of newborn Holstein calves during the preweaning period

“…at 36 h of age, as reported in previous studies (Weaver et al, 2000;Furman-Fratczak et al, 2011). The peak serum IgG concentration was higher in calves fed colostrum with DFA III than in calves fed colostrum without DFA III, consistent with our previous studies (Sato et al, 2012;Htun et al, 2016). In addition to the peak serum IgG concentration, AEA was higher in the treatment group than in the control group.…”

“…Difructose anhydride (DFA) III is a plant-derived, indigestible oligosaccharide that can promote calcium absorption through paracellular pathways in the intestine of rats (Mineo et al, 2004) and cattle (Teramura et al, 2015). We previously reported that supplementation of DFA III in colostrum fed to newborn calves improved absorption of colostral IgG and increased serum IgG concentrations (Sato et al, 2012;Htun et al, 2016). It has been suggested that the increase of peak serum IgG concentration after colostrum feeding could be due to improved intestinal paracellular pathway via loosening the tight junctions between intestinal epithelial cells by DFA III (Mineo et al, 2004) for greater IgG absorption in newborn calves (Sato et al, 2012;Htun et al, 2016), because DFA III has been shown to improve paracellular absorption in bovine intestine (Teramura et al, 2015).…”

“…We previously reported that supplementation of DFA III in colostrum fed to newborn calves improved absorption of colostral IgG and increased serum IgG concentrations (Sato et al, 2012;Htun et al, 2016). It has been suggested that the increase of peak serum IgG concentration after colostrum feeding could be due to improved intestinal paracellular pathway via loosening the tight junctions between intestinal epithelial cells by DFA III (Mineo et al, 2004) for greater IgG absorption in newborn calves (Sato et al, 2012;Htun et al, 2016), because DFA III has been shown to improve paracellular absorption in bovine intestine (Teramura et al, 2015). If DFA III enhances absorption of colostral IgG through a paracellular pathway, peak serum IgG might increase with DFA III supplementation, regardless of IgG concentration in the colostrum.…”

Difructose Anhydride and Passive Immunity Effects on Passive Immune Transfer and Performance of Feeding Difructose Anhydride to Neonatal Calves

“…The mechanisms behind the improvement in EAE are unknown. Several authors have speculated that the increased absorption of IgG in calves supplemented with DFAIII could be due to an improvement in intestinal absorption through the paracellular pathway by increasing the opening of the epithelial tight junctions [ 22 , 32 , 33 ]. However, Jochims et al [ 19 ] clearly illustrated that intestinal absorption of IgG in calves occurred through micropinocytic transfer.…”