2005
DOI: 10.1111/j.1751-1097.2005.tb01455.x
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Effect of Digitonin on the Rhodopsin Meta I‐Meta II Equilibrium

Abstract: Absorbance difference spectra were recorded from 10 μs to 540 ms after photoexcitation of sonicated suspensions of hypotonically washed bovine rod outer segments with varying amounts of the detergent digitonin added (0 to 2%) at 20°C. Metarhodopsin I480 and metarhodopsin II displayed the expected anomalous pH dependence at pH 6 and 8 (i.e. opposite to that expected from direct protonation of the chromophore Schiff base). However, increasing levels of digitonin eliminated the pH dependence of the equilibrium, a… Show more

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