Effect of dimethylaminohexadecyl methacrylate mass fraction on fracture toughness and antibacterial properties of CaP nanocomposite

Abstract

Objectives Biofilm acids contribute to secondary caries which is a reason for restoration failure. Previous studies synthesized nanoparticles of amorphous calcium phosphate (NACP) and dimethylaminohexadecyl methacrylate (DMAHDM). The objectives of this study were to develop DMAHMD-NACP nanocomposite for double benefits of antibacterial and remineralization capabilities, and investigate the DMAHMD mass fraction effects on fracture toughness and biofilm response of NACP nanocomposite for the first time. Methods DMAHDM was incorporated into NACP nanocomposite at mass fractions of 0% (control), 0.75%, 1.5%, 2.25% and 3%. A single edge V-notched beam method was used to measure fracture toughness Kic. A dental plaque microcosm biofilm model using human saliva as inoculum was used to measure the antibacterial properties of composites. Results Kic was about 1 MPa·m1/2 for all composite (mean ± sd; n = 6). Adding DMAHDM from 0% to 3% did not affect Kic (p > 0.1). Lactic acid production by biofilms on composite containing 3% DMAHDM was reduced to less than 1% of that on composite control. Metabolic activity of adherent biofilms on composite containing 3% DMAHDM was reduced to 4% of that on composite control. Biofilm colony-forming unit (CFU) counts were reduced by three orders of magnitude on NACP nanocomposite containing 3% DMAHDM. Conclusions DMAHDM-NACP nanocomposite had good fracture resistance, strong antibacterial potency, and NACP for remineralization (shown in previous studies). The DMAHDM-NACP nanocomposite may be promising for caries-inhibiting dental restorations, and the method of using double agents (DMAHDM and NACP) may have a wide applicability to other dental materials including bonding agents and cements.

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

“…remineralization or protein-repellent properties). Some research groups have incorporated additional components such as AgNPs [268][269][270], NOreleasing agents [173,174], fluoride [414], NACPs [232][233][234][235][236][237]335] and 2-methacryloyloxyethyl phosphorylcholine [415][416][417] into QA-based biomedical materials to achieve augmented antimicrobial or multifunctional applications.…”

Antibacterial Properties of Nanoparticles in Dental Restorative Materials. A Systematic Review and Meta-Analysis

“…Again, these studies were published by interrelated teams of authors. The second was made up of studies that integrated nanoparticles into resin-based materials [45][46][47][48][49][50]; all of them stressed that the nanoparticles were incorporated into a resin already containing amorphous calcium phosphate nanoparticles (NaCP nanoparticles). As in the previous subgroup, the teams of authors were interrelated, the only difference between the works being the type of monomer (dimethylaminohexane methacrylate (DMAHDM) or dimethylaminododecyl methacrylate (DMADDM)) incorporated into the resin.…”

A review of bioceramics-based dental restorative materials

“…The search showed that a total of 49 studies 8,10,12,[16][17][18][19] had been conducted related to ACP-based resin restorative materials. Among these nano-amorphous calcium phosphate (NACP) had been used in 15 studies 19,[21][22][23][24][25][26][27][28][29][30][31]57,58,62) , and the rest were based on micro-ACP. Moreover, zirconia 8,[32][33][34][35][36][37][38][39][40] and silica 8,39,41) had been hybridized with ACP to improve the mechanical and biological properties Nano-silver particles 27,57) and HA powder 42) were also mixed with ACP to analyze the antibacterial and mechanical properties, respectively.…”