Effect of dimethylsulfoxide on the functions of mesenchymal and hemopoietic precursors

Abstract

The effects of dimethylsulfoxide on the state of mesenchymal precursors in vivo were demonstrated. Treatment with dimethylsulfoxide reduced the content of stromal clonogenic elements in the bone marrow and inhibited mobilization of mesenchymal precursors induced by granulocyte colony-stimulating factor. In in vitro system, dimethylsulfoxide inhibited proliferation of fibroblast, erythroid, and granulomonocytic colony-forming units and stimulates maturation of hemopoietic precursors.

Involvement of cAMP- and IKK-2-Dependent Signal Pathways in the Growth Capacity of Mesenchymal Progenitor Cells under the Influence of Basic Fibroblast Growth Factor

“…However, a specifi c inhibitor of this signal system had no effect on functional activity of CFU-F (Fig. 1, a) that were presented by early (e.g., multipotent) progenitor cells [5,10,11]. At the same time, 2',5'-dideoxyadenosine signifi cantly increased the yield of stromal-determined progenitor cells.…”

“…Culture methods were used to study the in vitro direct effect of an adenylate cyclase inhibitor 2',5'-dideoxyadenosine (Calbiochem) and IKK-2 blocker (one of two extremely homologous kinase subunits, IKK) inhibitor IV (Calbiochem) on the growth of fi broblast CFU (CFU-F) and cluster-forming units (ClFU-F) [11] in the culture of bone marrow cells after addition of 20 ng/ml bFGF (Sigma). The in vitro working concentrations of inhibitors were estimated to be most effective (30 and 0.02 μM, respectively).…”

“…The intensity of CFU-F differentiation was evaluated from the index of maturation. This index was calculated as the ratio of ClFU-F (focus of mesenchymopoiesis, 5-50 cells) to CFU-F (more than 50 cells; index of differentiation) in the tissue culture [2,10,11].…”

“…The Pharmacology Strategy of Regenerative Medicine was developed. This strategy is based on the principle of functional imitation of natural regulatory systems [6,7,10,11,15]. Key stages of the signal cascade, which is involved in functional stimulation of progenitor cells by endogenous regulators of stem cell function, hold much promise as a target for treatment [2,10].…”

Myocyte Enhancer Factor 2C, an Osteoblast Transcription Factor Identified by Dimethyl Sulfoxide (DMSO)-enhanced Mineralization

“…DMSO has other effects in bone biology. Zyuz'kov et al (31) show that DMSO treatment (for 5 days at 2 g/kg) via a gastric tube in mice results in a decrease of colony-forming units-fibroblast (CFU-F) derived from the stromal fraction in bone marrow cultures at 8 days. We also tested DMSO on osteoclast differentiation and found profound stimulation, particularly in the size of osteoclasts.…”