Effect of Diosmin on Selected Parameters of Oxygen Homeostasis

Feldo, Marcin; Wójciak, Magdalena; Dresler, Sławomir; Sowa, Paweł; Płachno, Bartosz J.; Samborski, Dariusz; Sowa, Ireneusz

doi:10.3390/ijms241612917

Cited by 1 publication

(2 citation statements)

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“…Diosmin is a glycosylated polyphenolic flavonoid found in Citrus aurantium (Citrus sinensis) and olive leaves (Olea europaea) [ 16 – 18 ]. Several clinical studies have demonstrated the anti-inflammatory potential of Diosmin [ 19 , 20 ]. Diosmin can inhibite levels of TNF-α, IL-1β, and IL-6 while promoting IL-12 production in patients with chronic venous disease [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Several clinical studies have demonstrated the anti-inflammatory potential of Diosmin [ 19 , 20 ]. Diosmin can inhibite levels of TNF-α, IL-1β, and IL-6 while promoting IL-12 production in patients with chronic venous disease [ 19 ]. In preclinical studies, Diosmin has also shown a range of pharmacological activities including antioxidant, anti-inflammatory and anti-apoptotic [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Diosmin ameliorates renal fibrosis through inhibition of inflammation by regulating SIRT3-mediated NF-κB p65 nuclear translocation

Zhao,

Li,

Zhu

et al. 2024

BMC Complement Med Ther

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Background Renal fibrosis is considered an irreversible pathological process and the ultimate common pathway for the development of all types of chronic kidney diseases and renal failure. Diosmin is a natural flavonoid glycoside that has antioxidant, anti-inflammatory, and antifibrotic activities. However, whether Diosmin protects kidneys by inhibiting renal fibrosis is unknown. We aimed to investigate the role of Diosmin in renal interstitial fibrosis and to explore the underlying mechanisms. Methods The UUO mouse model was established and gavaged with Diosmin (50 mg/kg·d and 100 mg/kg·d) for 14 days. HE staining, Masson staining, immunohistochemistry, western blotting and PCR were used to assess renal tissue injury and fibrosis. Elisa kits were used to detect the expression levels of IL-1β, IL-6, and TNF-α and the activity of SIRT3 in renal tissues. In addition, enrichment maps of RNA sequencing analyzed changes in signaling pathways. In vitro, human renal tubular epithelial cells (HK-2) were stimulated with TGF-β1 and then treated with diosmin (75 μM). The protein and mRNA expression levels of SIRT3 were detected in the cells. In addition, 3-TYP (selective inhibitor of SIRT3) and SIRT3 small interfering RNA (siRNA) were used to reduce SIRT3 levels in HK-2. Results Diosmin attenuated UUO-induced renal fibrosis and TGF-β1-induced HK-2 fibrosis. In addition, Diosmin reduced IL-1β, IL-6, and TNF-α levels in kidney tissues and supernatants of HK-2 medium. Interestingly, Diosmin administration increased the enzymatic activity of SIRT3 in UUO kidneys. In addition, Diosmin significantly increased mRNA and protein expression of SIRT3 in vitro and in vivo. Inhibition of SIRT3 expression using 3-TYP or SIRT3 siRNA abolished the anti-inflammatory effects of diosmin in HK-2 cells. Enrichment map analysis by RNA sequencing indicates that the nuclear factor-kappa B (NF-κB) signaling pathway was inhibited in the Diosmin intervention group. Furthermore, we found that TGF-β1 increased the nuclear expression of nuclear NF-κB p65 but had little significant effect on the total intracellular expression of NF-κB p65. Additionally, Diosmin reduced TGF-β1-caused NF-κB p65 nuclear translocation. Knockdown of SIRT3 expression by SIRT3 siRNA increased the nuclear expression of NF-κB p65 and abolished the inhibition effect of Diosmin in NF-κB p65 expression. Conclusions Diosmin reduces renal inflammation and fibrosis, which is contributed by inhibiting nuclear translocation of NF-κB P65 through activating SIRT3.

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