Effect of direct plasma treatment and plasma‐treated liquid on cutaneous squamous cell carcinoma: A proof‐of‐concept study

Özdemir, Gazi; Ercan, Utku Kürşat; Karaman, Ozan; Oltulu, Fatih

doi:10.1002/ppap.202200246

Cited by 8 publications

(4 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The human keratinocyte (HS2) cell line and squamous cell carcinoma (SCC) cell lines were treated with CAP operated at peak-to-peak voltage and 1 kHz frequency at different treatment times. In vitro wound healing images were obtained at 24-hour intervals at 40x and 100x magnifications using the same inverted microscope (Olympus CKX41) [ 32 ]. Among the 167 CAP-treated images, 133 were of the HS2 cell line, of which 67 were captured using a 40x magnification and 66 using a 100x magnification setting.…”

Section: Methodsmentioning

confidence: 99%

An automated in vitro wound healing microscopy image analysis approach utilizing U-net-based deep learning methodology

Doğru,

Özdemir,

Özdemir

et al. 2024

BMC Med Imaging

Self Cite

View full text Add to dashboard Cite

Background The assessment of in vitro wound healing images is critical for determining the efficacy of the therapy-of-interest that may influence the wound healing process. Existing methods suffer significant limitations, such as user dependency, time-consuming nature, and lack of sensitivity, thus paving the way for automated analysis approaches. Methods Hereby, three structurally different variations of U-net architectures based on convolutional neural networks (CNN) were implemented for the segmentation of in vitro wound healing microscopy images. The developed models were fed using two independent datasets after applying a novel augmentation method aimed at the more sensitive analysis of edges after the preprocessing. Then, predicted masks were utilized for the accurate calculation of wound areas. Eventually, the therapy efficacy-indicator wound areas were thoroughly compared with current well-known tools such as ImageJ and TScratch. Results The average dice similarity coefficient (DSC) scores were obtained as 0.958$$\sim$$ ∼ 0.968 for U-net-based deep learning models. The averaged absolute percentage errors (PE) of predicted wound areas to ground truth were 6.41%, 3.70%, and 3.73%, respectively for U-net, U-net++, and Attention U-net, while ImageJ and TScratch had considerable averaged error rates of 22.59% and 33.88%, respectively. Conclusions Comparative analyses revealed that the developed models outperformed the conventional approaches in terms of analysis time and segmentation sensitivity. The developed models also hold great promise for the prediction of the in vitro wound area, regardless of the therapy-of-interest, cell line, magnification of the microscope, or other application-dependent parameters.

show abstract

Section: Methodsmentioning

confidence: 99%

An automated in vitro wound healing microscopy image analysis approach utilizing U-net-based deep learning methodology

Doğru,

Özdemir,

Özdemir

et al. 2024

BMC Med Imaging

Self Cite

View full text Add to dashboard Cite

show abstract

“…High basal levels of ROS in cancer cells have been suggested as a possible cause of CAP inducing selective apoptosis in cancer cells [52]. ROS are aerobic metabolites produced by a variety of intracellular and extracellular factors.…”

Section: Cap In Cancer Therapymentioning

confidence: 99%

“…Additionally, aquaporins (AQP) in cell membranes appear to be involved in CAP-induced selective apoptosis of cancer cells [52]. AQP is a water transport channel protein found in cell membranes, and 13 types (AQP 0-12) are known in mammals [66,67].…”

Section: Cap In Cancer Therapymentioning

confidence: 99%

Clinical application of cold atmospheric-pressure plasma: mechanisms and irradiation conditions

Jeong,

Park,

Lee

et al. 2024

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

Cold atmospheric plasma (CAP) has rapidly advanced as a pivotal area in medical research, notably in wound healing and cancer therapy. This review presents an overview of the mechanisms underlying the action of CAP on wound healing and cancer treatment. CAP plays opposing roles in wound healing and cancer treatment. In wound healing, CAP promotes cell migration and proliferation and eradicates pathogens near the wound site. In cancer therapy, CAP has been recognized for its ability to induce cell death through multiple mechanisms. These include activating the mitochondrial pathway, provoking endoplasmic reticulum stress, generating reactive oxygen species (ROS) and reactive nitrogen species (RNS), causing DNA damage, arresting the cell cycle, and modulating signaling pathways. Additionally, CAP’s oxidative stress can lead to significant changes in DNA and RNA within the nucleus, further contributing to its anti-cancer properties. These diverse effects underscore CAP’s therapeutic potential, though outcomes may differ based on the type of cancer and experimental settings. Furthermore, we compared the CAP application conditions for wound healing and cancer cell treatment. The type and concentration of ROS and RNS depend on plasma generation and treatment conditions. Thus, we aimed to identify the correlations between plasma properties and mechanisms of action by comparing CAP generation and treatment conditions in wound healing and cancer cell treatment.

show abstract

“…The discovery of anticancer properties of CAP has led investigations into its effects on various types of tumors and their underlying mechanisms. It has been observed to effectively induce cancer cell death in numerous human cancer cell lines, including those from the brain [10], hepatoma [11], breast [12], pancreatic [13], colorectal [14], lung cancer [15], leukemia [16], and squamous cell carcinoma (SCC) [17]. The specificity of CAP towards cancer cells makes it a promising alternative to conventional treatments.…”

Section: Introductionmentioning

confidence: 99%

Cold atmospheric plasma (CAP) treatment increased reactive oxygen and nitrogen species (RONS) levels in tumor samples obtained from patients with low-grade glioma

Aggarwal,

Vaid,

Visani

et al. 2024

Biomed. Phys. Eng. Express

View full text Add to dashboard Cite

Low-grade gliomas (LGGs) are a heterogeneous group of tumors with an average 10-year survival rate of 40-55%. Current treatment options include chemotherapy, radiotherapy, and gross total resection (GTR) of the tumor. The extent of resection (EOR) plays an important role in improving surgical outcomes. However, the major obstacle in treating low-grade gliomas is their diffused nature and the presence of residual cancer cells at the tumor margins post resection. Cold Atmospheric Plasma (CAP) has shown to be effective in targeted killing of tumor cells in various glioma cell lines without affecting non-tumor cells through Reactive Oxygen and Nitrogen Species (RONS). However, no study on the effectiveness of CAP has been carried out in LGG tissues till date. In this study, we applied helium-based CAP on tumor tissues resected from LGG patients. Our results show that CAP is effective in promoting RONS accumulation in LGG tissues when CAP jet parameters are set at 4kV voltage, 5min treatment time and 3lpm gas flow rate. We also observed that CAP jet is more effective in thinner slice preparations of tumor as compared to thick tumor samples. Our results indicate that CAP could prove to be an effective adjunct therapy in glioma surgery to target residual cancer cells to improve surgical outcome of patients with low-grade glioma.

show abstract

Effect of direct plasma treatment and plasma‐treated liquid on cutaneous squamous cell carcinoma: A proof‐of‐concept study

Cited by 8 publications

References 64 publications

An automated in vitro wound healing microscopy image analysis approach utilizing U-net-based deep learning methodology

An automated in vitro wound healing microscopy image analysis approach utilizing U-net-based deep learning methodology

Clinical application of cold atmospheric-pressure plasma: mechanisms and irradiation conditions

Cold atmospheric plasma (CAP) treatment increased reactive oxygen and nitrogen species (RONS) levels in tumor samples obtained from patients with low-grade glioma

Contact Info

Product

Resources

About