Effect of disinfectants and manual wiping for processing the cell product changeover in a biosafety cabinet

Mizuno, Mitsuru; Matsuda, Junji; Watanabe, Ken; Shimizu, Norio; Sekiya, Ichiro

doi:10.1016/j.reth.2023.01.009

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…Therefore, it is very important to check and inform CPOs in advance whether they have any respiratory conditions or are allergic to alcohol or other disinfectants. As alcohols or other disinfectants such as ethanol are not always the best disinfectants for cell product manufacturing sites that use high-protein serum or human tissue [ 35 ], it may be necessary to consider alternative methods as well. A survey estimated that 50% of CPOs working in the country had two to three years of work experience, suggesting a high attrition rate [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Volatile organic compounds and ionic substances contamination in cell processing facilities during rest period; a preliminary assessment of exposure to cell processing operators

Mizuno

Abe

Kakimoto

et al. 2023

Regenerative Therapy

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Section: Discussionmentioning

confidence: 99%

Volatile organic compounds and ionic substances contamination in cell processing facilities during rest period; a preliminary assessment of exposure to cell processing operators

Mizuno

Abe

Kakimoto

et al. 2023

Regenerative Therapy

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“…Sterilization of the area within the Biosafety Cabinet (BSC) and gloves when handling breast cancer cells with 70% ethanol is essential to keep contamination at a minimum. Studies have shown that benzalkonium chloride with corrosive inhibition and distilled water in wet conditions would be the optimal combination for the sterilization of the BSC area with respect to a specific bacterium [122]. Considering that benzalkonium chloride with corrosive inhibition may not be the optimal option for most laboratories, 70% ethanol is a common choice for use when sterilizing surfaces.…”

Section: Cross-contaminationmentioning

confidence: 99%

Molecular, Cellular, and Technical Aspects of Breast Cancer Cell Lines as a Foundational Tool in Cancer Research

Witt,

Tollefsbol

2023

Life

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Breast cancer comprises about 30% of all new female cancers each year and is the most common malignant cancer in women in the United States. Breast cancer cell lines have been harnessed for many years as a foundation for in vitro analytic studies to understand the use of cancer prevention and therapy. There has yet to be a compilation of works to analyze the pitfalls, novel discoveries, and essential techniques for breast cancer cell line studies in a scientific context. In this article, we review the history of breast cancer cell lines and their origins, as well as analyze the molecular pathways that pharmaceutical drugs apply to breast cancer cell lines in vitro and in vivo. Controversies regarding the origins of certain breast cancer cell lines, the benefits of utilizing Patient-Derived Xenograft (PDX) versus Cell-Derived Xenograft (CDX), and 2D versus 3D cell culturing techniques will be analyzed. Novel outcomes from epigenetic discovery with dietary compound usage are also discussed. This review is intended to create a foundational tool that will aid investigators when choosing a breast cancer cell line to use in multiple expanding areas such as epigenetic discovery, xenograft experimentation, and cancer prevention, among other areas.

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“…Class I BSCs are essential to ensure personnel safety and prevent the spread of contaminants. Distilled water, benzalkonium chloride (BKC) with corrosion inhibitor, ethanol, peracetic acid, hydrogen peroxide, and ultraviolet (UV) radiation are commonly used to maintain the sterility of class l BSCs before and after sample processing [ 24 ].…”

Section: Reviewmentioning

confidence: 99%

The Role of the Biosafety Cabinet in Preventing Infection in the Clinical Laboratory

Jagtap,

Badge,

Kohale

et al. 2023

Cureus

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Clinical laboratories are essential in healthcare to better diagnose, treat, and track medical diseases. However, handling infectious organisms and possibly infectious materials in these laboratories puts the safety of laboratory workers and the general public at risk. By controlling the distribution of infectious substances and stopping the spread of diseases, biosafety cabinets (BSCs) have become crucial tools in guaranteeing laboratory safety. The prevention of infections is most important in medical and laboratory settings. In clinical laboratories, biological and infectious agents are handled, posing threats to healthcare workers and the general public. To avoid infections, proper training of the BSC is essential. Laboratory employees are instructed in aseptic procedures, proper hand posture, and efficient personal protection when working in the cabinet. These instructions decrease the chance of contaminating the surrounding area. Additionally, user ergonomics are taken into account while designing BSC, reducing operator fatigue, and guaranteeing that staff can execute tasks precisely for extended periods. This review highlights the importance of biosafety cabinets in maintaining a secure laboratory environment and explains their crucial function in infection control.

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Effect of disinfectants and manual wiping for processing the cell product changeover in a biosafety cabinet

Cited by 7 publications

References 29 publications

Volatile organic compounds and ionic substances contamination in cell processing facilities during rest period; a preliminary assessment of exposure to cell processing operators

Volatile organic compounds and ionic substances contamination in cell processing facilities during rest period; a preliminary assessment of exposure to cell processing operators

Molecular, Cellular, and Technical Aspects of Breast Cancer Cell Lines as a Foundational Tool in Cancer Research

The Role of the Biosafety Cabinet in Preventing Infection in the Clinical Laboratory

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