Effect of disinfectants on survival of cryptosporidium oocysts

Campbell, Iain; Tzipori, AS; Hutchison, G.; Angus, KW

doi:10.1136/vr.111.18.414

Cited by 183 publications

(85 citation statements)

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“…In this study many oocysts were trapped in intestinal mucus, and the mucolytic agent Sputolysin was used to free them. PBS was used as an alternative to 10% formol saline in the initial concentration procedure as formalin is one of the few disinfectants capable of inactivating Cryptosporidium oocysts (12). Hypochlorite solutions were not used in the purification procedure because hypochlorite has been shown to damage the integrity of the oocyst wall (13).…”

Section: Discussionmentioning

confidence: 99%

Electrophoretic and immunoblot analysis of Cryptosporidium oocysts

1988

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Summary Cryptosporidium oocysts were recovered by density gradient centrifugation from diarrhoeal faeces of four human patients and one goat kid. Goat-derived oocysts were further treated with excystation medium and the excysted oocyst walls purified by isopycnic ultracentrifugation. Soluble extracts from intact oocysts and the oocyst wall preparation were analysed by SDS-PAGE. Fifty-one polypeptide bands were detected in intact oocyst preparations: 48 were in the range 14 000-200 000 molecular weight (MW), two bands were less than 14 000 MW and one band was above 200 000 MW. Twenty-one bands were detected in the oocyst wall preparation, all within the range 14 000-200 000 MW. Immunoblot analysis oi Cryptosporidium polypeptides using acute or convalescent human and goat sera revealed a large number of reactive bands. Varying degrees of heterogeneity were observed within and between the two semm groups. Nine ofthe 10 human sera and all ofthe goat kid sera reacted with a 23 000 MW and 32 000 MW antigen. A 15 500 MW antigen was also detected by all the goat and four ofthe 10 human sera. Both serum groups reacted with various antigens above 40 000 MW. Surface labelling of three human isolatesofC/^p/o^^^onW/um oocysts with '25i was performed using the Boltonand Hunter reagent. The solubilized preparations were separated by SDS-PAGE on 12% and 18% slab gels and autoradiographed. Common bands were seen at 15 500, 32 000. 47 500, 79 000 and 96 000 MW. Some variation in the molecular weight of polypeptides labelled with ^'^H was observed among the three isolates. These studies indicate that the 32 000 MW antigen appears to be a suitable candidate for the preparation of an antibody probe because it is common to many isolates and is located on the exterior of the oocyst wall.

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Section: Discussionmentioning

confidence: 99%

Electrophoretic and immunoblot analysis of Cryptosporidium oocysts

1988

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“…Rigorous sanitary procedures and disinfection of all objects and surfaces with hypochloric acid was effective in preventing continued Cryptosporidium-infection and disease in quails (Hoerr et al, 1986). Formol saline (10%) and ammonia (5%) have also been recommended for disinfection of premises contaminated with Cryptosporidium oocysts (Campbell et al, 1982;Sundermann et al, 1987). A vaccine against Cryptsoporidium is not available.…”

Section: Ma Goodwinmentioning

confidence: 99%

Cryptosporidiosis in birds ‐ A review

Goodwin¹

1989

Avian Pathology

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“…Contamination of water supplies, directly or by such practices, occurs and oocysts are known to be relatively resistant to chlorine (Campbell et al 1982).…”

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confidence: 99%