Effect of dispersants on bacterial colonization of oil droplets: A microfluidic approach

Hickl, Vincent; Juárez, Gabriel

doi:10.1016/j.marpolbul.2022.113645

Cited by 11 publications

(10 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, fundamentally, the emergent phenomena observed in our experiments are driven by by the division of densely packed, rod-shaped cells at a deformable interface. In our previous work, the doubling time of dilute cells on a flat oil-water interface was measured to have a mean of 1.8 ± 0.1 h [49]. The slower doubling times observed here suggest that cells at the interface divide more slowly when they are densely packed (such as during tube formation) than when they are dilute.…”

Section: A Tubulation and Bio-aggregate Expansionsupporting

confidence: 42%

“…A custom microfluidic chamber was constructed to visualize the time evolution of oil droplets colonized by bacteria [49]. To create droplets on a glass microscope slide, a thin film of 10 µL of either unweathered MC252 crude oil or mineral oil was formed.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…The self-organization and collective behavior of growing bacteria at liquid interfaces has recently been shown to lead to the colonization and buckling of oil droplets [48,49]. Here, we describe the extreme deformations of the oil-water interface and dispersion of oil caused by the growth of bacteria on spherical droplets.…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Tubulation and dispersion of oil by bacterial growth on droplets

Hickl,

Juarez

2022

Preprint

Self Cite

View full text Add to dashboard Cite

Bacteria on surfaces exhibit collective behaviors, such as active turbulence and active stresses, which result from their motility, growth, and interactions with their local surroundings. However, interfacial deformations on soft surfaces and liquid interfaces caused by active growth, particularly over long time scales, are not well understood. Here, we describe experimental observations on the emergence of tubular structures arising from the growth of rod-shaped bacteria at the interface of oil droplets in water. Using microfluidics and timelapse microscopy, the dimensions and extension rates of individual tubular structures as well as bulk bio-aggregate formation are quantified for hundreds of droplets over 72 hours. Tubular structures are comparable in length to the initial droplet radius and are composed of an outer shell of bacteria that stabilize an inner filament of oil.The oil filament breaks up into smaller microdroplets dispersed within the bacterial shell. This work provides insight into active stresses at deformable interfaces and improves our understanding of microbial oil biodegradation and its potential influence on the transport of droplets in the ocean water column.

show abstract

Section: A Tubulation and Bio-aggregate Expansionsupporting

confidence: 42%

Section: Experimental Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Tubulation and dispersion of oil by bacterial growth on droplets

Hickl,

Juarez

2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The droplet size range was 1 mm o R o 200 mm. 50 The caps contained 93 AE 3% of the volume of a sphere with the same diameter, on average. Here, the radius of a droplet refers to the radius of curvature of the spherical cap, measured from the widest cross-section (i.e., the droplet midplane) in microscopy images of each droplet.…”

Section: Experimental Methodsmentioning

confidence: 99%

“…The self-organization and collective behavior of growing bacteria at liquid interfaces has recently been shown to lead to the colonization and buckling of oil droplets. 49,50 Here, we describe the extreme deformations of the oil-water interface and dispersion of oil caused by the growth of bacteria on spherical droplets. Using microfluidics and timelapse microscopy, hundreds of oil droplets of sizes 5 mm r R r 100 mm are observed simultaneously and continuously over 72 hours.…”

Section: Introductionmentioning

confidence: 99%