Effect of dispersants on the biodegradation of South Louisiana crude oil at 5 and 25 °C

Zhuang, Mobing; Abulikemu, Gulizhaer; Campo, Pablo; Platten, William E.; Suidan, Makram T.; Venosa, Albert D.; Conmy, Robyn N.

doi:10.1016/j.chemosphere.2015.08.040

Cited by 16 publications

(8 citation statements)

References 18 publications

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“…Microcosm setup. Two different enrichment cultures were generated from samples collected near the site of the Deepwater Horizon oil spill (40). One culture was derived from a surface sample and the other was derived from a deep-water sample.…”

Section: Methodsmentioning

confidence: 99%

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Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms

Techtmann

Zhuang

Campo

et al. 2017

Appl Environ Microbiol

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To better understand the impacts of Corexit 9500 on the structure and activity levels of hydrocarbon-degrading microbial communities, we analyzed next-generation 16S rRNA gene sequencing libraries of hydrocarbon enrichments grown at 5 and 25°C using both DNA and RNA extracts as the sequencing templates. Oil biodegradation patterns in both 5 and 25°C enrichments were consistent with those reported in the literature (i.e., aliphatics were degraded faster than aromatics). Slight increases in biodegradation were observed in the presence of Corexit at both temperatures. Differences in community structure were observed between treatment conditions in the DNA-based libraries. The 25°C consortia were dominated by ,, ,, and species, while the 5°C consortia were dominated by several species of the genera, , and Most of these genera have been linked to hydrocarbon degradation and have been observed after oil spills. and, known aromatic degraders, were also found in these enrichments. The addition of Corexit did not have an effect on the active bacterial community structure of the 5°C consortia, while at 25°C, a decrease in the relative abundance of was observed. At 25°C,, , and were present at higher relative abundances in the RNA than DNA libraries, suggesting that they were active in degradation. Similarly, was greatly stimulated by the addition of oil at 5°C. While dispersants such as Corexit 9500 can be used to treat oil spills, there is still debate on the effectiveness on enhancing oil biodegradation and its potential toxic effect on oil-degrading microbial communities. The results of this study provide some insights on the microbial dynamics of hydrocarbon-degrading bacterial populations in the presence of Corexit 9500. Operational taxonomic unit (OTU) analyses indicated that several OTUs were inhibited by the addition of Corexit. Conversely, a number of OTUs were stimulated by the addition of the dispersant, many of which were identified as known hydrocarbon-degrading bacteria. The results highlight the value of using RNA-based methods to further understand the impact of dispersant on the overall activity of different hydrocarbon-degrading bacterial groups.

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Section: Methodsmentioning

confidence: 99%

“…Experiments were conducted in triplicates per treatment with a total of 100 ml of medium in 250-ml flasks. The contents of flasks incubated for 0, 2,4,8,12,16,24,32,40, and 48 days were used to measure hydrocarbon residual concentrations and to conduct molecular analyses. Similar measurements were conducted for the cryo enrichment at 56 days.…”

Section: Methodsmentioning

confidence: 99%

Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms

Techtmann

Zhuang

Campo

et al. 2017

Appl Environ Microbiol

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“…Other publications have highlighted UV and bacterial activity as likely instigators of DOSS degradation in aqueous solution (Campo et al ; Batchu et al ; Zhuang et al ). Photostimulated DOSS hydrolysis degradation reactions were previously reported as having 14‐ and 6.5‐d half‐lives under constant exposure to UV light of 350 nm and simulated sunlight (300–800 nm), respectively (Batchu et al ).…”

Section: Resultsmentioning

confidence: 99%

Acute toxicity of Corexit EC9500A and assessment of dioctyl sulfosuccinate as an indicator for monitoring four oil dispersants applied to diluted bitumen

MacInnis

Brunswick

Park

et al. 2018

Enviro Toxic and Chemistry

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The present study investigated oil dispersant toxicity to fish species typical of the cooler regions of Canada, together with less well-documented issues pertaining to oil dispersant monitoring. The oil dispersant toxicity of Corexit EC9500A was assessed for the freshwater fish species rainbow trout and the seawater species coho, chinook, and chum, with a final median lethal concentration (LC50) acute lethality range between 35.3 and 59.8 mg/L. The LC50 range was calculated using confirmed 0-h dispersant concentrations that were justified by fish mortality within the first 24 h of exposure and by variability of the dispersant indicator dioctyl sulfosuccinate (DOSS) used to monitor concentrations at later time points. To investigate DOSS as an oil dispersant indicator in the environment, microcosm systems were prepared containing Corexit EC9500A, Finasol OSR52, Slickgone NS, and Slickgone EW dispersants together with diluted bitumen. The DOSS indicator recovery was found to be stable for up to 13 d at 5 °C, 8 d at 10 °C, but significantly less than 8 d at ≥15 °C. After 3 d at temperatures ≥15 °C, the DOSS indicator recovery became less accurate and was dependent on multiple environmental factors including temperature, microbial activity, and aeration, with potential for loss of solvents and stabilizers. A final assessment determined DOSS to be a discrepant indicator for long-term monitoring of oil dispersant in seawater. Environ Toxicol Chem 2018;37:1309-1319. © 2018 SETAC.

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“…Dispersants are commonly employed in emergency response to marine oil spills to promote the dissolution of oil into water and reduce the transport of hydrocarbons to shoreline ecosystems . Sufficient data are available with respect to the potential effects of dispersants on oil biodegradation …”

Section: Introductionmentioning

confidence: 99%

“…15 Sufficient data are available with respect to the potential effects of dispersants on oil biodegradation. [16][17][18][19][20][21] Compound-specific stable isotope analysis (CSIA) is one of the most promising tools for monitoring and quantifying the organic contaminants of biodegradation. It can be used to fingerprint and evaluate the sources of contaminants, provide a footprint for the scope of degradation, indicate the underlying degradation mechanisms, and identify degradation pathways.…”

Section: Introductionmentioning

confidence: 99%

Microbial degradation of four dispersed crude oils by Rhodococcus sp. evaluated using carbon stable isotope analysis

Chen

Bao

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND The high frequency and wide distribution of oil spills pose serious threats to the marine environmental ecosystem and human health. Microbial degradation is considered to be the most applicable means to process oil spills. Carbon isotope fractionation was employed as a crucial tool for evaluating the biodegradation of four crude oils by Rhodococcus sp. with Corexit 9500A as oil dispersant. RESULTS Following biodegradation, carbon isotope fractionation of n‐alkanes in four dispersed crude oil samples was measured by gas chromatography–isotope ratio mass spectrometry. A relationship between the carbon isotope ratios and residual concentrations of n‐alkanes was established based upon the Rayleigh equation, with a correlation coefficient, R2, ranging from 0.67 to 0.83. The carbon isotope enrichment factors for n‐alkanes of the four crude oil samples tended to be 0.04–0.25‰ in the residuals. The biodegradation level of n‐alkanes calculated from the Rayleigh equation agreed with the biodegradation results analyzed by gas chromatography–mass spectrometry. CONCLUSIONS The degree of microbial degradation was positively correlated to the carbon isotope fractionation in n‐alkanes. Compound‐specific stable isotope analysis is capable of quantifying the biodegradation level of crude oil or oil spills. © 2019 Society of Chemical Industry

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Effect of dispersants on the biodegradation of South Louisiana crude oil at 5 and 25 °C

Cited by 16 publications

References 18 publications

Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms

Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms

Acute toxicity of Corexit EC9500A and assessment of dioctyl sulfosuccinate as an indicator for monitoring four oil dispersants applied to diluted bitumen

Microbial degradation of four dispersed crude oils by Rhodococcus sp. evaluated using carbon stable isotope analysis

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