EFFECT OF DISSOLVED OXYGEN PARTIAL PRESSURE ON THE ACCUMULATION OF ASTAXANTHIN IN CHEMOSTAT CULTURES OF <i>HAEMATOCOCCUS LACUSTRIS</i> (CHLOROPHYTA)

Lee, Yuan‐Kun; Ding, Sun-Yeun

doi:10.1111/j.0022-3646.1995.00922.x

Cited by 17 publications

(5 citation statements)

References 8 publications

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“…4B). This value was higher than those reported by Lee and Ding (1995) Lee and Soh (1991) Under nitrate saturating conditions, the elemental composition of dry biomass (46.7% C, 8.0% N, 6.7% H) was fairly constant (Table I), the C/N ratio being close to 5.7. Under nitrate-limiting conditions, however, the nitrogen content of cells decreased significantly.…”

Section: Resultscontrasting

confidence: 58%

“…Accumulation of astaxanthin in H. pluvialis has been studied by a number of groups (Boussiba, 2000;Boussiba and Vonshak, 1991;Fábregas et al, 2001Fábregas et al, , 2003Harker et al, 1995;Kobayashi et al, 1991Kobayashi et al, , 1992aLee and Ding, 1995;Lee and Soh, 1991;Orosa et al, 2001;Zlotnik et al, 1993). The main general conclusions from these works are: (i) in order to induce astaxanthin accumulation, growth should be slowed-down or stopped; (ii) the astaxanthin accumulation rate increases with irradiance; and (iii) the kinetics of astaxanthin accumulation varies with the nature of the stress condition imposed.…”

Section: Introductionmentioning

confidence: 99%

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Efficient one‐step production of astaxanthin by the microalga Haematococcus pluvialis in continuous culture

Río

Acién

García-Malea

et al. 2005

Biotech & Bioengineering

101

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The performance of Haematococcus pluvialis in continuous photoautotrophic culture has been analyzed, especially from the viewpoint of astaxanthin production. To this end, chemostat cultures of Haematococcus pluvialis were carried out at constant light irradiance, 1,220 μE/m2 · s, and dilution rate, 0.9/d, but varying the nitrate concentration in the feed medium reaching the reactor, from 1.7 to 20.7 mM. Both growth and biomass composition were affected by the nitrate supply. With saturating nitrate, the biomass productivity was high, 1.2 g/L · d, but astaxanthin accumulation did not take place, the C/N ratio of the biomass being 5.7. Under moderate nitrate limitation, biomass productivity was decreased, as also did biomass concentration at steady state, whereas accumulation of astaxanthin developed and the C/N ratio of the biomass increased markedly. Astaxanthin accumulation took place in cells growing and dividing actively, and its extent was enhanced in response to the limitation in nitrate availability, with a recorded maximum for astaxanthin cellular level of 0.8% of dry biomass and of 5.6 mg/L · d for astaxanthin productivity. The viability of a significant continued generation of astaxanthin‐rich H. pluvialis cells becomes thus demonstrated, as also does the continuous culture option as an alternative to current procedures for the production of astaxanthin using this microalga. The intensive variable controlling the behavior of the system has been identified as the specific nitrate input, and a mathematical model developed that links growth rate with both irradiance and specific nitrate input. Moreover, a second model for astaxanthin accumulation, also as a function of irradiance and specific nitrate input, was derived. The latter model takes into account that accumulation of astaxanthin is only partially linked to growth, being besides inhibited by excess nitrate. Simulations performed fit experimental data and emphasize the contention that astaxanthin can be efficiently produced under continuous mode by adjustment of the specific nitrate input, predicting even higher values for astaxanthin productivity. The developed models represent a powerful tool for management of such an astaxanthin‐generating continuous process, and could allow the development of improved systems for the production of astaxanthin‐rich Haematococcus cells. © 2005 Wiley Periodicals, Inc.

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Section: Resultscontrasting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Efficient one‐step production of astaxanthin by the microalga Haematococcus pluvialis in continuous culture

Río

Acién

García-Malea

et al. 2005

Biotech & Bioengineering

101

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“…to irradiance and UV are mediated by the production of these molecules from oxygen generated during photosynthesis. Furthermore, high oxygen concentrations have been show to increase intracellular concentration of antioxidants such as astaxanthin in Haematococcus lacutris (Lee and Ding 1995) and result in increased antioxidant enzyme levels in Symbiodinium microadriaticum (Matta and Trench 1991). Other studies, however, have shown little effect of high oxygen tensions on a number of cultured microalgae.…”

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confidence: 99%

EFFECT OF HYPEROXIA ON THE GROWTH AND PHOTOSYNTHESIS OF POLAR SEA ICE MICROALGAE¹

McMinn

Pankowski

Delfatti

2005

Journal of Phycology

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Sea ice algal communities are naturally exposed to very high concentrations of dissolved oxygen, which are likely to lead to increasing stress levels and declines in productivity. To test this hypothesis, cultures of Fragilariopsis cylindrus (Grun?) Hasle, Pseudo-nitzschia sp., Fragilariopsis curta (Van Heurch), Porosira glacialis (Grunow), and Entomoneis kjellmannii (Cleve) from Antarctic sea ice and Nitzschia frigida from Arctic sea ice were exposed to elevated dissolved oxygen levels, and their growth, maximum quantum yield, relative maximum electron transport rate, and photosynthetic efficiency were measured. At oxygen concentrations equivalent to approximately four times air saturation (89% oxygen), the growth rate and maximum quantum yield were significantly reduced in all taxa. When the oxygen concentration was regularly allowed to drop, the effect on growth and quantum yield was reduced. At lower dissolved oxygen concentrations (52%), the declines in growth and quantum yield were reduced but were still mostly significantly different from the controls (21% oxygen). It is likely that the generation of excess active oxygen radicals in the presence of free oxygen is responsible for most of the decline in growth, maximum quantum yield, relative maximum electron transport rate, and photosynthetic efficiency in all species.Abbreviations: a, photosynthetic efficiency; dF/F m 0 , effective quantum yield; E k , minimum saturating irradiance; F v /F m , maximum quantum yield; NPQ, nonphotochemical quenching coefficient; rETR max , relative maximum electron transport rate; RLC, rapid light curve High dissolved oxygen concentrations under sea ice were first reported by McMinn et al. (2000). They noted that on bright sunny days, oxygen production by algae under the ice far exceeded its rate of removal by diffusion, leading to extensive oxygen bubble formation. Because of the increased solubility of oxygen in seawater at low temperatures, these organisms are likely to experience some of the highest dissolved oxygen concentrations on the planet, which is likely to be toxic to the sea ice algae. The question of how these algae cope with these high oxygen levels and other extreme conditions was also raised and discussed by Thomas and Dieckmann (2002). However, elevated dissolved oxygen concentrations are not unique to sea ice habitats but are found in the boundary layers of most microbial mat communities and frequently on the stapes or leaves of macrophytes and sea grasses. Oxygen concentrations as high as 560% air saturation, for instance, have been reported in boundary layers associated with seaweed stipes (Irwin and Davenport 2002), and similar dissolved oxygen concentrations have been observed above sediments dominated by diatoms (Raven 1991).Elevated oxygen levels are known to have an inhibitory effect on the metabolism and growth of plants (Raven 1991, Raven et al. 1994). There are two mechanisms thought to be largely responsible for these deleterious effects: the competitive effect of O 2 on RUBISCO (photorespiratio...

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“…The strains that exhibited the most varied results were CCM-UDEC 022 and CCM-UDEC 023. In fact, CCM-UDEC 022 showed the highest division rates (0.97 div day -1) and the highest cell densities (4.4 x 10 5 cells ml -1 ) under autotrophic conditions on day 14; both parameters were much higher than the normal range found in the literature for the species (0.5-0.7 div day -1 and 1.5-2.5 x 10 5 cell ml -1 , Hagen et al 1993, Lee & Ding 1994, 1995, Harker et al 1996, Orosa et al 2001. The CCM-UDEC 023 strain, in contrast, exhibited lower division rates than CCM-UDEC 022 (ca.…”

Section: Discussionmentioning

confidence: 58%

“…Although considerable information exists regarding the factors that promote growth (i.e. Kobayashi et al 1991, Barbera et al 1993, Zlotnik et al 1993, Fan et al1994, Lee & Ding 1995, Harker et al 1996, Gong & Chen 1997, Triphathi et al 1999, Hagen et al 2001, Orosa et al 2001, García-Malea et al 2005, Jeon et al 2006 and astaxanthin accumulation (review in Boussiba 2000, Domínguez-Bocanegra et al 2004, García-Malea et al 2005, Orosa et al 2005 in H. pluvialis, most of the studies have looked at single strains mainly from culture collections (i.e., UTEX, NIES, CCAP, ATCC, SAG, NIVA) and examined under a wide range of culture conditions.…”

Section: Introductionmentioning

confidence: 99%

Growth and Total Carotenoid Content in Four Chilean Strains of Haematococcus Pluvialis Flotow, Under Laboratory Conditions

2009

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Haematococcus pluvialis is a unicellular green microalga cultivated on a large scale as a natural source of astaxanthin, a ketocarotenoid of high commercial value due to its colorant and antioxidant properties. The aim of this study was to compare growth and total carotenoids among four strains of H. pluvialis isolated from different microhabitats in two geographical locations in Chile. Growth was carried out under autotrophic and mixotrophic conditions and under two photon flux densities (PFD) (20 and 85 µmol m-2 s-1). Total carotenoid content was estimated on day 45. Most of the strains grew better under the autotrophic conditions; the highest exponential growth rates were exhibited at the high PFD in all the strains (ranging from 0.73 to 1.20 div day-1) with the exception of CCM-UDEC 023 which showed the same exponential growth rate with either PFD (0.73 div day-1). However, the cell density estimated on day 14 was higher at the low PFD in CCM-UDEC 022 and CCM-UDEC 023 (4.4 x 10 5 and 8.9 x 10 4 cells ml-1 , respectively). The mixotrophic condition slightly improved the growth rate of the strain CCM-UDEC 021 at the high PFD (from 1.04 div day-1 at autotrophic condition to 1.25 and 1.14 div day-1 at 2mM and 10mM sodium acetate, respectively). The algal dry weight estimated on day 14 was not significantly correlated with the cell density, but it was, to a certain extent, related to the proportion and size of the different cell types quantified on that day. The strain with the highest dry weight at the end of the experimental period and under all the assayed conditions was CCM-UDEC 023. It was also the strain that produced the highest total carotenoid content under both autotrophic (5.6 mg l-1 and 95.6 pg cell-1 at the high PFD) and mixotrophic (with 2mM acetate) conditions (10.8 mg l-1 and 297 pg cell-1 at the high PFD). A high intraspecific variability in the physiological attributes analyzed existed among the strains under study. Even though the mixotrophic condition assayed did not substantially improve the growth rate of any strain, it did improve the total carotenoid yield in the strain CCM-UDEC 023.

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EFFECT OF DISSOLVED OXYGEN PARTIAL PRESSURE ON THE ACCUMULATION OF ASTAXANTHIN IN CHEMOSTAT CULTURES OF HAEMATOCOCCUS LACUSTRIS (CHLOROPHYTA)

Cited by 17 publications

References 8 publications

Efficient one‐step production of astaxanthin by the microalga Haematococcus pluvialis in continuous culture

Efficient one‐step production of astaxanthin by the microalga Haematococcus pluvialis in continuous culture

EFFECT OF HYPEROXIA ON THE GROWTH AND PHOTOSYNTHESIS OF POLAR SEA ICE MICROALGAE¹

Growth and Total Carotenoid Content in Four Chilean Strains of Haematococcus Pluvialis Flotow, Under Laboratory Conditions

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EFFECT OF DISSOLVED OXYGEN PARTIAL PRESSURE ON THE ACCUMULATION OF ASTAXANTHIN IN CHEMOSTAT CULTURES OF HAEMATOCOCCUS LACUSTRIS (CHLOROPHYTA)

Cited by 17 publications

References 8 publications

Efficient one‐step production of astaxanthin by the microalga Haematococcus pluvialis in continuous culture

Efficient one‐step production of astaxanthin by the microalga Haematococcus pluvialis in continuous culture

EFFECT OF HYPEROXIA ON THE GROWTH AND PHOTOSYNTHESIS OF POLAR SEA ICE MICROALGAE1

Growth and Total Carotenoid Content in Four Chilean Strains of Haematococcus Pluvialis Flotow, Under Laboratory Conditions

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EFFECT OF HYPEROXIA ON THE GROWTH AND PHOTOSYNTHESIS OF POLAR SEA ICE MICROALGAE¹