Effect of dissolved solids released from biochar on soil microbial metabolism

Hu, Jiajun; Han, Tao; Wang, Ya Zhu; Yang, Changzhu; Gao, Min-Tian; Tsang, Yiu Fai; Li, Jixiang

doi:10.1039/d2em00036a

Cited by 10 publications

(4 citation statements)

References 47 publications

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“…In this genus, some members were identified as N 2 fixers with a need of external organic C source, exhibiting a mixotrophic metabolism depending on the environmental conditions (J. Hu et al, 2022; Ryan et al, 2009). Sequences associated to the Rhodospirillaceae family were mainly affiliated to the species Azospirillum lipoferum which is able to grow in autotrophy with H 2 , CO 2 and N 2 (Tilak et al, 1986).…”

Section: Resultsmentioning

confidence: 99%

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Rous

Santa-Catalina

Quéméner

et al. 2023

Preprint

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The production of nitrogen fertilizers in modern agriculture is mostly based on the Haber-Bosch process, representing nearly 2% of the total energy consumed in the world. Low-energy bioelectrochemical fixation of N2to microbial biomass was previously observed on a biocathode but the microbial interactions in N2-fixing electroactive biofilms are still poorly understood. The present study aims to develop a method of enrichment of autotrophic and diazotrophic bacteria from soil samples for further characterization. The enrichment method was based on a first classical step of selection of N2-fixing bacteria from soil samples. Then, a polarized cathode was used for the enrichment of autotrophic bacteria using H2(hydrogenotrophic) or the cathode as energy sources. This enrichment was compared with an enrichment of diazotrophic hydrogenotrophic bacteria without the use of the microbial electrochemical system. Both methods showed comparable results for N2fixation rates at day 340 of the enrichment, with an estimated average of approximately 0.2 mgNfixed/L.d. Current densities up to -15 A/m² were observed in the polarized cathode enrichments and a significant increase of the microbial biomass on the cathode was shown between 132 and 214 days of enrichment. These results confirm the enrichment of autotrophic, electrotrophic and diazotrophic bacteria in the polarized cathode enrichments. Finally, the analysis of the enriched communities suggest thatDesulforamulus ruminismediated microbial interactions between autotrophic anaerobic and heterotrophic aerobic bacteria in polarized cathode enrichment. These interactions could play a key role in the development of biomass in these systems and on N2fixation. Based on these findings, a conceptual model on the functioning of mixed cultures N2-fixing electroactive biofilms was proposed.

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Section: Resultsmentioning

confidence: 99%

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Rous

Santa-Catalina

Quéméner

et al. 2023

Preprint

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show abstract

“…In this genus, some members were identified as N2 fixers with a need of external organic C source, exhibiting a mixotrophic metabolism depending on the environmental conditions (J. Hu et al, 2022;Ryan et al, 2009). Sequences associated to the Rhodospirillaceae family were mainly affiliated to the species Azospirillum lipoferum which is able to grow in autotrophy with H2, CO2 and N2 (Tilak et al, 1986).…”

Section: Microbial Communitiesmentioning

confidence: 99%

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Rous,

Santa-Catalina,

Desmond-Le Quémener

et al. 2024

Peer Community Journal

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The production of nitrogen fertilizers in modern agriculture is mostly based on the Haber-Bosch process, representing nearly 2% of the total energy consumed in the world. Lowenergy bioelectrochemical fixation of N 2 to microbial biomass was previously observed but the mechanisms of microbial interactions in N 2 -fixing electroactive biofilms are still poorly understood. The present study aims to develop a new method of enrichment of autotrophic and diazotrophic bacteria from soil samples with a better electron source availability than using H 2 alone. The enrichment method was based on a multi-stage procedure. The first enrichment step was specifically designed for the selection of N 2 -fixing bacteria from soil samples with organic C as electron and carbon source. Then, a polarized cathode was used for the enrichment of autotrophic bacteria using H 2 (hydrogenotrophic) or the cathode as electron source. This enrichment was compared with an enrichment culture of pure diazotrophic hydrogenotrophic bacteria without the use of a microbial electrochemical system. Interestingly, both methods showed comparable results for N 2 fixation rates at day 340 of the enrichment with an estimated average of approximately 0.2 mgN fixed /L.d. Current densities up to -15 A/m² were observed in the polarized cathode enrichments and a significant increase of the microbial biomass on the cathode was shown between 132 and 214 days of enrichment.These results confirmed an enrichment in autotrophic and diazotrophic bacteria on the polarized cathode. It was hypothesied that autotrophic bacteria were able to use either the H 2 produced at the cathode or directly the cathode through direct electron transfer (DET) as more biomass was produced than with H 2 alone. Finally, the analysis of the enriched communities suggested that Desulforamulus ruminis mediated microbial interactions between autotrophic anaerobic and heterotrophic aerobic bacteria in polarized cathode enrichment. These interactions could play a key role in the development of biomass in these systems and on N 2 fixation. Based on these findings, a conceptual model on the functioning of mixed cultures N 2 -fixing electroactive biofilms was proposed.

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“…Biochar provided protection for Sphingomonas.g that can utilize PAH as carbon source [32], thus promoting the degradation of PAH under the combined application of plants and biochar. The enrichment of Pseudoxanthomonas.g may be attributed to the enrichment of soil nutrients subsequent to biochar application [33], potentially leading to the promotion of NH4 + -N transformation and nitrogen fixation along with Ensifer.g (Table S3) [34,35]. The increase of soil PAH removal rate could be ascribed to root exudation promoting the degradation of soil PAH by Streptomyces.g [36].…”

Section: The Effects Of Biochar and Plant Roots On Pah Contaminated S...mentioning

confidence: 99%

The Combination of Buchloe dactyloides Engelm and Biochar Promotes the Remediation of Soil Contaminated with Polycyclic Aromatic Hydrocarbons

Wang,

Li,

Zou

et al. 2024

Microorganisms

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Polycyclic aromatic hydrocarbons (PAHs) cause serious stress to biological health and the soil environment as persistent pollutants. Despite the wide use of biochar in promoting soil improvement, the mechanism of biochar removing soil PAHs through rhizosphere effect in the process of phytoremediation remain uncertain. In this study, the regulation of soil niche and microbial degradation strategies under plants and biochar were explored by analyzing the effects of plants and biochar on microbial community composition, soil metabolism and enzyme activity in the process of PAH degradation. The combination of plants and biochar significantly increased the removal of phenanthrene (6.10%), pyrene (11.50%), benzo[a]pyrene (106.02%) and PAHs (27.10%) when compared with natural attenuation, and significantly increased the removal of benzo[a]pyrene (34.51%) and PAHs (5.96%) when compared with phytoremediation. Compared with phytoremediation, the combination of plants and biochar significantly increased soil nutrient availability, enhanced soil enzyme activity (urease and catalase), improved soil microbial carbon metabolism and amino acid metabolism, thereby benefiting microbial resistance to PAH stress. In addition, the activity of soil enzymes (dehydrogenase, polyphenol oxidase and laccase) and the expression of genes involved in the degradation and microorganisms (streptomyces, curvularia, mortierella and acremonium) were up-regulated through the combined action of plants and biochar. In view of the aforementioned results, the combined application of plants and biochar can enhance the degradation of PAHs and alleviate the stress of PAH on soil microorganisms.

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Effect of dissolved solids released from biochar on soil microbial metabolism

Abstract: Dissolved solids released from biochar (DSRB), including organic and inorganic compounds, may affect the role of biochar as a soil amendment. In this study, the effects of DSRB on soil...

Cited by 10 publications

References 47 publications

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

Comparison of enrichment methods for efficient nitrogen fixation on a biocathode

The Combination of Buchloe dactyloides Engelm and Biochar Promotes the Remediation of Soil Contaminated with Polycyclic Aromatic Hydrocarbons

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