Effect of docosahexaenoic acid (DHA) on arachidonic acid metabolism and platelet function

Rao, Gundu H. R.; Radha, E.; White, James G.

doi:10.1016/0006-291x(83)91235-4

Cited by 79 publications

(21 citation statements)

References 26 publications

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“…Finally, the overall marker of lipid peroxidation, the isoprostane 8-epi-PGF 2α in urine, was significantly lower and higher after 200 mg/day and 1600 mg/day, respectively (20). The decrease of the generated leukotriene (LT) B 4 in calcium ionophore-stimulated PMNs was compensated by LTB 5 , which is known to be much less proinflammatory than LTB 4 (23), and this was proportional to the intake of DHA (24). Altogether, these results indicate that, except for PMNs, cell functions were mostly affected after 400 and 800 mg/day.…”

Section: Dose-effect Of Dha Intake On Blood Cells and Low-density Lipmentioning

confidence: 98%

“…This could be due to structural effects, in relation to the folding of the molecule induced by its six cis/Z double bonds (4). Another possibility is to inhibit the conversion of arachidonic acid (ARA) at the level of the cyclooxygenase enzymes (5), then decreasing the formation of the atherothrombotic agent thromboxane (Tx) A 2 from ARA. In addition, DHA is easily oxygenated by lipoxygenases (6,7) and some of their end-products may counteract the effect of TxA 2 on its receptor site (8).…”

Section: Introductionmentioning

confidence: 99%

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Dose-effect and metabolism of docosahexaenoic acid: Pathophysiological relevance in blood platelets

Lagarde

Calzada

Guichardant

et al. 2013

Prostaglandins, Leukotrienes and Essential Fatty Acids

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Docosahexaenoic acid (DHA) is known as a major nutrient from marine origin. Considering its beneficial effect in vascular risk prevention, the effect of DHA on blood components, especially platelets, will be reviewed here. Investigating the dose-effect of DHA in humans shows that daily intake lower than one gram/day brings several benefits, such as inhibition of platelet aggregation, resistance of monocytes against apoptosis, and reinforced antioxidant status in platelets and lowdensity lipoproteins. However, higher daily intake may be less efficient on those parameters, especially by losing the antioxidant effect. On the other hand, a focus on the inhibition of platelet aggregation by lipoxygenase end-products of DHA is made. The easy conversion of DHA by lipoxygenases and the formation of a double lipoxygenation product named protectin DX, reveal an original way for DHA to contribute in platelet inhibition through both the cyclooxygenase inhibition and the antagonism of thromboxane A 2 action.

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Section: Dose-effect Of Dha Intake On Blood Cells and Low-density Lipmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Dose-effect and metabolism of docosahexaenoic acid: Pathophysiological relevance in blood platelets

Lagarde

Calzada

Guichardant

et al. 2013

Prostaglandins, Leukotrienes and Essential Fatty Acids

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“…In preparations of intact human platelets and of ram seminal vesicle microsomal-derived prostaglandin synthetase, both EPA and DCHA are poor substrates and compete with AA for conversion by cyclooxygenase (15)(16)(17). Furthermore, the prostaglandin endoperoxides and thromboxane A3 derived The mixtures were incubated at 37°C for 5 min, and the reactions were stopped by rapid cooling at 4°C and centrifugation at 10,000 g for 30 s. 50 Ml of the supernatant from each sample was added to 10 ml scintillation fluid and the radioactivity was measured.…”

Section: Introductionmentioning

confidence: 99%

Effects of exogenous arachidonic, eicosapentaenoic, and docosahexaenoic acids on the generation of 5-lipoxygenase pathway products by ionophore-activated human neutrophils.

Lee¹,

Mencia‐Huerta²,

Shih³

et al. 1984

J. Clin. Invest.

252

122

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Abstract. Exogenous eicosapentaenoic acid (EPA) and docosahexaenoic acid (DCHA) have been compared with exogenous arachidonic acid for their capacity to modulate the oxidative metabolism of membrane-derived arachidonic acid by the 5-lipoxygenase pathway in ionophore-activated human neutrophils and for their suitability as parallel substrates in this pathway. The products from specific 14C-or 3H-labeled substrates were isolated by reverse phase high performance liquid chromatography (RP-HPLC) and were identified by elution of radiolabel at the retention times of the appropriate synthetic standards. Each product was also characterized by its ultraviolet (UV) absorption spectrum, and 7-hydroxy-DCHA was defined in addition by analysis of its mass spectrum. The metabolites, 5-hydroxyeicosatetraenoic acid, leukotriene B4 (LTB4), 6-trans-LTB4 diastereoisomers, 5-hydroxyeicosapentaenoic acid, 6-trans-leukotriene B5 diastereoisomers, leukotriene B5 (LTB5), and 7-hydroxy-DCHA were quantitated by integrated UV absorbance during resolution by RP-HPLC. LTB4 and LTB5 were also quantitated by

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“…The oxidation of DHA is not produced by cyclooxygenase. DHA does competitively inhibit the activity of this enzyme, as well as the phospholipases A2 and C, which catalyze the release of FA from membrane phospho lipids [17,18].…”

Section: Fa Composition O F Plasma Lipidsmentioning

confidence: 99%

Influence of a Single Parenteral Application of a 10&percnt; Fish Oil Emulsion on Plasma Fatty Acid Pattern and the Function of Thrombocytes in Young Adult Men

Elmadfa¹,

Ströh²,

Brandt³

et al. 1993

Ann Nutr Metab

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50 ml of a 10% fish oil emulsion (41% ω-3 fatty acids of total fatty acids) were infused for 1 h into the arm vein of young, healthy, male volunteers. The fatty acid composition of the plasma, aggregation of the blood platelets as well as the thromboxane synthesis, were measured before the beginning of infusion, 20, 60, 120, 360 and 1,440 min after the start of the fat infusion. In the first 60 min, the fatty acid composition of the plasma changed in correspondence with the supplied fatty acid pattern. At the end of the investigation it was again within the normal range. As a result of fat application thromboxane synthesis was reduced and the aggregation of the platelets was inhibited but it was normalized by the 1,440-min value. Fish oil emulsions might be beneficial for parenterally fed patients with a high risk of thrombosis. Therefore the performance of further investigations using a varying dosage and multiple application can be recommended.

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Effect of docosahexaenoic acid (DHA) on arachidonic acid metabolism and platelet function

Cited by 79 publications

References 26 publications

Dose-effect and metabolism of docosahexaenoic acid: Pathophysiological relevance in blood platelets

Dose-effect and metabolism of docosahexaenoic acid: Pathophysiological relevance in blood platelets

Effects of exogenous arachidonic, eicosapentaenoic, and docosahexaenoic acids on the generation of 5-lipoxygenase pathway products by ionophore-activated human neutrophils.

Influence of a Single Parenteral Application of a 10&percnt; Fish Oil Emulsion on Plasma Fatty Acid Pattern and the Function of Thrombocytes in Young Adult Men

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