Effect of donor specific transfusion (DST) is restricted to the rat combination used and closely related with alloantibody in heart transplantation

Abstract

SummaryThe effect of donor specific transfusion (DST) in several donor-recipient combinations was examined using the rat heart transplantation model. In some donor-recipient combinations such as LEW to DA, LEW to ACI, LEW to PVG and PVG to DA, DST pretreatment could induce permanent graft survival (> 100 days). But in DA to LEW, ACI to LEW, LEW to BN, BN to LEW, PVG to LEW and BN to DA combinations DST pretreatment had no effect on graft survival. The effect of pre-immunization with several DA antigens (heart, liver, lymph node cells and red blood cells) was examined using the DA to LEW combination in which DST pretreatment was not effective. In no case was permanent graft survival obtained. Alloantibody levels (anti-class I and anti-class I + II, respectively) were analysed at the time of heart transplantation and in the post-transplantation period. The anti-class I antibody levels were low or not detected in rats with surviving grafts, but in the rats which failed to have the prolonged graft survival, anti-class I antibody levels were high. This study showed that the effect of DST was restricted to particular donor-recipient rat combination in the heart transplantation model.

Development of Infectious Tolerance After Donor-Specific Transfusion and Rat Heart Transplantation

“…Donor-specific transfusions (DST) 3 induce both experimental and clinical donor-specific allograft tolerance (15,18,19,(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34).…”

Intestinal allograft survival in the rat following pretreatment with donor-specific UV-B-irradiated leukocytes and peritransplant immunosuppression with cyclosporine

“…In conclusion, it appears that induction of immunologic unresponsiveness requires strict attention to (a) the antigen dosage [25], (b) the specificity of the cell type used for pretreatment, (c) the timing of immunization, (d) the route of antigen administration, (e) the responder status of the host, and (f) the rat combination used [8]. In addition, the prolongation that follows DST appears to be influenced by the specific composition of tissue transplanted, since intestinal allografts are rejected, while cardiac allografts in the same rat strain combination survive indefinitely when the recipients are subjected to the same pretreatment protocol using W-B-irradiated DL and a brief course of peritransplant cyclosporine.…”

The effect of intrathymic injection of donor blood on the graft versus host reaction and cardiac allograft survival in the rat

“…Our laboratory as well as others have shown that antigen pretreatment can prolong graft survival in rodent models and that the effect is donor specific (1)(2)(3)(4). The mechanism, however, by which pretreatment of recipients with donor antigen results in prolonged graft survival is not known (5).…”

“…In the rat heart transplantation model the effectiveness of donor specific transfusion (DST) is related to the donor-recipient strain combination. In particular, it is difficult to induce tolerance in Lewis rat recipients while pretreatment with DST quite readily results in prolonged allograft survival in the DA and PVG strains (1). In this report, a new approach to induce the tolerance using pretreatment with donor antigen in Lewis rat recipients was tested.…”

“…The only previous report on the effect of DST in GVHR models documented the ability of intravenous whole blood to suppress the GVHR (8). We investigated the effects of DST in GVHR models; in a successful DST rat strain (DA, PVG rats) and in an unsuccessful DST rat strain (Lewis rat) (1). As well, we examined the effect of intrathymic injection of donor blood or bone marrow cells on lymphocyte function in the GVHR model.…”