Effect of Doublesynch and Estradoublesynch protocols on estrus induction, conception rate, plasma progesterone, protein, and cholesterol profile in anestrus Gir heifers

Abstract

AimThis study aimed to evaluate the efficacy of Doublesynch and Estradoublesynch protocols on estrus induction, conception rates, plasma progesterone, protein, and cholesterol profile in anestrus Gir heifers.Materials and MethodsIn this study, 50 pubertal anestrus Gir heifers were selected from the field and farm conditions. The heifers were dewormed (injection ivermectin, 100 mg, s/c) and supplemented with minerals and vitamins (injection organic phosphorus 800 mg and injection Vitamin AD3E and Biotin 10 ml i/m) and multi-mineral bolus at 1 bolus daily for 7 days. The heifers were randomly divided into three groups: Doublesynch (n=20), Estradoublesynch (n=20), and control (n=10). The animals were monitored for estrus response, estrus interval, behavioral signs, and conception rates after induced/first, second, and third cycle post-treatment. Blood samples were obtained on day 0, day 9, day 12, and on day 12 post-artificial insemination (AI) for determination of plasma progesterone, protein, and cholesterol profile.ResultsThe estrus response rate between Doublesynch and Estradoublesynch protocols was similar between treated heifers (85% and 95%). The interval from the second prostaglandin F2α (PGF2α) injection to estrus induction did not differ between the groups (63.87±4.19 vs. 58.27±3.83 h). The conception rates following induced estrus (20% vs. 30%), at the second cycle (23.07% vs. 16.66%), at the third cycle (22.22% vs. 30.00%), and the overall conception rate (45% and 55%) within 27.89±5.75 and 26.45±5.48 days were the same across the treatment groups. The mean plasma progesterone concentrations were significantly (p<0.01) higher on day 9 (second PGF2α injection) and day 12 post-AI compared to day 0 (first PGF2α injection) and the day of fixed-timed artificial insemination. The concentrations were also significantly (p<0.05) higher in conceived than non-conceived heifers on day 9 of treatment and day 12 post-AI in both the protocols. The mean plasma cholesterol concentrations were significantly higher during peak follicular and luteal phases compared to the initial anestrus phase in both the protocols. The values were also higher in non-conceived than conceived animals in both the protocols. The plasma protein profile was not influenced by the sampling days or conceived and non-conceived status.ConclusionThe results showed that both Doublesynch and Estradoublesynch protocols resulted in similar estrus induction and conception rates with modulation of plasma progesterone and cholesterol profile in anestrus Gir heifers.

Evaluation of Double Ovsynch Protocol in Acyclic Jaffarabadi Heifers and Buffaloes with Respect to Ovarian Dynamics, Hormonal Profile and Fertility

“…These findings with respect to values coincided well with the previous reports of Singh et al (2004), Jerome et al (2016) and Prajapati et al (2018). However, other reserchers (Parmar et al, 2012;Chaudhary et al, 2018) reported higher values of total protein than the present findings. Optimum protein level is necessary for the development of endocrine and sex organs.…”

Evaluation of kisspeptin and ovsynch protocol in the treatment of postpartum anoestrus cattle with respect to intensity and ovulation rate

“…(2013) [1] also reported similar observation in anoestrous Kankrej cows treated with Ovsynch protocol while higher intensity of oestrus (33.33-58.33%) than that of the present investigation was reported by Chaudhari et al (2012) [6] and Nevkar et al (2012) [14] in acyclic crossbred cows treated with Ovsynch protocol. Velladurai et al (2014) [25] recorded the intensity of oestrus as intense, intermediate and weak in 50.00, 37.50 and 12.50 percent cows treated with Ovsynch protocol which was found higher than that recorded in the present investigation.…”

Changes in ovarian structures, blood biochemical and hormonal profile in double Ovsynch treated conceived and non-conceived Jaffarabadi buffaloes

“…Cholesterol serves as a precursor for the synthesis of progesterone by ovarian luteal cells. In a recent study, significantly higher mean plasma cholesterol concentration was documented in non-conceived than conceived Gir heifers treated with Doublesynch and Estradoublesynch protocols (Chaudhary et al 2018). Moreover, the mean plasma protein levels on the day of FTAI and day 12 post-AI were significantly higher compared to day 0 and 9 of the Estradoublesynch protocol.…”