Effect of dry heat treatment on seed-borne<i>Fusarium graminearum</i>and other cereal pathogens

Clear, R. M.; Patrick, Susan K.; Wallis, Robert; Turkington, T. K.

doi:10.1080/07060660209507038

Cited by 28 publications

(19 citation statements)

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“…The agar method is also more suitable in detecting viability of the pathogens after thermal treatment (Clear et al 2002) or spraying with a fungicide. However, PCR-based tests are preferable to conventional methods for screening for quarantine pathogens, to estimate the risk of mycotoxin contamination by toxigenic Fusarium species and for the assessment of fungal mass (Waalwijk et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Real-Time PCR and Agar Plating Method to Predict Fusarium Verticillioides and Fumonisin B1 Content in Nigerian Maize

Adejumo¹,

Hettwer²,

Nutz³

et al. 2009

Journal of Plant Protection Research

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Eighty maize grain samples collected in Nigeria were investigated for fumonisin B 1 (FB 1 ) content and Fusarium verticillioides colonization. F. verticillioides DNA was quantified by species-specific real-time PCR and living propagules of the fungus were counted by agar-plating method. FB 1 was detected in 55 (68.7%) of the total samples (mean: 98.5 µg/kg, range: 10 to 714 µg/kg) at 10 µg/kg detection limit. The mean amount of F. verticillioides DNA determined by real-time PCR was 49.7 µg/kg (range: 10-126.7 µg/kg), while agar plate method showed the presence of F. verticillioides in 45 samples (mean incidence: 21.0%, range: 6.7-60.0%). There was correlation ties between F. verticillioides DNA by real time PCR and fungal colonization by agar plate method (R = 0.71, p = 00001 at 95% confidence level), and means of FB 1 and F. verticillioides DNA in the yellow and white maize were significantly different. Despite the high consumption of maize in Nigeria, the amount of FB 1 ingested by consumers appears to be low. The estimated daily intake of fumonisins was 0.21 µg/kg body weight per day.

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Section: Discussionmentioning

confidence: 99%

Real-Time PCR and Agar Plating Method to Predict Fusarium Verticillioides and Fumonisin B1 Content in Nigerian Maize

Adejumo¹,

Hettwer²,

Nutz³

et al. 2009

Journal of Plant Protection Research

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“…Much research around the world has been carried out on thermal seed treatments resulting in superior control of the incidence of pathogens (Daniels, 1983;Clear et al, 2002;Coutinho et al, 2007;Bennett and Colyer, 2010). Clear et al (2002) evaluate the incidence of F. graminearum and the viability of wheat and barley seeds after treatment with dry heat at 50°C, 70°C, 60°C and 80°C. The authors find that dry heat treatments can eliminate F. graminearum completely from wheat seeds without a significant reduction of seed germination under treatments at 70°C and 80°C.…”

Section: Introductionmentioning

confidence: 99%

Effect of thermal and in vitro fungicide treatments on pathogens of the genus Fusarium associated with maize seeds

2020

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Stalk rot in maize plants is commonly associated with manyspecies of the genus Fusarium. This disease affects the seedbedand the establishment of maize crops because of seedscontaminated with different pathogens of this genus. Maizecrops in the Ubate Valley, in the province of Cundinamarca,are currently infected by this disease, which reduces the yieldand final quality of the maize seeds. This research evaluatedthe effects of thermal and fungicide treatments on pathogensof the genus Fusarium associated with maize seeds. Seeds weretreated at 50°C, 55°C and 60°C with dry heat and hot water. Mycelial colonization of seeds, germination percentage, seedling length, and fresh weight were evaluated as variables. In in vitro tests, the fungicides fludioxonil + metalaxyl-M, tebuconazole + trifloxystrobin, prochloraz + difenoconazole and carboxin + captan were evaluated at 0.5, 1.0 and 1.5 the commercial dose on the radial growth and conidial germination of Fusarium subglutinans and Fusarium graminearum isolates. The most effective heat treatments on Fusarium colonization of maize seeds were obtained with the two heat sources at 55°C without a significant reduction in the percentage of germination and seedling length and fresh weight. Commercial doses of theevaluated fungicides completely inhibited the radial growthof F. graminearum and only commercial doses of carboxin +captan and prochloraz + difenoconazole completely inhibited F. subglutinans growth. Germination inhibition of F. subglutinansand F. graminearum conidia was found with fludioxonil + metalaxyl-M at the three evaluated doses.

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“…Cleaned and dried seeds were filled on the steel container and kept on the hot air oven at 70 ∘ C for 2 days to sterilize the seed and make it free from pathogens and weevils. The pathogen was eliminated from maize seeds treated at 60 ∘ C for 15 days or at 70 ∘ C for 2 days and germination rates in most samples were not affected [20].…”

Section: Introductionmentioning

confidence: 99%

Role of Maize Weevil, Sitophilus zeamais Motsch. on Spread of Aspergillus section flavi in Different Nepalese Maize Varieties

Bhusal¹,

Khanal²

2019

Advances in Agriculture

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Experiments were conducted to find out the role of maize weevil, Sitophilus zeamais Motsch. on spread of green fungus, Aspergillus section flavi, in different varieties of stored maize in laboratory in 2016. Lab experiment was conducted to find the role of weevil on spread of A. flavus on five main varieties of maize grown at Nepal in split plot design, namely, Arun-2, Arun-4, Manakamana-1, Manakamana-3, and Rampur composite with three replications at NAST, Khumaltar, from August to September 2016. One hundred grams of each maize variety was exposed to weevil along with fungus and with fungus only to see the spread of the fungus under presence and absence of weevil. Among the tested five maize varieties, the lowest infestation was observed on Rampur Composite (14.99%) while it was the highest on Manakamana-3 (87.70%). The highest mean infestation (75.58%) was found under weevil released condition while it was lower (62.16%) under nonreleased condition. In presence of weevil, the infestation of the fungus increased and in their absence the infestation was low which signifies the role of weevil in fungal spread. All indices indicate that Rampur composite is the best variety among the five tested varieties in terms of storage under the presence of fungus and weevils. This study also indicates ample scope for further study on different varieties of maize under several storage conditions.

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Effect of dry heat treatment on seed-borneFusarium graminearumand other cereal pathogens

Abstract: Fungal nail infections are common and notoriously difficult to treat. In this paper I present two cases of fungal nail infection treated conservatively with dry heat using a regular electromechanical blowdryer (hair dryer).

Cited by 28 publications

References 10 publications

Real-Time PCR and Agar Plating Method to Predict Fusarium Verticillioides and Fumonisin B1 Content in Nigerian Maize

Real-Time PCR and Agar Plating Method to Predict Fusarium Verticillioides and Fumonisin B1 Content in Nigerian Maize

Effect of thermal and in vitro fungicide treatments on pathogens of the genus Fusarium associated with maize seeds

Role of Maize Weevil, Sitophilus zeamais Motsch. on Spread of Aspergillus section flavi in Different Nepalese Maize Varieties

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