Effect of Dust on the Viability of Vibrio fischeri in the Microtox Test

Park, Keummi; Hee, Shane S. Que

doi:10.1006/eesa.2001.2109

Cited by 16 publications

(10 citation statements)

References 34 publications

(35 reference statements)

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“…Compared to the test using Daphnia magna, Microtox ® has advantages of short time for the test preparation (cultivation and reconstitution of bacteria) and relatively easier test method. Thus Microtox ® has been used as a standard government eco-toxicological test method in a number of countries (i.e., Canada, The Netherlands, France, Germany, Sweden, and Spain) (Keddy et al 1995;Park et al 2001). Microtox ® Bioassay is a method originally designed for aqueous samples, so it has been reported to have relatively high accuracy and reliability for liquid samples test.…”

Section: Introductionmentioning

confidence: 99%

“…Burga-Perez et al (2012) Phase Assay or MLPA) based on different intervals and methods of filtration to get rid of the turbidity effect (Burga Perez et al 2012. Owing to these shortcomings of solid-phase toxicity test methods, many researchers have tested the solid particles regardless of their toxic effects, after completely separating the solid particles from the sample using centrifugation or filtration (Dombroski et al 1996;Ringwood et al 1997;Romkens et al 1999;Harkey et al 2000;Park et al 2001;Tiensing et al 2001;Jung et al 2001;Loureiro et al 2005;Parvez et al 2006). However, removal of the particles by those methods (leachate-basic test, L-BT) may cause an underestimation of total toxicity, if the particles removed have a toxic load.…”

Section: Introductionmentioning

confidence: 99%

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Proposed method for controlling turbid particles in solid-phase bioluminescent toxicity measurement

Yeo

Park

Ahn

et al. 2015

Environ Monit Assess

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In the recent half century, numerous methods have been developed to assess ecological toxicity. However, the presence of solid-particle turbidity sometimes causes such tests to end with questionable results. Many researchers focused on controlling this arbitrary turbidity effect when using the Microtox® Solid-Phase toxicity system, but there is not yet a standard method. In this study, I examined four solid-phase sample test methods recommended in the Microtox® manual, or proposed from the literature, and compared the existing methods with the proposed method (c-BSPT, centrifuged-BSPT). Four existing methods use the following strategies to control turbid particles: complete separation of liquid and solid using 0.45-μm filtration before contacting solid samples and bacteria, natural settlement, moderate separation of large particles using coarser-pore-size filtration, and exclusion of light loss in the toxicity calculation caused by turbidity after full disturbance of samples. The proposed method uses moderate centrifugation to separate out the heavier soil particles from the lighter bacteria after direct ii contact between them. Among the solid-phase methods tested, in which the bacteria and solid particles were in direct contact (i.e., three existing methods and the newly proposed one, c-BSPT), no single method could be recommended as optimal for samples over a range of turbidity. Instead, a simple screening strategy for selecting a sample-dependent solid-phase test method was suggested, depending on the turbidity of the solid suspension.The results of this study highlight the importance of considering solid particles, and the necessity for optimal selection of test method to reduce errors in the measurement of solid-phase toxicity.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proposed method for controlling turbid particles in solid-phase bioluminescent toxicity measurement

Yeo

Park

Ahn

et al. 2015

Environ Monit Assess

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“…Considering different analytical procedures, the Basic Solid Phase Test (Azur Environmental 1998) corresponds to the toxicity of the entire sediment and has been commonly applied for marine sediments (Salizzato et al 1998, Lahr et al 2003, Vigano et al 2003, Coz et al 2008. It has also been approved as a standard government ecotoxicological bioassay in several countries like Canada, The Netherlands, France, Germany, Sweden and Spain (Keddy et al 1995, Park & Hee 2001. Generally, the most relevant advantage of toxicity tests over the instrumental analysis, where the extrapolation of uncertain completeness is required, is their direct appreciation of the biological availability and the impact.…”

Section: Introductionmentioning

confidence: 99%

Geostatistical methods for estimation of toxicity of marine bottom sediments based on the Gdańsk Basin area

Witt

Kobusińska

Maciak

et al. 2014

Oceanological and Hydrobiological Studies

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Toxicity assessment of environmental compartments, in particular sediments as a highly complex matrix, provides a more direct way to assess potential adverse effects of pollutants present in a sample in contrast to chemical analysis estimating only a quantitative level of xenobiotics. Interactions between chemicals, formations of derivatives and the influence of chemical properties of sediments such as the organic matter content causing the intensified sorption of hydrophobic pollutants suggest that a traditional approach to the sediment quality, based only on chemical analysis may be insufficient. The presented study describes the vertical and horizontal variability of toxicity of Gdańsk Basin sediments. Based on 128 surface sediments samples and using geostatistical methods, a prediction map for the EC50 parameter was created. This allowed the evaluation of the toxicity of the surface sediment layer at any selected point of the study area. The applied analysis can be functional for many other locations worldwide. In the present study, the hypothesis about the location of toxic sediments in the vicinity of Gdańsk Deep, outer Puck Bay and close to Vistula River mouth was further confirmed.

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“…Also, bacterial enzyme assays such as Microtox® (Al-Mutairi et al, 2008) and LumiStox® and genotoxicity tests such as the Ames test (Beâ-kaert et al, 1999), SOS chromotest (Bombardier et al, 2001) and Vitotox® (Park and Que Hee, 2001) are used to monitor toxicity levels.…”

Section: Introductionmentioning

confidence: 99%

RESEARCH PAPER Phytotests as tools for monitoring the bioremediation process of soil contaminated with diesel oil

Wieczorek¹,

Kwapisz²,

Marchut-Mikołajczyk³

et al. 2012

bta

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The aim of the study was to determine changes in phytotoxicity levels during bioremediation of soil contaminated with 5% (v/w) diesel oil and find their correlation with the effectiveness of hydrocarbon degradation. Bioremediation trials were performed using a Gordonia alkanivorans S7 strain. The clean-up process was enhanced through the addition of dried fungal enzymatic preparations obtained from fungi Phanerohaete chrysosporium and Aspergillus niger. After 110 days of bioremediation the decrease in soil pollution ranged between 68 and 77% depending on treatment conditions. Toxicological tests using marker plants revealed significant differences in the phytotoxicity levels of soil during bioremediation, depending on the treatment variant. The addition of an enzymatic fungal preparation to soil was found to accelerate the rate of contaminant degradation. The rate of hydrocarbon depletion in subsequent phases of the remediation process was found to be correlated with the phytotoxicity level. The obtained results provide evidence that phytotests can be useful tools for monitoring toxicity changes during bioremediation either mediated only by bacteria or additionally stimulated by fungal enzymatic preparations.

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Effect of Dust on the Viability of Vibrio fischeri in the Microtox Test

Cited by 16 publications

References 34 publications

Proposed method for controlling turbid particles in solid-phase bioluminescent toxicity measurement

Proposed method for controlling turbid particles in solid-phase bioluminescent toxicity measurement

Geostatistical methods for estimation of toxicity of marine bottom sediments based on the Gdańsk Basin area

RESEARCH PAPER Phytotests as tools for monitoring the bioremediation process of soil contaminated with diesel oil

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