Under microgravity conditions, it is drown attention that the three-dimensional distribution of dust particles density in complex plasmas. Based on the three-dimensional hydrodynamic simulation, the influence of different coupling parameters, shielding parameters, charge of dust particles and plasma density on the Mach cone by laser-induced is studied in complex plasmas under microgravity conditions. When the shielding parameters are large, it is found that three different formulas of coupling parameters [20], [27] and [23] have a big impact on the disturbance density of dust particles, and the simulation results more agree with the theoretical expectations under the third formulas. In addition, when the laser radiation force is parallel or vertical to the laser movement speed, the Mach cone structure is symmetrical or antisymmetric in the three-dimensional space, which is mainly based on the asymmetry of the laser disturbance mode. Besides, increasing the shielding parameters, or reducing the charge of dust particles, or reducing the plasma density all will enhance the shielding interaction between the dust particles, further making the Mach cone formed by the dust disturbance density more localized around the laser spot, characterized by a narrowing of the disturbance range and an increasing density value. It is expected that this work can provide some reference for the theoretical and experimental studies of laser-induced Mach cone in three-dimensional complex plasma under microgravity conditions.