Effect of eCG treatment on gene expression of selected matrix metalloproteinases (MMP-2, MMP-7, MMP-9, MMP-10, and MMP-13) and the tissue inhibitors of metalloproteinases (TIMP-2 and TIMP-3) in the chicken ovary

Wolak, Dominika; Sechman, Andrzej; Hrabia, Anna

doi:10.1016/j.anireprosci.2020.106666

Cited by 19 publications

(11 citation statements)

References 34 publications

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“…In follicles of the mature hen ovary, the expression of MMP-2, MMP-3, MMP-9, MMP-10, and MMP-13 mRNAs and/or proteins as well as gelatinase (MMP-2 and MMP-9) activities increase along with follicular maturation [ 36 , 38 , 39 , 40 , 41 , 42 , 43 ]. These correlative findings suggest the engagement of some MMPs in the turnover of the ECM/follicular wall structure during the final stage of ovarian follicle maturation.…”

Section: Mmp Roles In Follicle Development and Atresiamentioning

confidence: 99%

“…Importantly, all the MMPs detected are expressed more strongly in the theca than in the granulosa layer. In the latter, MMPs are at a very low level, barely detectable (e.g., MMP-9, MMP-13) or not detectable (e.g., MMP-7) [ 38 , 39 , 40 , 41 , 42 , 43 ]. These findings are consistent with the presence of fibroblasts, which are a known source of MMPs in the theca layer.…”

Section: Mmp Roles In Follicle Development and Atresiamentioning

confidence: 99%

“…Gonadotropins seem to be important regulators of MMP system proteins in the ovarian follicles of birds. Recently, the potential engagement of gonadotropins in the regulation of expression and activity of several MMPs as well as TIMP expression in ovarian follicles at different stages of development were evaluated in vivo in chickens [ 43 ]. For this purpose, laying hens were treated for several days with equine chorionic gonadotropin (eCG) which exerts a primarily FSH-like bioactivity in birds and has a stimulatory impact on follicle selection and growth of recruited follicles.…”

Section: Mmp Regulation In the Avian Reproductive Systemmentioning

confidence: 99%

“…Based on these observations, it has been suggested that gonadotropins, principally FSH, regulate transcription, translation, and/or activity of MMP system constituents. In turn, these MMPs and TIMPs participate in the mechanisms that underlie ECM remodeling and cell function throughout ovarian follicle development in the chicken ovary [ 43 ]. As described in chapter 6, the LH may primarily stimulate the synthesis/activity of MMPs leading to follicle rupture during ovulation [ 53 ].…”

Section: Mmp Regulation In the Avian Reproductive Systemmentioning

confidence: 99%

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Matrix Metalloproteinases (MMPs) and Inhibitors of MMPs in the Avian Reproductive System: An Overview

Hrabia

2021

IJMS

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Many matrix metalloproteinases (MMPs) are produced in the mammalian reproductive system and participate in the regulation of its functions. In birds, the limited information available thus far indicates that MMPs are significant regulators of avian ovarian and oviductal functions, too. Some MMPs and inhibitors of MMPs are present in the hen reproductive tissues and their abundances and/or activities change according to the physiological state. The intraovarian role of MMPs likely includes the remodeling of the extracellular matrix (ECM) during folliculogenesis, follicle atresia, and postovulatory regression. In the oviduct, MMPs are also involved in ECM turnover during oviduct development and regression. This study provides a review of the current knowledge on the presence, activity, and regulation of MMPs in the female reproductive system of birds.

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Section: Mmp Roles In Follicle Development and Atresiamentioning

confidence: 99%

Section: Mmp Roles In Follicle Development and Atresiamentioning

confidence: 99%

Section: Mmp Regulation In the Avian Reproductive Systemmentioning

confidence: 99%

Section: Mmp Regulation In the Avian Reproductive Systemmentioning

confidence: 99%

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Matrix Metalloproteinases (MMPs) and Inhibitors of MMPs in the Avian Reproductive System: An Overview

Hrabia

2021

IJMS

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“…Collagens, laminins, fibronectin, and matrix metalloproteinases (MMPs) are the main components of ECM and take part in regulating granulosa cell (GC) behavior and function including migration, differentiation, survival, and steroidogenesis [8-12]. MMPs in particular play a crucial role in the breakdown of ECM substrates [10, 13, 14] and the regulation of cell migration in various tissue types [13, 15]. In the chicken, it has been suggested that MMP2 and MMP9 participate in the ECM remodeling that is required for follicular development [16].…”

Section: Introductionmentioning

confidence: 99%

miR-199-3p Suppresses Cellular Migration and Viability and Promotes Progesterone Production in Goose Ovarian Follicles Before Selection by Targeting ITGB8 and Regulating Other ECM-related Genes

Zhang

Zhao

et al. 2021

Preprint

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The extracellular matrix (ECM) constitutes the follicular basal lamina and is also present between follicular cells. Remodeling of the ECM is believed to be a key event in follicular development, especially follicular selection, and plays important roles in cell migration, survival, and steroidogenesis. miR-199-3p is differentially expressed in the goose follicular granulosa layer during follicular selection and is reported to play a primary role in inhibiting cell migration and invasion. Nevertheless, the effect of miR-199-3p on ovarian follicles and its role in follicular cellular migration are not understood. In this study, we demonstrated by qRT-PCR that miR-199-3p was differentially expressed in the granulosa layer from goose ovarian follicles before and after follicular selection. Additionally, we found that miR-199-3p overexpression could significantly suppress cell viability and migration, as well as elevate both the concentration of progesterone and the expression of key progesterone production genes in cultured granulosa cells (GCs) from goose pre-hierarchical follicles. Furthermore, using dual-fluorescence reporter experiments on 293T cells, we confirmed that miR-199-3p downregulated the expression of the ECM gene ITGB8 by directly targeting its mRNA three prime untranslated region (3′ UTR). Finally, we found that miR-199-3p overexpression in the GCs of goose pre-hierarchical follicles inhibited the expression of two ECM-related genes ( MMP9 and MMP15 ) yet promoted the expression of another two ECM-related genes ( COL4A1 and LAMA1 ). Taken together, these findings suggest that miR-199-3p participates in granulosa cell migration, viability, and steroidogenesis in goose ovarian follicles before selection by targeting ITGB8 and modulating other ECM-related genes. These data highlight the key roles of miR-199-3p in follicular cell migration, viability, and steroidogenesis by regulating ECM-related genes and thus contribute to a better understanding of the mechanisms underlying follicle selection in birds.

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Matrix metalloproteinase contribution in management of cancer proliferation, metastasis and drug targeting

Abdel-Hamid

Abass

2021

Mol Biol Rep

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Effect of eCG treatment on gene expression of selected matrix metalloproteinases (MMP-2, MMP-7, MMP-9, MMP-10, and MMP-13) and the tissue inhibitors of metalloproteinases (TIMP-2 and TIMP-3) in the chicken ovary

Cited by 19 publications

References 34 publications

Matrix Metalloproteinases (MMPs) and Inhibitors of MMPs in the Avian Reproductive System: An Overview

Matrix Metalloproteinases (MMPs) and Inhibitors of MMPs in the Avian Reproductive System: An Overview

miR-199-3p Suppresses Cellular Migration and Viability and Promotes Progesterone Production in Goose Ovarian Follicles Before Selection by Targeting ITGB8 and Regulating Other ECM-related Genes

Matrix metalloproteinase contribution in management of cancer proliferation, metastasis and drug targeting

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