Effect of ectomycorrhizal fungi on survival and growth of micropropagated plants and seedlings of Castanea sativa mill.

Martins, Anabela; Barroso, José G.; Pais, M. S.

doi:10.1007/s005720050135

Cited by 40 publications

(34 citation statements)

References 10 publications

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“…The effect of mycorrhization on plant growth is well documented (Garbaye et al, 1988, Bougher et al, 1990, Grove and Le Tacon, 1993Le Tacon et al, 1997;Généré, 1995;Martins et al, 1996;Généré et al, 1997;Parladé et al, 1997;Dell and Malajczuk, 1997). Bougher et al (1990) made several trials in controlled inoculation of Eucalyptus diversicolor seedlings with different fungal species and different P supplementations.…”

Section: Effect Of Mycorrhiza Inoculation On Plant Growthmentioning

confidence: 99%

“…Mycorrhization of micropropagated plants before acclimation increases survival, enhancing the functionality of the root system and the mineral plant nutrition (Rancillac, 1982;Grellier et al, 1984;Heslin and Douglas, 1986;Poissonier, 1986;Tonkin et al, 1989;Martins, 1992;2004;Martins et al, 1996;Herrmann et al, 1998;Díez et al, 2000). Similarly, in vitro mycorrhization of micropropagated plants can be used to increase survival and growth during ex vitro weaning (Nowak, 1998).…”

Section: Mycorrhization Of Micropropageted Plantsmentioning

confidence: 99%

“…Mycorrhization trials have been made with different micropropagated plant species: pine (Rancillac, 1982;Normand et al, 1996), birch (Grellier et al, 1984), poplar (Heslin and Douglas, 1986), eucalyptus (Poissonier, 1986;Tonkin et al, 1989), oak (Herrmann et al, 1998), chestnut (Strullu et al, 1986;Martins, 1992;2004;Martins et al, 1996;Martins and Pais, 2005), cork oak (Díez et al, 2000). These trials were performed as an effort to make micropropagation a sustainable propagation method for plant species recalcitrant to conventional propagation, increasing in vitro plant performances.…”

Section: Mycorrhization Of Micropropageted Plantsmentioning

confidence: 99%

“…Castanea sativa micropropagated plants were studied along 90 days of plant-fungus association in vitro, after preliminary studies on plant-fungus compatibility with four fungi species (Martins et al, 1996). The studies included: (1) development of mycorrhizal morphological structures (mantle and Hartig net) along 90 days; and (2) mycorrhizal influence on growth rates (heights, stem diameter, length of major root, total plant length, fresh weights and dry weights).…”

Section: Mycorrhization Of Micropropageted Plantsmentioning

confidence: 99%

“…The studies included: (1) development of mycorrhizal morphological structures (mantle and Hartig net) along 90 days; and (2) mycorrhizal influence on growth rates (heights, stem diameter, length of major root, total plant length, fresh weights and dry weights). (Martins et al, 1996).The fungi tested differed in their capacity to form mycorrhizas with C. sativa plants in vitro. P. tinctorius showed the best capacity to colonize chestnut roots either from seedlings or from micropropagated plants (Martins et al, 1996(Martins et al, , 1997.…”

Section: Mycorrhization Of Micropropageted Plantsmentioning

confidence: 99%

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In vitro Mycorrhization of Micropropagated Plants: Studies on Castanea sativa Mill

Martins

2008

Mycorrhizae: Sustainable Agriculture and Forestry

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Abstract:In vitro mycorrhization can be made by several axenic and nonaxenic techniques but criticism exists about their artificiality and inability to reproduce under natural conditions. However, artificial mycorrhization under controlled conditions can provide important information about the physiology of symbiosis. Micropropagated Castanea sativa plants were inoculated with the mycorrhizal fungus Pisolithus tinctorius after in vitro rooting. The mycorrhizal process was monitored at regular intervals in order to evaluate the mantle and hartig net formation, and the growth rates of mycorrhizal and nonmycorrhizal plants. Plant roots show fungal hyphae adhesion at the surface after 24 hours of mycorrhizal induction. After 20 days a mantle can be observed and a hartig net is forming although the morphology of the epidermal cells remains unaltered. At 30 days of root-fungus contact the hartig net is well developed and the epidermal cells are already enlarged. After 50 days of mycorrhizal induction, growth was higher for mycorrhizal plants than for nonmycorrhizal ones. The length of the major roots was lower in mycorrhizal plants after 40 days. Fresh and dry weights were higher in mycorrhizal plants after 30 days. The growth rates of chestnut mycorrhizal plants are in agreement with the morphological development of the mycorrhizal structures observed at each mycorrhizal time. The assessment of symbiotic establishment takes into account the formation of a mantle and a hartig net that were already developed at 30 days, when differences between fresh and dry weights of mycorrhizal and nonmycorrhizal plants can be quantified. In vitro conditions, mycorrhization influences plant physiology after 20 days of root-fungus contact, namely in terms of growth rates. Fresh and dry weights, heights, stem diameter and growth rates increased while major root growth rate decreased in mycorrhizal plants.

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