Effect of ectopic expression of NtEXPA5 gene on cell size and growth of organs of transgenic tobacco plants

Kuluev, B. R.; Safiullina, M. G.; Knyazev, A. V.; Чемерис, А. В.

doi:10.1134/s1062360413010049

Cited by 31 publications

(23 citation statements)

References 16 publications

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“…NbEXPA1 shares high amino acid sequence identity with the expansins NtEXPA5 and OsEXPA4 from N. tabacum and rice, respectively ( Figure S3). Constitutive overexpression of NtEXPA5 stimulates cell elongation, leading to increased leaf and stem sizes of transgenic tobacco plants (Kuluev et al, 2013). In transgenic rice with inducible OsEXPA4 expression, seedling growth is positively correlated with OsEXPA4 protein levels (Choi et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

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NbEXPA1, an α‐expansin, is plasmodesmata‐specific and a novel host factor for potyviral infection

Park

Renaud

et al. 2017

The Plant Journal

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Plasmodesmata (PD), unique to the plant kingdom, are structurally complex microchannels that cross the cell wall to establish symplastic communication between neighbouring cells. Viral intercellular movement occurs through PD. To better understand the involvement of PD in viral infection, we conducted a quantitative proteomic study on the PD-enriched fraction from Nicotiana benthamiana leaves in response to infection by Turnip mosaic virus (TuMV). We report the identification of a total of 1070 PD protein candidates, of which 100 (≥2-fold increase) and 48 (≥2-fold reduction) are significantly differentially accumulated in the PD-enriched fraction, when compared with protein levels in the corresponding healthy control. Among the differentially accumulated PD protein candidates, we show that an α-expansin designated NbEXPA1, a cell wall loosening protein, is PD-specific. TuMV infection downregulates NbEXPA1 mRNA expression and protein accumulation. We further demonstrate that NbEXPA1 is recruited to the viral replication complex via the interaction with NIb, the only RNA-dependent RNA polymerase of TuMV. Silencing of NbEXPA1 inhibits plant growth and TuMV infection, whereas overexpression of NbEXPA1 promotes viral replication and intercellular movement. These data suggest that NbEXPA1 is a host factor for potyviral infection. This study not only generates a PD-proteome dataset that is useful in future studies to expound PD biology and PD-mediated virus-host interactions but also characterizes NbEXPA1 as the first PD-specific cell wall loosening protein and its essential role in potyviral infection.

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Section: Discussionmentioning

confidence: 99%

“…NbEXPA1 has 97.6% identity with NtEXPA5 ( Figure S4). A recent study suggested that NbEXPA1 functions as a cell wall loosening protein that plays an important role in cell expansion (Kuluev et al, 2013).…”

Section: Nbexpa1 Colocalizes With the Vrc In Tumv-infected Cellsmentioning

confidence: 99%

NbEXPA1, an α‐expansin, is plasmodesmata‐specific and a novel host factor for potyviral infection

Park

Renaud

et al. 2017

The Plant Journal

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“…To date, a large number of expansin gene families has been identified and characterized in both model and crop plants, including Arabidopsis, rice and maize, to mention but a few. However, no such kind of analysis has been done in tobacco, except expression studies for a few NtEXPs genes (Kuluev et al 2013(Kuluev et al , 2014. Therefore, it is of great importance to identify the expansin gene family in the tobacco genome and explore the characteristics and functions of each expansin gene.…”

Section: Discussionmentioning

confidence: 99%

“…They are considered to bind to glucan-coated cellulose in the cell wall causing a reversible disruption of hydrogen bonding between cellulose microfibrils and glucan matrix and thus loosening the cell wall in a pH-dependent manner Cosgrove 2015). Expansins are commonly found in plants and are well known to play important roles in diverse biological processes related to cell wall modification, including seed germination , root growth and development Yu et al 2011), stem growth and internode elongation (Cho and Kende 1997;Kuluev et al 2014), leaf initiation and expansion (Fleming et al 1997;Kuluev et al 2013), flowering and flower size (Kuluev et al 2012), pollen germination and fertilization (Cosgrove 2000a), fruit growth and/or ripening (Rose et al 1997;Brummell et al 1999;Civello et al 1999;Ishimaru et al 2007). They have also been associated with nutrient uptake and efficiency (Zhou et al 2014), abiotic and biotic stress tolerance Zhao et al 2011;Lü et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Although genome-wide studies on expansins have been reported in many plant species, a systematic analysis of this gene family in tobacco (Nicotiana tabacum) has not yet been published to date, except expression studies for a few tobacco expansin genes (Kuluev et al 2013(Kuluev et al , 2014. The completion of high-quality draft genomes of tobacco (Sierro et al 2014) provides us the opportunity to uncover the expansin gene family and its characteristics in this allotetraploid crop.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum)

2016

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Expansins are pH-dependent cell wall loosening proteins which form a large family in plants. They have been shown to be involved in various developmental processes and been implicated in enabling plants' ability to absorb nutrients from the soil as well as conferring biotic and abiotic stress resistances. It is therefore clear that they can be potential targets in genetic engineering for crop improvement. Tobacco (Nicotiana tabacum) is a major crop species as well as a model organism. Considering that only a few tobacco expansins have been studied, a genome-wide analysis of the tobacco expansin gene family is necessary. In this study, we identified 52 expansins in tobacco, which were classified into four subfamilies: 36 NtEXPAs, 6 NtEXPBs, 3 NtEXLAs and 7 NtEXLBs. Compared to other species, the NtEXLB subfamily size was relatively larger. Phylogenetic analysis showed that the 52 tobacco expansins were divided into 13 subgroups. Gene structure analysis revealed that genes within subfamilies/subgroups exhibited similar characteristics such as gene structure and protein motif arrangement. Whole-genome duplication and tandem duplication events may have played important roles in the expanding of tobacco expansins. Cis-Acting element analysis revealed that each expansin gene was regulated or several expansin genes were co-regulated by both internal and environmental factors. 35 of these genes were identified as being expressed according to a microarray analysis. In contrast to most NtEXPAs which had higher expression levels in young organs, NtEXLAs and NtEXLBs were preferentially expressed in mature or senescent tissues, suggesting that they might play different roles in different organs or at different developmental stages. As the first step towards genome-wide analysis of the tobacco expansin gene family, our work provides solid background information related to structure, evolution and expression as well as regulatory cis-acting elements of the tobacco expansins. This information will provide a strong foundation for cloning and functional exploration of expansin genes in tobacco.

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Expansins: roles in plant growth and potential applications in crop improvement

2016

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Key messageResults from various expansin related studies have demonstrated that expansins present an opportunity to improve various crops in many different aspects ranging from yield and fruit ripening to improved stress tolerance.AbstractThe recent advances in expansin studies were reviewed. Besides producing the strength that is needed by the plants, cell walls define cell shape, cell size and cell function. Expansins are cell wall proteins which consist of four sub families; α-expansin, β-expansin, expansin-like A and expansin-like B. These proteins mediate cell wall loosening and they are present in all plants and in some microbial organisms and other organisms like snails. Decades after their initial discovery in cucumber, it is now clear that these small proteins have diverse biological roles in plants. Through their ability to enable the local sliding of wall polymers by reducing adhesion between adjacent wall polysaccharides and the part they play in cell wall remodeling after cytokinesis, it is now clear that expansins are required in almost all plant physiological development aspects from germination to fruiting. This is shown by the various reports from different studies using various molecular biology approaches such as gene achieve these many roles through their non-enzymatic wall loosening ability. This paper reviews and summarizes some of the reported functions of expansins and outlines the potential uses of expansins in crop improvement programs.

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Effect of ectopic expression of NtEXPA5 gene on cell size and growth of organs of transgenic tobacco plants

Cited by 31 publications

References 16 publications

NbEXPA1, an α‐expansin, is plasmodesmata‐specific and a novel host factor for potyviral infection

NbEXPA1, an α‐expansin, is plasmodesmata‐specific and a novel host factor for potyviral infection

Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum)

Expansins: roles in plant growth and potential applications in crop improvement

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