EFFECT OF EDTA ON THE GERMINATION OF AND OUTGROWTH FROM SPORES OF Clostridium botulinum 62‐A

Winarno, F.G.; Stumbo, C. R.; Hayes, Kirby M.

doi:10.1111/j.1365-2621.1971.tb03305.x

Cited by 14 publications

(3 citation statements)

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“…The presence of EDTA in the recovery medium increased the percentage of inhibition at pH 7.0 and 6.7 (Tab]e 1) in spores both with heat treatment (125°C, 30 s) and without, although the increase was significant (P :::; 0.05) only in untreated spores. As the table shows, there is a pH-EDTA interaction, as was also found by Winaro et al (21) with spores of Clostridium botulinum. However, the mechanisms of the pH-EDTA interaction still remain obscure.…”

Section: Effect Of Ph and Type Of Acidu/ant On Spore Recoverysupporting

confidence: 80%

Growth and Heat Resistance of Clostridium SporogenesPA 3679 Spores Heated and Recovered in Acidified Media

Sánchez

Rodrigo

Ocio

et al. 1995

Journal of Food Protection

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The effect of subculture medium composition (pH, type of acidulant, presence of EDTA) on the germination of spores of Clostridium sporogenes PA 3679, both with and without heat treatment in phosphate buffer (30 s at l25°C), was studied. Additionally, the effect of the heating medium substrate and pH and subculture medium pH and composition on the heat resistance of the spores was evaluated. The results indicate that the pH of the recovery medium and the type of acidulant have significant effects (P ≤ 0.05) on counts of PA 3679. Adding EDTA only increases inhibition of this microorganism when the pH is near neutral. For a given pH level, citric acid provided greater inhibiting power than glucono-δ-lactone, although the percentage of undissociated acid was less for citric acid. No growth was observed at pH 5.7, although in the case of glucono-δ-lactone sporadic colony development was seen in some plates. The pH of the heating medium acidified with glucono-δ-lactone did not affect the heat-resistance parameters (D and z) of spores heated to temperatures between 121 and 140°C in relation to the unacidified extract. Nor did we see any significant influence of the composition and pH of the recovery medium on these heat-resistance parameters when comparing the results with those obtained using the usual recovery medium.

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Section: Effect Of Ph and Type Of Acidu/ant On Spore Recoverysupporting

confidence: 80%

Growth and Heat Resistance of Clostridium SporogenesPA 3679 Spores Heated and Recovered in Acidified Media

Sánchez

Rodrigo

Ocio

et al. 1995

Journal of Food Protection

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“…In response to different germinants, spores of proteolytic Cl. botulinum germinated optimally at pH 6á5±7á5, but not below pH 4á8 or above pH 9á2 (Rowley and Feeherry 1970;Winarno et al 1971;Ando 1973a); strain differences were also identi®ed (Blocher and Busta 1985). A predictive model of the effect of temperature (15±30°C), pH (5á5±6á5) and NaCl (0á5±4á0%) on germination of spores of strain 56A in Brain Heart Infusion Broth has been published (Chea et al 2000).…”

Section: Discussionmentioning

confidence: 99%

“…In response to different germinants, spores of proteolytic Cl. botulinum germinated optimally at pH 6·5–7·5, but not below pH 4·8 or above pH 9·2 (Rowley and Feeherry 1970; Winarno et al . 1971; Ando 1973a); strain differences were also identified (Blocher and Busta 1985).…”

Section: Discussionmentioning

confidence: 99%

Use of a novel method to characterize the response of spores of non-proteolytic Clostridium botulinum types B, E and F to a wide range of germinants and conditions

Plowman

Peck

2002

J Appl Microbiol

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Aims: Limited information is available on the germination triggers for spores of nonproteolytic Clostridium botulinum. An automated system was used to study the effect of a large number of potential germinants, of temperature and pH, and aerobic and anaerobic conditions, on germination of spores of non-proteolytic Cl. botulinum types B, E and F. Methods and Results: A Bioscreen analyser was used to measure germination by decrease in optical density. Results were con®rmed by phase-contrast light microscopy. Spores of strains producing type B, E and F toxin gave similar results. Optimum germination occurred in. A further 12 combinations of factors induced germination. Sodium bicarbonate, sodium thioglycollate and heat shock each enhanced germination, but were not essential. Germination was similar in aerobic and anaerobic conditions. The optimum pH range was 5á5±8á0, germination occurred at 1±40°C, but not at 50°C, and was optimal at 20±25°C. Conclusions: The automated system enabled a systematic study of germination requirements, and provided an insight into germination in spores of non-proteolytic Cl. botulinum. Signi®cance and Impact of the Study: The results extend understanding of germination of non-proteolytic Cl. botulinum spores, and provide a basis for improving detection of viable spores.

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Possible Substitutes for Nitrite

Pegg

Shahidi

2004

Nitrite Curing of Meat

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EFFECT OF EDTA ON THE GERMINATION OF AND OUTGROWTH FROM SPORES OF Clostridium botulinum 62‐A

Cited by 14 publications

References 0 publications

Growth and Heat Resistance of Clostridium SporogenesPA 3679 Spores Heated and Recovered in Acidified Media

Growth and Heat Resistance of Clostridium SporogenesPA 3679 Spores Heated and Recovered in Acidified Media

Use of a novel method to characterize the response of spores of non-proteolytic Clostridium botulinum types B, E and F to a wide range of germinants and conditions

Possible Substitutes for Nitrite

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