Effect of eicosapentaenoic acid (EPA) on tight junction permeability in intestinal monolayer cells

Usami, Makoto; Muraki, Kayoko; Iwamoto, Mariko; Ohata, Atsushi; Matsushita, E.; Miki, Akinori

doi:10.1054/clnu.2001.0430

Cited by 66 publications

(52 citation statements)

References 23 publications

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“…This is the first report on the effect of soy, and DHA-and EPA-enriched PC on T Js. However, a few studies have examined on the effects of LCFA on TJs [20][21][22][23]. In the present study, DHA-and EPA-enriched LDH release in all three groups was both dose-and time-dependent.…”

Section: Discussionmentioning

confidence: 45%

Docosahexaenoic acid and eicosapentaenoic acid-enriched phosphatidylcholine liposomes enhance the permeability, transportation and uptake of phospholipids in Caco-2 cells

et al. 2006

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The influence of docosahexaenoic acid (DHA)-and eicosapentaenoic acid (EPA)-enriched phosphatidylcholine (PC) on the permeability, transport and uptake of phospholipids was evaluated in Caco-2 cells . The cells were grown on permeable polycarbonate transwell filters, thus allowing separate access to the apical and basolateral chambers . The monolayers of the cells were used to measure lucifer yellow permeability and transepithelial electrical resistance Our results show that DHA-and EPA-enriched PC increases tight junction permeability across the Caco-2 cell monolayer whereas soy PC has no effect on tight junction permeability .Transportation and uptake of DHA-and EPA-enriched PC SUV differed significantly (P<0.01) from those of soy PC SUV at all doses. We found that PC SUV transported across Caco-2 monolayer and was taken up by Caco-2 cells with very slight injury of the cell membrane up to 100 /-1M PC. Lactate dehydrogenase release and cell viability did not differ significantly between the treatment and control, emphasizing that injury was minimal. Our results suggest that DHA-and EPA-enriched PC enhance the permeability, transport and uptake of PC SUV across monolayers of Caco-2 cells.

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Section: Discussionmentioning

confidence: 45%

Docosahexaenoic acid and eicosapentaenoic acid-enriched phosphatidylcholine liposomes enhance the permeability, transportation and uptake of phospholipids in Caco-2 cells

et al. 2006

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“…Mean values with different letters are significantly different (P Ͻ 0.05). protein interactions (9), changes in eicosanoid production, protein kinase C activity (8,25), or increased oxidative stress (10,(47)(48)(49). However, the correlation between fatty acid enrichment, lipid peroxidation, and epithelial barrier function has not been considered to date.…”

Section: Discussionmentioning

confidence: 99%

“…Control and taurine-maintained cells were exposed to the same final BHT and ethanol concentration as DHA-supplemented medium. The range of DHA concentrations tested (up to 100 M) are those usually tested in similar fatty acid enrichment experiments in Caco-2 cells (8,(24)(25)(26) and are lower than that considered a physiological concentration (800 M) in similar experimental conditions (27).…”

Section: Dha and Taurine Enrichmentmentioning

confidence: 99%

“…Occludin and ZO-1 are two integral structural components of tight junctions with a crucial role in maintaining these epithelial functions (7). PUFAs have been shown to influence tight junction permeability by modifying either cyclooxygenase and lipooxygenase products (8) or occludin localization (9). Nevertheless, Rao et al (10) claimed that oxidative stress is the main factor responsible for the loss of epithelial barrier function through tyrosine phosphorylation and redistribution of tight junction proteins.…”

mentioning

confidence: 99%

“…Moreover, the participation of ROS and RNS in paracellular permeability regulation was also determined. Cultures of differentiated Caco-2 cells form a highly polarized epithelium with many of the properties of the intestinal villous absorptive cells (20) and constitute an in vitro experimental model, currently used to evaluate intestinal epithelial paracellular permeability (8,(21)(22)(23).…”

mentioning

confidence: 99%

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Lipid peroxidation induced by DHA enrichment modifies paracellular permeability in Caco-2 cells

Roig-Pérez

Guardiola

Moretó

et al. 2004

Journal of Lipid Research

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Dietary enrichment with docosahexaenoic acid (DHA) has numerous beneficial effects on health. However, the intake of high doses of polyunsaturated fatty acids can promote lipid peroxidation and the subsequent propagation of oxygen radicals. The purpose of this study was to evaluate the effect of DHA on lipid peroxidation and tight junction structure and permeability in Caco-2 cell cultures. Moreover, the effects of taurine, a functional ingredient with antioxidant properties, were also tested. Differentiated Caco-2 cell monolayers were maintained in DHA-supplemented conditions with or without added taurine. Incubation with 100 M DHA increased lipid peroxidation and paracellular permeability, in parallel with a redistribution of the tight junction proteins occludin and ZO-1. Taurine partially prevented all of these effects. The participation of reactive oxygen and nitrogen species in increased paracellular permeability was also examined using various agents that modify the formation of superoxide radical, hydrogen peroxide, nitric oxide, and peroxynitrite. We conclude that hydrogen peroxide and peroxynitrite may be involved in the DHA-induced increase in paracellular permeability and that the protective role of taurine may be in part related to its capacity to counteract the effects of hydrogen peroxide. -Roig-Pérez, S., F. Guardiola, M. Moretó, and R. Ferrer. Lipid peroxidation induced by DHA enrichment modifies paracellular permeability in Caco-2 cells: protective role of taurine.

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Testicular development evaluation in rats exposed to 60 Hz and 1 mT electromagnetic field

Tenório

Jimenez

Morais

et al. 2010

J of Applied Toxicology

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Society has been increasingly exposed to low-frequency electromagnetic fields (EMF), mainly from electricity distribution networks and electro-electronic devices. Aiming to clarify the extension of possible interactions between EMF and testicular development, this study evaluated the effects of exposure to 60 Hz and 1 mT EMF in the maturation of testicular components. Wistar rats were exposed to EMF three times per day for 30 min, between the 13th day of gestation and the 21st postnatal day. Results showed a decrease in the following parameters: tubular diameter and seminiferous tubules area; seminiferous epithelium height; total volume of seminiferous tubule; tubular lumen; seminiferous epithelium; and Leydig cells. On the other hand, an increase was observed in connective tissue cells and blood vessels volume. Plasma testosterone, Sertoli cells population, tubular length and gonadosomatic index did not change when exposed to EMF. Histomorphometric analysis showed that exposure to EMF can promote a delay in testicular development.

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Effect of eicosapentaenoic acid (EPA) on tight junction permeability in intestinal monolayer cells

Cited by 66 publications

References 23 publications

Docosahexaenoic acid and eicosapentaenoic acid-enriched phosphatidylcholine liposomes enhance the permeability, transportation and uptake of phospholipids in Caco-2 cells

Docosahexaenoic acid and eicosapentaenoic acid-enriched phosphatidylcholine liposomes enhance the permeability, transportation and uptake of phospholipids in Caco-2 cells

Lipid peroxidation induced by DHA enrichment modifies paracellular permeability in Caco-2 cells

Testicular development evaluation in rats exposed to 60 Hz and 1 mT electromagnetic field

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