Effect of electric field induced transmembrane potential on spheroidal cells: theory and experiment

Valič, Blaž; Golzio, Muriel; Pavlin, Mojca; Schatz, A.; Faurie, Cécile; Gabriel, B.; Teissié, Justin; Rols, Marie‐Pierre; Miklavčič, Damijan

doi:10.1007/s00249-003-0296-9

Cited by 202 publications

(145 citation statements)

References 23 publications

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“…In addition, in vitro and in vivo studies have elucidated the effect of the shape, size and orientation of cells in tissue and cell density on electric field distribution, which are all crucial factors for effective electrotransfection. 24,26,30 We pointed out in a recent study 26 that the histological properties of tumors are responsible for different transfection efficiencies obtained and this may also be true for the different transfection efficiencies obtained in different tissues using the same electric pulse parameters.…”

Section: Electrotransfer Of Tumors M Cemazar Et Almentioning

confidence: 99%

“…Field effects are dependent on cell shape and size, the orientation of the cells versus the field orientation and the composition of the tissue. [24][25][26] Nevertheless, it has been suggested that the function of 'HV' and 'LV' pulses is the same for both tissues, the 'HV' pulse permeabilizes target cells and the 'LV' pulse electrophoretically transports the DNA to the cells.…”

Section: Introductionmentioning

confidence: 99%

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Control by pulse parameters of DNA electrotransfer into solid tumors in mice

et al. 2009

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Electrotransfer (electroporation) is recognized as one of the most promising alternatives to viral vectors for transfection of different tissues in vivo for therapeutic purposes. We evaluated the transfection efficiency of reporter genes (green fluorescent protein and luciferase) in murine subcutaneous tumors using different combinations of high-field (HV) (600-1400 V cm À1 , 100 ms, 8 pulses) and low-field (LV) (80-160 V cm À1 , 50-400 ms, 1-8 pulses) pulses and compared it to protocol using eight identical pulses of 600 V cm À1 and 5 ms duration (electro-gene therapy, EGT). Expression of GFP was determined using a fluorescent microscope and flow cytometry and expression of luciferase by measuring its activity using a luminometer. The EGT protocol yielded the highest expression of both reporter genes. However, a careful optimization of combinations of HV and LV pulses may result in similar transfection as EGT pulses. With the combination protocol, relatively high fields of LV pulses were necessary to obtain comparable transfection to the EGT protocol. Expression of reporter genes was higher in B16 melanoma than in SA-1 fibrosarcoma. Our data support the hypothesis that both electropermeabilization and electrophoresis are involved in electrotransfer of plasmid DNA, but demonstrate that these components have to happen at the same time to obtain significant expression of the target gene in tumors.

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Section: Electrotransfer Of Tumors M Cemazar Et Almentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Control by pulse parameters of DNA electrotransfer into solid tumors in mice

et al. 2009

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“…30 On the other hand, in vitro studies have exposed the effect of the shape, size and orientation of cells in tissue and cell density. 31,32 Therefore, an important challenge for efficient gene therapy in solid tumors is to identify the structural and physiological properties of the tumor tissue. Better understanding of tissue barriers might be useful for the prediction of transgene expression patterns in certain tumor types in vivo, as well as for further optimization of gene delivery systems.…”

Section: Introductionmentioning

confidence: 99%

The effect of the histological properties of tumors on transfection efficiency of electrically assisted gene delivery to solid tumors in mice

et al. 2007

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Uniform DNA distribution in tumors is a prerequisite step for high transfection efficiency in solid tumors. To improve the transfection efficiency of electrically assisted gene delivery to solid tumors in vivo, we explored how tumor histological properties affected transfection efficiency. In four different tumor types (B16F1, EAT, SA-1 and LPB), proteoglycan and collagen content was morphometrically analyzed, and cell size and cell density were determined in paraffin-embedded tumor sections under a transmission microscope. To demonstrate the influence of the histological properties of solid tumors on electrically assisted gene delivery, the correlation between histological properties and transfection efficiency with regard to the time interval between DNA injection and electroporation was determined. Our data demonstrate that soft tumors with larger spherical cells, low proteoglycan and collagen content, and low cell density are more effectively transfected (B16F1 and EAT) than rigid tumors with high proteoglycan and collagen content, small spindle-shaped cells and high cell density (LPB and SA-1). Furthermore, an optimal time interval for increased transfection exists only in soft tumors, this being in the range of 5-15 min. Therefore, knowledge about the histology of tumors is important in planning electrogene therapy with respect to the time interval between DNA injection and electroporation.

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“…Later studies added more complexity to the modeled cell and provided insight into the factors affecting the induced transmembrane potential. This included both the electrical [5,[9][10][11][12] and geometrical properties of the cell, such as its shape [13,14] and orientation to the field [15,16]. These works stressed the importance of cellular properties in the build-up of the transmembrane potential.…”

Section: Introductionmentioning

confidence: 99%

Influence of Cellular Properties on the Electric Field Distribution Around a Single Cell

Ye¹,

Cotic²,

Fehlings³

et al. 2012

PIER B

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Abstract-Electric fields have been widely used for the treatment of neurological diseases, using techniques such as non-invasive brain stimulation. An electric current controls cell excitability by imposing voltage changes across the cell membrane. At the same time, the presence of the cell itself causes a re-distribution of the local electric field. Computation of the electric field distribution at a single cell microscopic level is essential in understanding the mechanism of electric stimulation. In addition, the impact of the cellular biophysical properties on the field distribution in the vicinity of the cell should also be addressed. In this paper, we have begun by first computing the field distribution around and within a spherical model cell. The electric fields in the three regions differed by several orders of magnitude. The field intensity in the extracellular space was of the same order as that of the externally applied field, while in the membrane, it was calculated to be several thousand times greater than the applied field. In contrast, the field intensity inside the cell was greatly attenuated to approximately 1/133th of the applied field. We then performed a

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Effect of electric field induced transmembrane potential on spheroidal cells: theory and experiment

Cited by 202 publications

References 23 publications

Control by pulse parameters of DNA electrotransfer into solid tumors in mice

Control by pulse parameters of DNA electrotransfer into solid tumors in mice

The effect of the histological properties of tumors on transfection efficiency of electrically assisted gene delivery to solid tumors in mice

Influence of Cellular Properties on the Electric Field Distribution Around a Single Cell

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