Effect of electric stimulus on human adipose‐derived mesenchymal stem cells cultured in 3D‐printed scaffolds

Abstract

Electrical stimulation has shown great potential for nerve regeneration processes. This makes it attractive to use electrically active materials in the neural scaffold. In this paper, bismuth ferrite (BFO) nanoparticles were synthesized via co‐precipitation method and incorporated to 10 wt% polylactic acid (PLA) in chloroform to obtain 3D‐printed PLA/BFO biocomposites. The crystallinity of BFO nanoparticles was confirmed by XRD, and we studied its chemical structure with FTIR, as well as the mechanical properties of the 3D‐printed composites. in vitro studies show that 3D‐printed scaffolds have no cytotoxicity and support the proliferation of human adipose‐derived mesenchymal stem cells (hADMSCs). Furthermore, 3D scaffolds embedded with BFO shows the highest cell viability relative to pristine PLA and BFO‐lined PLA scaffolds. A 48 hours electrical stimulation on the hADMSC cultured inside the 3D‐printed BFO‐lined PLA scaffolds indicates that stimulated cells are aligned toward the BFO line. These results could indicate the potential of BFO for directing cells toward damaged tissues.

In vitro electrically controlled amoxicillin release from 3D-printed chitosan/bismuth ferrite scaffolds

“…To investigate the interaction of chemical and molecular structure in produced CHT scaffolds, FT-IR analysis was carried out on the four groups. These scaffolds that were containing different concentrations of BFO, present the following specific vibrations: the characteristic absorption bands at 1565 𝑐𝑚 −1 , 1375 𝑐𝑚 -1 852 𝑐𝑚 -1 , which are associated with familiar vibrations for C≡CH, N-H and saccharide [14]. The distinctive peak is in the absorption band at 815 cm −1 in the spectrum for all experimental groups.…”

Controlled Delivery of Amoxicillin and Rifampicin by Three‐Dimensional Polyvinyl alcohol/Bismuth Ferrite Scaffolds

“…In addition, it was observed that 13 %% PVA/ (0.075)% BFO_RIF scaffolds (45.54 %) had lower strain at break compared to 13 % PVA/(0.075)% BFO_AMX scaffolds (53.24 %). The low interfacial adhesion of PVA and BFO particles is due to poor bonding between PVA and BFO particles [13] . As a result, the addition of BFO to the scaffolds decreased strain at break values since the interaction of PVA and BFO was less.…”

“…The low interfacial adhesion of PVA and BFO particles is due to poor bonding between PVA and BFO particles. [13] As a result, the addition of BFO to the scaffolds decreased strain at break values since the interaction of PVA and BFO was less.…”

“…[12] Recent research has shown that materials with both magnetic and electrical properties are of interest to researchers looking to develop new materials or structures due to their controlled abilities. [13] Multiferroics are materials with electrical, magnetic, and structural order properties that simultaneously produce ferroelectric, ferromagnetism, and ferroelasticity effects in the same material. [14] Bismuth ferrite (BFO) is a multiferroic material at room temperature with magnetically arranged and high thermal stability ferroelectric states.…”

“…Due to these benefits, nanoparticles have emerged as potential new medication carriers in therapeutic settings [12] . Recent research has shown that materials with both magnetic and electrical properties are of interest to researchers looking to develop new materials or structures due to their controlled abilities [13] . Multiferroics are materials with electrical, magnetic, and structural order properties that simultaneously produce ferroelectric, ferromagnetism, and ferroelasticity effects in the same material [14] .…”