Effect of Electroacupuncture on Bladder Dysfunction via Regulation of MLC and MLCK Phosphorylation in a Rat Model of Type 2 Diabetes Mellitus

Han, Xuke; Gao, Yang; Yin, Xuan; Wang, Sheng-Ju; Zhang, Xiaoran; Qiu, Chen

doi:10.1155/2021/5558890

Cited by 5 publications

(7 citation statements)

References 58 publications

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“…The results showed that the T2DM rat model was successfully induced in this experiment, indicating that the current disease status in rats is like the pathophysiological state of human T2DM combined with DBD, which is consistent with our previous findings ( Han et al, 2021 ).…”

Section: Resultssupporting

confidence: 92%

“…Consequently, these data suggest that unbalanced PACAP38 levels coupled with bladder remodeling may be a pathophysiological mechanism in DBD. Our previous study confirmed that EA could effectively alleviate bladder dysfunction in diabetic rats ( Han et al, 2021 ). Moreover, another study found that EA can regulate the urodynamics of neurogenic bladder model rats by up-regulating the protein expressions of PACAP38 and PAC1-R ( Liu et al, 2017 ).…”

Section: Discussionsupporting

confidence: 67%

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Effects of electroacupuncture on bladder dysfunction and the expression of PACAP38 in a diabetic rat model

Han

Chen

et al. 2023

Front. Physiol.

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Objective: To explore the effects and the possible mechanism of electroacupuncture (EA) on diabetic bladder dysfunction (DBD) in streptozotocin-high fat diet (STZ-HFD) induced type 2 diabetes mellitus (T2DM) rats.Methods: The experiment was divided into Control, diabetic bladder dysfunction, electroacupuncture, and Sham electroacupuncture group. After 8 weeks of electroacupuncture intervention, the body mass, 24 h urine volume, intraperitoneal glucose tolerance test (IPGTT), and urodynamics were detected. After the wet weight of the bladder was detected, the hematoxylin-eosin (HE), Masson’s trichrome, and TUNEL were used to analyze histological changes. The PACAP38 expressions in the bladder were detected by Real-time PCR and Western blot.Results: Compared to the Control group, the bladder wet weight, 24 h urine volume, blood glucose, maximum bladder capacity, bladder compliance, bladder wall thickness, the smooth muscle/collagen ratio, and apoptosis rate of the diabetic bladder dysfunction group were significantly increased. Moreover, the body mass and leak point pressure were significantly reduced. Compared with the Sham electroacupuncture group, the bladder wet weight, maximum bladder capacity, bladder compliance, bladder wall thickness, and apoptosis rate of the electroacupuncture group were significantly reduced. In contrast, the leak point pressure was increased. The PACAP38 mRNA and PACAP38 protein expression of the diabetic bladder dysfunction group were significantly lower than the Control group, while electroacupuncture treatment could upregulate PACAP38 mRNA levels and PACAP38 protein expression of diabetic bladder dysfunction model rats.Conclusion: electroacupuncture could ameliorate bladder dysfunction in the diabetic bladder dysfunction model rats by reversing bladder remodeling, which might be mainly mediated by regulating the PACAP38 level.

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Section: Resultssupporting

confidence: 92%

Section: Discussionsupporting

confidence: 67%

Effects of electroacupuncture on bladder dysfunction and the expression of PACAP38 in a diabetic rat model

Han

Chen

et al. 2023

Front. Physiol.

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“…As previously stated, neuropathy is one of the underlying causes of diabetes related bladder dysfunction. Neuromodulation was shown to restore voiding function in several studies (Chen et al 2013 ; Hsieh et al 2016 ; Han et al 2021 ). Voiding efficacy in control animals was found about 75% while it was measured as 30.02 ± 7.89% after 8 weeks of diabetes and 19.98 ± 6.03% after 18 weeks of diabetes in STZ-induced T1DM male rats (Chen et al 2013 ).…”

Section: Emerging Pathophysiology-based Treatments Of Lutd In Diabetesmentioning

confidence: 99%

“…All of the abnormalities were improved by electroacupuncture therapy and its beneficial effect was ascribed to regulation of myosin light chain and myosin light chain kinase phosphorylation. Moreover, electroacupuncture was reduced infiltration of inflammatory cells in bladder (Han et al 2021 ). These studies suggests that electrical stimulation of bladder may prevent or improve bladder dysfunction in diabetic individuals by facilitating bladder contraction.…”

Section: Emerging Pathophysiology-based Treatments Of Lutd In Diabetesmentioning

confidence: 99%

Established and emerging treatments for diabetes-associated lower urinary tract dysfunction

Erdogan

Liu

Arioglu‐Inan

et al. 2022

Naunyn-Schmiedeberg's Arch Pharmacol

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Dysfunction of the lower urinary tract (LUT) including urinary bladder and urethra (and prostate in men) is one of the most frequent complications of diabetes and can manifest as overactive bladder, underactive bladder, urinary incontinence, and as aggravated symptoms of benign prostate hyperplasia. We have performed a selective literature search to review existing evidence on efficacy of classic medications for the treatment of LUT dysfunction in diabetic patients and animals, i.e., α1-adrenoceptor and muscarinic receptor antagonists, β3-adrenoceptor agonists, and phosphodiesterase type 5 inhibitors. Generally, these agents appear to have comparable efficacy in patients and/or animals with and without diabetes. We also review effects of antidiabetic medications on LUT function. Such studies have largely been performed in animal models. In the streptozotocin-induced models of type 1 diabetes, insulin can prevent and reverse alterations of morphology, function, and gene expression patterns in bladder and prostate. Typical medications for the treatment of type 2 diabetes have been studied less often, and the reported findings are not yet sufficient to derive robust conclusions. Thereafter, we review animal studies with emerging medications perhaps targeting diabetes-associated LUT dysfunction. Data with myoinositol, daidzein, and with compounds that target oxidative stress, inflammation, Rac1, nerve growth factor, angiotensin II receptor, serotonin receptor, adenosine receptor, and soluble guanylyl cyclase are not conclusive yet, but some hold promise as potential treatments. Finally, we review nonpharmacological interventions in diabetic bladder dysfunction. These approaches are relatively new and give promising results in preclinical studies. In conclusion, the insulin data in rodent models of type 1 diabetes suggest that diabetes-associated LUT function can be mostly or partially reversed. However, we propose that considerable additional experimental and clinical studies are needed to target diabetes itself or pathophysiological changes induced by chronic hyperglycemia for the treatment of diabetic uropathy.

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“…Bladder mucosa is composed of transitional epithelium (urothelium), basement membrane, and lamina propria (LP) [8]. Structural analysis has revealed that the LP contains a rich neurovascular network, elastic fibers, and smooth muscle bundles (muscularis mucosa) [8][9][10], and recent studies suggest that increased cellular degeneration and necrosis of bladder mucosal cells may underlie bladder microvascular and nerves damage in diabetes [11]. In addition, microvascular injury may impair bladder innervation, alter detrusor function, and lead to urothelial dysfunction [12].…”

Section: Introductionmentioning

confidence: 99%

Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice

Kwon¹,

Choi

Liu

et al. 2022

Int Neurourol J

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Purpose: To assess functional and structural changes in vascular and neural structures associated with diabetic bladder dysfunction (DBD) in the bladders of streptozotocin (STZ)-induced diabetic mice.Methods: Eight-week-old C57BL/6 mice were injected with STZ at 50 mg/kg daily for 5 consecutive days. Catheters were inserted 12 weeks later, and 5 days after catheter placement bladder functions were assessed by conscious cystometry. Neurovascular and extracellular matrix marker changes in harvested urinary bladders were investigated by immunofluorescent staining. Body weights and fasting and postprandial blood glucose levels were measured 12 weeks after STZ injection.Results: STZ-induced diabetic mice had significantly lower body weights and significantly higher blood glucose levels. Assessment of bladder function in STZ-induced diabetic mice revealed a nearly 3-fold increase in bladder capacity and intercontractile interval compared to controls. However, basal pressure, maximal bladder pressure, and threshold pressure were not significantly different. Morphological and structural analysis showed that STZ-induced diabetic mice had significantly reduced microvascular density in lamina propria (33% of the nondiabetic control values), and severely decreased nerve contents in the detrusor region (42% of the nondiabetic control values).Conclusions: STZ-induced diabetic mice exhibit functional and structural derangements in urinary bladder. The present study provides a foundation and describes a useful means of evaluating the efficacies of therapeutic targets and exploring the detailed mechanism of DBD.

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Effect of Electroacupuncture on Bladder Dysfunction via Regulation of MLC and MLCK Phosphorylation in a Rat Model of Type 2 Diabetes Mellitus

Cited by 5 publications

References 58 publications

Effects of electroacupuncture on bladder dysfunction and the expression of PACAP38 in a diabetic rat model

Effects of electroacupuncture on bladder dysfunction and the expression of PACAP38 in a diabetic rat model

Established and emerging treatments for diabetes-associated lower urinary tract dysfunction

Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice

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