Effect of Electroacupuncture on the Expression of Agrin and Acetylcholine Receptor Subtypes in Rats with Tibialis Anterior Muscular Atrophy Induced by Sciatic Nerve Injection Injury

Wang, Meng; Liu, Junying; Zhang, Xiaoming; Yang, Shengbo

doi:10.1136/acupmed-2015-011005

Cited by 16 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Yu J. et al applied E-AT on penicillin injection-induced sciatic nerve injury model and the treatment alleviated muscular atrophy by upregulating agrin and acetylcholine receptor- ε (AChR- ε ), and downregulating AChR- γ associated in neuromuscular transmission [28]. In Cao R. et al, E-AT on sciatic nerve injury inhibited muscle cell apoptosis by suppressing Bax, cytochrome C, and caspase-3 [31].…”

Section: Resultsmentioning

confidence: 99%

A Review of Complementary and Alternative Medicine Therapies on Muscular Atrophy: A Literature Review of In Vivo/In Vitro Studies

Jeong

Seo

Park

et al. 2018

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Objective The objective of this review is to evaluate the recent treatment and study trends of complementary and alternative medicine (CAM) treatments on muscular atrophy by reviewing in vivo/in vitro studies. Materials and Methods The searches were conducted via electronic databases including PubMed, the Cochrane Library, China National Knowledge Infrastructure (CNKI), Wanfang MED, and five Korean databases. Only in vivo and in vitro studies were included in this study. Results A total of 44 studies (27 in vivo studies, 8 in vitro studies, and 9 in vivo with in vitro) were included. No serious maternal or fetal complications occurred. There were various animal models induced with muscular atrophy through “hindlimb suspension”, “nerve damage”, ‘alcohol or dexamethasone treatment', “diabetes”, “CKD”, “stroke”, “cancer”, “genetic modification”, etc. In 28 of 36 articles measuring muscle mass, CAM significantly increased the mass. Additionally, 10 of them showed significant improvement in muscle function. In most in vitro studies, significant increases in both the diameter of myotubes and muscle cell numbers were reported. The mechanisms of action of protein synthesis, degradation, autophagy, and apoptotic markers were also investigated. Conclusions These results demonstrate that CAM could prevent muscular atrophy. Further studies about CAM on muscular atrophy are needed.

show abstract

Section: Resultsmentioning

confidence: 99%

A Review of Complementary and Alternative Medicine Therapies on Muscular Atrophy: A Literature Review of In Vivo/In Vitro Studies

Jeong

Seo

Park

et al. 2018

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…The CSA of the tibialis anterior muscle was measured after hematoxylin and eosin (HE) staining of 4-m slices of paraffin-embedded specimens (prepared as described below). Forty fields were randomly chosen for measurement on each HE-stained section [26].…”

Section: Evaluation Of Muscle Atrophymentioning

confidence: 99%

Nano PGE1 Enhances Phosphorylation of ERK1/2 and Akt to Promote Recovery from Motor Dysfunction and Muscle Atrophy Induced by Sciatic Nerve Injury

Takenaga¹,

Ishihara²,

Niimi³

et al. 2019

Preprint

View full text Add to dashboard Cite

The effect of prostaglandin E1 (PGE1) encapsulated in nanoparticles (Nano PGE1) on motor dysfunction and muscle atrophy induced by sciatic nerve injury (SNI) was investigated in rats, and was compared with PGE1 encapsulated in lipid microspheres (Lipo PGE1) or PGE1 clathrated in cyclodextrin (PGE1-CD). The hind limb muscle weight ratio decreased until 2 weeks after SNI. All 3 PGE1 formulations significantly improved SNI-induced motor dysfunction. Nano PGE1 significantly promoted recovery from muscle atrophy at 2 and 3 weeks after SNI. Lipo PGE1 was also effective, but multiple doses were required. Compared with the SNI control group, the Nano PGE1 group showed upregulation of vascular endothelial growth factor (VEGF) and agrin expression in the injured sciatic nerve and atrophic muscles. Nano PGE1 accumulated prominently at the site of nerve injury and persisted for longer than Lipo PGE1 or PGE1-CD. Expression of all EP receptors was detected in the normal sciatic nerve, and EP2 expression increased after SNI. Finally, Nano PGE1 promoted ERK1/2 and Akt phosphorylation. These findings suggest that PGE1 released from nanoparticles accumulates at sites of nerve injury and increases VEGF production by augmenting ERK1/2 phosphorylation via EP receptor signaling, thus promoting tissue repair and regeneration.

show abstract

“…Numerous studies have demonstrated that acupuncture with low frequency electrical stimulation (Acu/LFES) can correct muscle atrophy in human and animals with various diseases, including diabetes and chronic kidney disease-induced muscle wasting ( Hu et al, 2015 ; Su et al, 2015 ), hindlimb suspension induced muscle loss ( Onda et al, 2011 ), facioscapulohumeral muscle dystrophy ( Liu et al, 2019 ), and amyotrophic lateral sclerosis or sciatic nerve injury caused muscle atrophy ( Su et al, 2016 ; Sudhakaran, 2017 ; Yu et al, 2017 ). Acu/LFES is used worldwide as a therapeutic intervention to reduce stress and other health problems ( NIH Consensus Conference, 1998 ).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of let-7 by Electrical Acupuncture Increases Protein Synthesis in Mice

Huang

Kuma

et al. 2021

Front. Physiol.

View full text Add to dashboard Cite

BackgroundOur previous study found that acupuncture with low frequency electrical stimulation (Acu/LFES) prevents muscle atrophy by attenuation of protein degradation in mice. The current study examines the impact of Acu/LFES on protein synthesis.MethodC57/BL6 mice received Acu/LFES treatment on hindlimb for 30 min once. Acu/LFES points were selected by WHO Standard Acupuncture Nomenclature and electric stimulation applied using an SDZ-II Electronic acupuncture instrument. Muscle protein synthesis was measured by the surface-sensing of translation (SUnSET) assay. Exosomes were isolated using serial centrifugation and concentration and size of the collected exosomes were measured using a NanoSight instrument. The mature microRNA library in serum exosomes was validated using a High Sensitivity DNA chip.ResultsProtein synthesis was enhanced in the both hindlimb and forelimb muscles. Blocking exosome secretion with GW4869 decreased the Acu/LFES-induced increases in protein synthesis. MicroRNA-deep sequencing demonstrated that four members of the Let-7 miRNA family were significantly decreased in serum exosomes. Real time qPCR further verified Acu/LFES-mediated decreases of let-7c-5p in serum exosomes and skeletal muscles. In cultured C2C12 myotubes, inhibition of let-7c not only increased protein synthesis, but also enhanced protein abundance of Igf1 and Igf1 receptors. Using a luciferase reporter assay, we demonstrated that let-7 directly inhibits Igf1.ConclusionAcu/LFES on hindlimb decreases let-7-5p leading to upregulation of the Igf1 signaling and increasing protein synthesis in both hindlimb and forelimb skeletal muscles. This provides a new understanding of how the electrical acupuncture treatment can positively influence muscle health.

show abstract

Effect of Electroacupuncture on the Expression of Agrin and Acetylcholine Receptor Subtypes in Rats with Tibialis Anterior Muscular Atrophy Induced by Sciatic Nerve Injection Injury

Cited by 16 publications

References 15 publications

A Review of Complementary and Alternative Medicine Therapies on Muscular Atrophy: A Literature Review of In Vivo/In Vitro Studies

A Review of Complementary and Alternative Medicine Therapies on Muscular Atrophy: A Literature Review of In Vivo/In Vitro Studies

Nano PGE1 Enhances Phosphorylation of ERK1/2 and Akt to Promote Recovery from Motor Dysfunction and Muscle Atrophy Induced by Sciatic Nerve Injury

Downregulation of let-7 by Electrical Acupuncture Increases Protein Synthesis in Mice

Contact Info

Product

Resources

About