Effect of electroporation on viability and bioconversion of isoflavones in mannitol‐soymilk fermented by lactobacilli and bifidobacteria

Abstract

Application of electroporation on lactobacilli and bifidobacteria could be beneficial for the development of fermented soymilk with enhanced bioactivity. Considering the enhanced bioactive aglycones, this soymilk could be useful for the prevention of hormone-dependent disorders.

The contribution of lipid peroxidation to membrane permeability in electropermeabilization: A molecular dynamics study

“…Experimental studies suggest that lipid peroxidation could present a mechanism of the long-lived cell membrane permeability, which persists for minutes after exposing the cells to electric pulses [20][21][22][23][24][25][26][27][28][29][30][31][32]. The aim of our study is to investigate whether peroxidized lesions in the cell membrane could be permeable enough to account for the post-pulse membrane permeability and conductance measured in electropermeabilized cells.…”

“…The complete resealing of the cell membrane takes several minutes at room temperature [15][16][17], which is about 8−9 orders of magnitude longer than the time of electropore closure as reported from MD investigations [18,19]. Experimental studies suggest that a possible alternative mechanism to explain the observed long-lived permeability of cell membranes is lipid peroxidation [20][21][22][23][24][25][26][27][28][29][30][31][32].…”

“…The fact that electroporation is accompanied by lipid peroxidation has been confirmed in bacteria [20,21], plant cells [22,23], and mammalian cells [23][24][25], as well as in liposomes made from polyunsaturated lipids [24][25][26][27]. Lipid peroxidation can be promoted by reactive oxygen species (ROS) already present in the solution before the delivery of electric pulses [26], although the exact mechanisms are not yet determined.…”

Electroporation-based applications in biotechnology

“…By contrast, the pores in the cell wall, particularly in the peptidoglycan wall of Gram-positive bacteria, are much more stable and can be visualized under an electron microscope [16] (Figure 2). …”

“…Scanning electron micrographs show Lactobacillus casei bacteria before (A) and after (B) exposure to an electric field pulse of 7.5 kV/cm amplitude and 4 ms duration. Reprinted, with permission from[16]. Scale bar, 2 mm.…”