Effect of Electrospun Fibrous Scaffolds with Different Fiber Orientations on the Alignment of Microvessel-Like Structures

Wu, Zhenzhen; Yang, Tao; Bao, Chongyun; Li, Mingzheng; Xu, Hockin H.K.; Liao, Xuejuan; Li, Long; Huang, Ranran; Pan, Jian; Liu, Xian

doi:10.1007/s40846-017-0284-7

Cited by 3 publications

(2 citation statements)

References 39 publications

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“…This may be attributable to the fact that the aligned fibers provide more adhesion sites for EC growth than randomly distributed fibers. 54,55 The scaffold was cut axially to obtain the images, as shown in Figure 5. After one day, larger numbers of cells were observed on FLTSs with no significant differences comparing to control groups, as depicted in Figure 5(g).…”

Section: Discussionmentioning

confidence: 99%

Fabrication of multilayer tubular scaffolds with aligned nanofibers to guide the growth of endothelial cells

Zeng

et al. 2020

J Biomater Appl

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Aligned electrospun fibers used for the fabrication of tubular scaffolds possess the ability to regulate cellular alignment and relevant functional expression, with applications in tissue engineering. Despite significant progress in the fabrication of small-diameter vascular grafts (SDVGs) over the past decade, several challenges remain; one of the most problematic of these is the fabrication of aligned nanofibers for multilayer SDVGs. Furthermore, delamination between each layer is difficult to avoid during the fabrication of multilayer structures. This study introduces a new fabrication method for minute delamination four-layer tubular scaffolds (FLTSs) that consist of an interior layer with highly longitudinal aligned nanofibers, two middle layers composed of electrospun sloped and circumferentially aligned fibers, and an exterior layer comprising random fibers. These FLTSs are used to simulate the structures and functions of native blood vessels. Here, thermoplastic polyurethane (TPU)/polycaprolactone (PCL)/polyethylene glycol (PEG) were electrospun to fabricate FLTSs or tubular scaffolds with completely random fibers layer (RLTSs). The surface wettability of the TPU/PCL/PEG tubular scaffold was tested by water contact angle analysis. In particular, compared with RLTSs, FLTSs showed excellent mechanical properties, with higher circumferential and longitudinal tensile properties. Furthermore, the high viability of the human umbilical vein endothelial cells (HUVECs) on the FLTSs indicated the biocompatibility of the tubular scaffolds comparing to RLTSs. The aligned and random composite structure of the FLTSs are conducive to promoting the growth of HUVECs, and the cell adhesion and proliferation on these scaffolds was found to be superior to that on RLTSs. These results demonstrate that the fabricated FLTSs have the potential for application in vascular tissue regeneration and clinical arterial replacements.

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Section: Discussionmentioning

confidence: 99%

Fabrication of multilayer tubular scaffolds with aligned nanofibers to guide the growth of endothelial cells

Zeng

et al. 2020

J Biomater Appl

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“…Micro vessels formed well on both meshes and the vessels on the former were also distributed along the fibers in an aligned manner. These properties provided multiple choices for the construction of pre-vascularized SDVGs ( Wu et al, 2017 ). The graft exhibited great cell viability and compatibility, suggesting a potentially available VG for application.…”

Section: Pluripotent Stem Cells In Es/sc Sdvgsmentioning

confidence: 99%

Small diameter vascular grafts: progress on electrospinning matrix/stem cell blending approach

Wang,

Chen,

et al. 2024

Front. Bioeng. Biotechnol.

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The exploration of the next-generation small diameter vascular grafts (SDVGs) will never stop until they possess high biocompatibility and patency comparable to autologous native blood vessels. Integrating biocompatible electrospinning (ES) matrices with highly bioactive stem cells (SCs) provides a rational and promising solution. ES is a simple, fast, flexible and universal technology to prepare extracellular matrix-like fibrous scaffolds in large scale, while SCs are valuable, multifunctional and favorable seed cells with special characteristics for the emerging field of cell therapy and regenerative medicine. Both ES matrices and SCs are advanced resources with medical application prospects, and the combination may share their advantages to drive the overcoming of the long-lasting hurdles in SDVG field. In this review, the advances on SDVGs based on ES matrices and SCs (including pluripotent SCs, multipotent SCs, and unipotent SCs) are sorted out, and current challenges and future prospects are discussed.

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Electrospinning Aligned SF/Magnetic Nanoparticles-Blend Nanofiber Scaffolds for Inducing Skeletal Myoblast Alignment and Differentiation

Yang,

Cheng,

Zhou

et al. 2024

ACS Appl. Bio Mater.

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In the realm of skeletal muscle tissue engineering, anisotropic materials that emulate natural tissues show substantial promise. Electrospun scaffolds, mimicking the fibrillar structure of the extracellular matrix, are commonly employed but often fall short in achieving optimal alignment and mechanical strength. Silk fibroin has emerged as a versatile material in tissue engineering, valued for its biocompatibility, mechanical robustness, and biodegradability. However, conventional electrospinning methods of SF result in randomly oriented fibers, limiting their efficacy. In this work, we developed a straightforward method to fabricate directional tissue scaffolds using silk fibroin. By integrating a magnetic field collecting device and incorporating Fe 3 O 4 nanoparticles into the spinning solution, we successfully produced wellaligned silk nanofiber scaffolds. These aligned fibers not only improved scaffold orientation and mechanical properties but also exhibited magnetic responsiveness. The aligned SF scaffolds effectively guided the adhesion, proliferation, and differentiation of mesenchymal stem cells along the fiber direction. Cultured on these scaffolds, myoblast C2C12 cells demonstrated oriented growth, highlighting the potential of aligned SF fibers in advancing skeletal muscle engineering for biomedical applications.

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Effect of Electrospun Fibrous Scaffolds with Different Fiber Orientations on the Alignment of Microvessel-Like Structures

Cited by 3 publications

References 39 publications

Fabrication of multilayer tubular scaffolds with aligned nanofibers to guide the growth of endothelial cells

Fabrication of multilayer tubular scaffolds with aligned nanofibers to guide the growth of endothelial cells

Small diameter vascular grafts: progress on electrospinning matrix/stem cell blending approach

Electrospinning Aligned SF/Magnetic Nanoparticles-Blend Nanofiber Scaffolds for Inducing Skeletal Myoblast Alignment and Differentiation

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