Effect of electrostatic atomization on ascorbate metabolism in postharvest broccoli

Abstract

In the present study, the effects of electrostatic atomization on ascorbate (AsA) metabolism were studied and the possible molecular mechanisms were discussed in broccoli (Brassica oleracea L. var. italica). With the treatment of electrostatic atomization, the yellowing process was delayed, and the ethylene production and respiration rate were significantly suppressed in broccoli after harvest. In the meanwhile, the AsA content declined rapidly to a lower level in the control after harvest, and the reduction of AsA was suppressed by the treatment with electrostatic atomization during the storage period. Additionally, the modulation of the AsA reduction by electrostatic atomization was highly regulated at the transcription level. The up-regulation of the AsA biosynthetic genes (BO-VTC1, BO-VTC2 and BO-GLDH), and AsA regeneration genes (BO-MDAR1, BO-MDAR2 and BO-DHAR) led to the suppression of AsA reduction in the electrostatic atomization treated broccoli after harvest. These results indicated that electrostatic atomization treatment might be a new effective approach for delaying the senescence of broccoli.

The time of the day to harvest affects the degreening, antioxidant compounds, and protein content during postharvest storage of broccoli

“…Variations in the level of ascorbic acid during the circadian rhythm has been observed (Chang, Yang, & Riskowski, ) and it has been hypothesized that these variations could be due to light–dark cycles and photosynthetic activity (Ivanov, ). In any case, the ascorbic content decreased in the same way in all samples during storage as senescence progressed, as it is described in other researches (Ma et al, , ).…”

Regulation of ascorbic acid metabolism by blue LED light irradiation in citrus juice sacs

“…AsA levels can be elevated in plants by increasing the gene expression of MDAR and DHAR [8] and [53]. In broccoli, the up-regulation of BO-MDAR1, BO-MDAR2, and BO-DHAR contributed to maintaining a high AsA content after harvest with an electrostatic atomization treatment [11]. In the present study, the results showed that the gene As shown in Fig.…”

“…As there is a mutation in the AsA biosynthetic enzyme L-galactono-1,4-lactone dehydrogenase (GLDH), humans cannot synthesize the AsA in the body and must obtain it through the daily diet. In recent years, to meet health requirements and the growing demand of consumers for highly nutritional foods, AsA metabolism in plants has been extensively investigated, and several efforts have been made to enhance AsA levels in fruits and vegetables [8], [9], [10], [11], [12] and [13].…”

“…1, AsA can be further oxidized by ascorbate peroxidase (APX) and ascorbate oxidase (AO), and regenerated by NADPH-dependent MDA reductase (MDAR) and dehydroascorbate reductase (DHAR) via the ascorbate-glutathione cycle [3], [4] and [18]. In broccoli and tomato, it has been found that the enzymes in the ascorbate-glutathione cycle exist as isoezymes distributed in different cellular organelles such as chloroplasts, plastids, mitochondria, and peroxisomes [11] and [19].…”

Effect of red and blue LED light irradiation on ascorbate content and expression of genes related to ascorbate metabolism in postharvest broccoli

“…(1-MCP) and electrostatic atomization were effective in delaying yellowing and decay, alleviating certain ethylene-induced postharvest physiological disorders, and suppressing the reduction of AsA in broccoli after harvest (Ma et al, 2009(Ma et al, , 2010(Ma et al, , 2012a.…”