Effect of elevated dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum on d-glucose and l-lactate

A disposable picolitre bioreactor for cultivation and investigation of industrially relevant bacteria on the single cell level

“…37 The high PLBR growth rate of m max = 0.63 ¡ 0.02 h 21 supports our assumption that bacteria remain in good physiological state in our microfluidic system. Actually, it also suggests that the microfluidic system offers better growth conditions than in typical lab-scale experiments.…”

Quantification of the transient and long-term response of Saccharomyces cerevisiae to carbon dioxide stresses of various intensities

“…Therefore, the observed respiration burst may reflect the energy requirement of the cells to face the CO 2 step-increase and not an uncontrolled storage sugars mobilization triggered by the CO 2 shift-up. CO 2 impact on microorganisms is widely attributed to its impact on the intracellular pH [17,19,[22][23][24] even though very few studies have measured this impact or challenged it on a quantitative basis [21].…”

“…Several mechanisms have been thought to contribute the CO 2 toxicity such as an "anesthesia" disturbing effect on the fluidity of the cellular membrane as described in [3,20], a decrease in the intracellular pH [17,19,[21][22][23][24], a mass-action effect on metabolic reactions or a direct inhibition of the activity or the synthesis of key enzymes [2,3]. CO 2 is highly soluble in cellular membrane, as revealed by a water/octanol partition coefficient of 1.3 and experimental studies on model membranes [25,26].…”

“…Furthermore, inhibition of metabolic reactions by CO 2 or bicarbonates either directly (mass-action effect) or indirectly (inhibition of the activity of the enzyme catalyzing the reaction) remains hypothetical due to the difficulty of measuring intracellular metabolites in dynamic culture conditions [28] and enzyme activity in intracellular-like environments [29]. Moreover, the impact of CO 2 on intracellular pH of microorganisms has been mentioned in numerous papers [17,19,[21][22][23][24] but has hitherto not been experimentally proved or assessed on a quantitative basis. Finally, investigation of CO 2 impact on the transcriptome of Saccharomyces cerevisiae in chemostat reveals significant changes in the transcription of genes encoding mitochondrial functions in aerobic glucose or nitrogen limited chemostats [8].…”

Innovative CO2 pretreatment for enhancing biohydrogen production from the organic fraction of municipal solid waste (OFMSW)

“…Table 4 shows that total chemicals consumption decreased significantly with a CO 2 sparging pretreatment at 30 mL/min. The total chemicals consumption was reduced by more than 20% [34,45,46]. However, with a CO 2 flowrate higher than 30 mL/min, the total chemicals consumption increased, reaching 48.0 mmol/L for a CO 2 flowrate of 500 mL/ min against a chemicals consumption of 21.9 mmol/L in control experiments i.e.…”