Effect of EMS and SA on seed germination percentage in M1 generation of Psophocarpus tetragonolobus (L.) Dc.

Abstract

In the present investigation, the two chemical mutagens namely Ethyl methanesulfonate (EMS) and Sodium azide (SA) were used to induce mutations in winged bean (Psophocarpus tetragonolobus (L.) DC.). Winged bean is a potential legume crop of the tropics with high protein and oil content in the seeds. For larger scale cultivation however, the major drawbacks possessed by winged bean should be minimized. The drawbacks such as presence of undesirable antinutritional factors, absence of market demands and labour intensive nature of crop.Hence its production and productivity need to be improved from the present level. A mutation breeding programme was undertaken with the objective of developing improved varieties of winged bean so that it can be popularized among the farmers for its cultivation over large areas. Thus in the present studies, attempts have been made to induce mutations in winged bean and to find out the effect of EMS and SA on seed germination percentage in M1 generation.

In vitro clonal propagation of nicotiana tabacum with modified medium and studying its gene alteration through bioinformatic analysis

“…Be that as it may, EMS treatment can moreover actuate undesirable impacts such as decreased practicality or sterility in a few seeds, restricting the utility of the mutagenesis approach . By and large, the impacts of EMS treatment on tobacco seeds can change depending on components such as treatment dose, length, and hereditary foundation, highlighting the significance of cautious optimization and characterization of EMS mutagenesis conventions for particular breeding or investigative purposes (Sonavane, 2016).…”

Pengaruh Ethyl Methane Sulphonate (EMS) terhadap Morfologi Akar Kecambah Padi (Oryza sativa) Varietas Lallodo

“…Hal ini didukung dari hasil uji lanjutan menggunakan uji Dunnet yang menunjukkan perlakuan EMS 1.5% dan 2% memiliki nilai signifikan >0.05 pada hari ke 1-3. Menurunnya kecepatan perkecambahan merupakan dampak dari pemberian mutagen pada proses metabolisme sel yang berdampak pada perubahan pada fisiologis sel [17]. Pada saat perkecambahan kelompok protein yang mengalami peningkatan regulasi adalah protein yang berhubungan dengan katabolisme [18].…”