Effect of enamel matrix derivative (EMDOGAIN^®) upon periodontal healing after replantation of permanent incisors in Beagle dogs

Abstract

This study histologically assessed the effect of enamel matrix derivative (EMDOGAIN) on periodontal healing in re-implanted teeth in nine Beagle dogs. Incisors in dogs were re-implanted after 15, 30 and 60 min of dry storage with and without the application of EMDOGAIN. The pulps were removed in all teeth. In groups 1 and 2, dog teeth were splinted and the dogs sacrificed after 8 and 12 weeks, respectively. In group 3, dog teeth were not splinted and sacrifice took place after 12 weeks. The histological parameters studied were healed PDL, surface, inflammatory and replacement resorption. Univariable and multivariable analysis of data was performed. Increased incidence of healed PDL was inversely proportional to extraalveolar time. No significant differences were noticed between splinted and non-splinted teeth. The EMDOGAIN group showed a higher incidence of healed PDL, while controls showed a higher incidence of ankylosis. The effect of EMDOGAIN was more pronounced at the 12-week interval.

Regulatory mechanisms of periodontal regeneration

“…In contrast, only a few investigations on the precise mechanisms have been reported with regard to the relationship between tooth implantation or replantation and regeneration of the periodontal ligament (Iqbal and Bamaas, 2001;Kawanami, et al, 2001;Lekic et al, 2001;Lin et al, 2000).…”

“…EMDOGAIN may also create an environment conducive to the formation of acellular cementum when applied to a mechanically exposed or an acid etched surface of dentin . Another study in beagle dogs has reported that the effect of EMDOGAIN upon periodontal healing is more pronounced at a 12-week interval after the replantation of permanent incisors (Iqbal and Bamaas, 2001).…”

The effect of enamel matrix protein on gingival tissue thickness in vivo

“…Among these mediators, enamel matrix protein (EMD) has proven to be efficacious in periodontal regeneration [3][4][5]. Recently, the use of EMD has been suggested in pulpotomies [6,7] and the management of avulsed permanent teeth due to its effects on the healing process [8][9][10][11].…”

Delayed Replantation of Avulsed Incisor with Prolonged Extraoral Dry Storage

“…11 Fluoride acts on the bone tissues, cementum and dentin, by converting hydroxyapatite into fluorapatite which is more resistant to resorption. Of late Iqbal and Bamaas 12 showed that extracted dogs' teeth air-dried for periods up to 60 minutes benefited from surface treatment with an enamel matrix derivative gel before replantation. Periodontal healing was greater for the treated teeth than for control teeth, and there was less replacement resorption.…”