Effect of enamel matrix derivative on human periodontal fibroblasts: proliferation, morphology and root surface colonization. An <i>in vitro</i> study

Cattaneo, Vitaliano; Rota, C.; Silvestri, Maurizio; Piacentini, C; Forlino, Antonella; Gallanti, Angelo; Rasperini, Giulio; Cetta, Giuseppe

doi:10.1034/j.1600-0765.2003.00690.x

Cited by 76 publications

(79 citation statements)

References 41 publications

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“…EMD enhances the adhesion, proliferation and matrix production of fibroblast-like but not epithelial cells (Cattaneo et al, 2003;Gestrelius et al, 1997;Haase and Bartold, 2001;Hoang et al, 2000;Kawase et al, 2000). Attachment of human periodontal ligament cells to EMD is possibly mediated by bone sialoprotein as it can be inhibited by RGD-containing peptides or with an anti-avb3 integrin antibody (Suzuki et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Critical role for αvβ6 integrin in enamel biomineralization

Mohazab

Koivisto

Jiang

et al. 2012

Journal of Cell Science

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SummaryTooth enamel has the highest degree of biomineralization of all vertebrate hard tissues. During the secretory stage of enamel formation, ameloblasts deposit an extracellular matrix that is in direct contact with the ameloblast plasma membrane. Although it is known that integrins mediate cell-matrix adhesion and regulate cell signaling in most cell types, the receptors that regulate ameloblast adhesion and matrix production are not well characterized. We hypothesized that avb6 integrin is expressed in ameloblasts where it regulates biomineralization of enamel. Human and mouse ameloblasts were found to express both b6 integrin mRNA and protein. The maxillary incisors of Itgb6 2/2 mice lacked yellow pigment and their mandibular incisors appeared chalky and rounded. Molars of Itgb6 2/2 mice showed signs of reduced mineralization and severe attrition. The mineral-to-protein ratio in the incisors was significantly reduced in Itgb6 2/2 enamel, mimicking hypomineralized amelogenesis imperfecta. Interestingly, amelogenin-rich extracellular matrix abnormally accumulated between the ameloblast layer of Itgb6 2/2 mouse incisors and the forming enamel surface, and also between ameloblasts. This accumulation was related to increased synthesis of amelogenin, rather than to reduced removal of the matrix proteins. This was confirmed in cultured ameloblast-like cells, in which avb6 integrin was not an endocytosis receptor for amelogenins, although it participated in cell adhesion on this matrix indirectly via endogenously produced matrix proteins. In summary, integrin avb6 is expressed by ameloblasts and it plays a crucial role in regulating amelogenin deposition and/or turnover and subsequent enamel biomineralization.

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Section: Discussionmentioning

confidence: 99%

Critical role for αvβ6 integrin in enamel biomineralization

Mohazab

Koivisto

Jiang

et al. 2012

Journal of Cell Science

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“…Examination of randomly selected tissues characterized the effects of treatments with GTR (5,10,19). Within the limits of our knowledge, the effects of EMP at the early stage (at 1, 2, and 4 weeks) of periodontal tissue healing have not been previously investigated in vivo.…”

Section: Discussionmentioning

confidence: 99%

Periodontal regeneration in intrabony defects after application of enamel matrix proteins with guided tissue regeneration: An experimental study in dogs

Onodera¹,

Shibukawa²,

Sugito³

et al. 2005

Biomed. Res.

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“…It has been shown that EMD has no effect on ALP activity (59); however, EMD both decreases (57) and increases (62,73,74) ALP activity in PDL fibroblasts. The production of osteopontin and osteoprotegerin has been shown to be increased by EMD (60,74).…”

Section: Emdogain and Fibroblastsmentioning

confidence: 99%

Emdogain® in carcinogenesis: a systematic review of in vitro studies

2010

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Emdogain is a commercial product of unknown composition and is clinically used to induce periodontal regeneration. This study aims to review current knowledge of the in vitro effects of Emdogain on oral tissues and, in particular, factors related to carcinoma. A systematic approach was used to review studies from the Embase and Pubmed databases; a total of 76 studies were included. These comprised in vitro studies of the cytokines in, or regulated by, Emdogain and assays designed to study the effects of EMD on human cells in oral tissues or malignant cells. Several studies have shown that EMD regulates the proliferation, migration, adhesion, gene expression, and cytokine production of (pre-)osteoblasts, periodontal fibroblasts, and gingival fibroblasts. However, the effects of EMD on malignant oral cells are not well understood. EMD seems to have broad regulatory effects on malignant cells and on several carcinoma-related factors. Evidence suggests that patients with premalignant or malignant mucosal lesions should not be treated with EMD. (J Oral Sci 52, 1-11, 2010)

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Effect of enamel matrix derivative on human periodontal fibroblasts: proliferation, morphology and root surface colonization. An in vitro study

Cited by 76 publications

References 41 publications

Critical role for αvβ6 integrin in enamel biomineralization

Critical role for αvβ6 integrin in enamel biomineralization

Periodontal regeneration in intrabony defects after application of enamel matrix proteins with guided tissue regeneration: An experimental study in dogs

Emdogain® in carcinogenesis: a systematic review of in vitro studies

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