Effect of endothelial integrity, transmural pressure, and time on the intimal-medial uptake of serum 125I-albumin and 125I-LDL in an in vitro porcine arterial organ-support system.

Arterial intimal-medial permeability and coevolving structural responses to defined shear-stress exposures

“…The gas environment was continuously monitored and refreshed by flowing fresh filtered (USP) air, O 2, and CO2 through the chamber at 37°C (29). All wells were pressurized at 100 mmHg (transmural).…”

“…It has been shown that both in vivo and ex vivo rates of transendothelial intimal EBD-alb uptake appear to be monotonically related to the corresponding rates of LDL transport (29,30). Accordingly, in the present work, the nonradioactive EBD-alb was used as a practical, easily measured, experimental "model" macromolecule for the estimation of general macromolecular mass transport processes across endothelial surfaces.…”

“…Early studies suggest that hemodynamic factors could influence the endothelial cell surface barrier in an adverse manner to initiate the chain of aberrant interstitial mass transport and cellular events of atherogenesis (2,5,23,27,30). Because one of the initiating events appears to be endothelial barrier failure (3,29,30,42,45), most subsequent work has focused on endothelial cell biology. To do this, special tissue culture systems were developed in which controlled shear stresses could be applied to cultured monolayers of endothelial cells.…”

Transport of Low-Density Lipoprotein Into the Blood Vessel Wall During Atherogenic Diet in the Isolated Rabbit Carotid Artery

“…The results further showed (Table; Figure 4) that mean values of shear stress (obtained at 0 mmHg and 140±10 mmHg) strips incubated in a tissue holding device 20 or by using incubated blood vessel segments, 21 or by using in vitro cultured endothelial cell mono-layers without changing pressure conditions. 22 Some studies presented mathematical and computational models of blood vessels based on known experimental results.…”

“…9,15- 19 The goal of this work was to examine LDL uptake by the blood vessel wall in a very short period of time, taking into account shear stress variation and possible influence of cholesterol exposure to the blood vessel wall. All previous studies that examined LDL transport and accumulation into the blood vessel wall were performed either on isolated blood vessel strips 20 or segments. 21 These were done either without perfusion flow or in vitro, using cultured endothelial cell monolayers 22 or they were performed by using mathematical and computational models of blood vessels 18,23,24 based on experimental results, especially those of Meyer et al 21 This study t is known that the leading cause of death worldwide is coronary artery disease (CAD), and special attention is given to atherosclerosis, mainly dyslipidemia, as an important risk factor for CAD.…”