Effect of Endothelial Microparticles Induced by Hypoxia on Migration and Angiogenesis of Human Umbilical Vein Endothelial Cells by Delivering MicroRNA-19b

Liang, Huizhu; Li, Sufang; Zhang, Feng; Wu, Mingxin; Li, Changlong; Song, Junxian; Lee, Chongyou; Chen, Hong

doi:10.4103/0366-6999.245271

Cited by 15 publications

(12 citation statements)

References 34 publications

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“…described to be anti-angiogenic on HUVEC cells (Liang et al, 2018). SEVs co-incubated with miR-19 were able to inhibit the formation of capillary-like structures in vitro induced by VEGF, whereas miR-19b alone did not (Figure 3F).…”

Section: Proangiogenic Effect Of Mimic Loaded Sevs In Vitromentioning

confidence: 91%

“…Loading efficacy and transfer experiments were validated using the exogenous cel-miR-39 mimic from C. Elegans . We investigate the different effects of the angiomiR-126 (miR-126) ( Tiwari et al, 2018 ) and the angiogenesis inhibitor, miR-19b ( Liang et al, 2018 ) co-incubated with SEVs both in vitro and in vivo . Moreover, the active role of proteins on EV surface on exogenous RNA binding was evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Serum Derived Extracellular Vesicles Mediated Delivery of Synthetic miRNAs in Human Endothelial Cells

Tapparo

Pomatto

Deregibus

et al. 2021

Front. Mol. Biosci.

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Extracellular vesicles (EVs) have emerged in the last decades as a cell-to-cell communication mechanism. One of their mechanism of action is the direct delivery of their cargo, composed of bioactive molecules to target cells. Different methods (direct electroporation, cell transfection, chemical transfection) were developed to vehicle therapeutic molecules through EVs. However, most of these techniques presented some limitations such as EV disruption and aggregation. In the present study, we demonstrated that a direct temperature-controlled co-incubation of EVs with defined miRNAs is a stable method to deliver information to target cells without affecting EV constitutive content. We chose serum as an easy and abundant source of EVs applicable to autologous treatment after EV modification. Exogenous cel-miR-39 loaded on serum EVs (SEVs) was taken up by human endothelial cells, demonstrating an adequate miRNA loading efficacy based on the co-incubation method. Moreover, SEVs co-incubation with the angiomiRNA-126 (miR-126) enhanced their angiogenic properties in vitro and in vivo by increasing the capacity to induce capillary-like structure formation of human endothelial cells. MiR-126 loaded EVs were also shown to stimulate mouse endothelial cells to invade Matrigel plugs and create more vessels with respect to the EV naive counterpart. When SEVs were loaded with miR-19b, an anti-angiogenic miRNA, they were able to reduce Vascular endothelial growth factors (VEGF) pro-angiogenic capacity, supporting the selective biological effect mediated by the carried miRNA. Lastly, we identified Annexin A2 (ANXA2) as one of the molecules involved in the exogenous RNA binding to serum EV surface, favoring miRNA delivery to target endothelial cells for potential therapeutic application.

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Section: Proangiogenic Effect Of Mimic Loaded Sevs In Vitromentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Serum Derived Extracellular Vesicles Mediated Delivery of Synthetic miRNAs in Human Endothelial Cells

Tapparo

Pomatto

Deregibus

et al. 2021

Front. Mol. Biosci.

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“…MiR-19b suppresses the expression of the TGFβRI and TGFβRII genes, thus downregulating the fibrogenesis process [ 22 ]. MiR-19b is regulated by the cMyc gene [ 23 ].…”

Section: Role Of Mirnas 19b-3p In Cadmentioning

confidence: 99%

Role of Selected miRNAs as Diagnostic and Prognostic Biomarkers in Cardiovascular Diseases, Including Coronary Artery Disease, Myocardial Infarction and Atherosclerosis

Mir

Elfaki

Khullar³

et al. 2021

JCDD

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Cardiovascular diseases are the leading cause of death worldwide in different cohorts. It is well known that miRNAs have a crucial role in regulating the development of cardiovascular physiology, thus impacting the pathophysiology of heart diseases. MiRNAs also have been reported to be associated with cardiac reactions, leading to myocardial infarction (MCI) and ultimately heart failure (HF). To prevent these heart diseases, proper and timely diagnosis of cardiac dysfunction is pivotal. Though there are many symptoms associated with an irregular heart condition and though there are some biomarkers available that may indicate heart disease, authentic, specific and sensitive markers are the need of the hour. In recent times, miRNAs have proven to be promising candidates in this regard. They are potent biomarkers as they can be easily detected in body fluids (blood, urine, etc.) due to their remarkable stability and presence in apoptotic bodies and exosomes. Existing studies suggest the role of miRNAs as valuable biomarkers. A single biomarker may be insufficient to diagnose coronary artery disease (CAD) or acute myocardial infarction (AMI); thus, a combination of different miRNAs may prove fruitful. Therefore, this review aims to highlight the role of circulating miRNA as diagnostic and prognostic biomarkers in cardiovascular diseases such as coronary artery disease (CAD), myocardial infarction (MI) and atherosclerosis.

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“…In contrast, high amounts of microparticles (2 × 10 5 particles/well, in a 96-well plate) had an inhibitory effect. Liang et al ( 2018 ), showed that hypoxic HUVEC EVs could also inhibit EC migration and angiogenesis probably due to increased levels of miR-19b in these EVs (5 x 10 4 EVs/well, in a 6-well plate were used). The ability of ECs to stimulate angiogenesis may involve the transfer of miR-214 (Balkom et al, 2013 ).…”

Section: Evs In Neovascularisationmentioning

confidence: 99%

Extracellular Vesicle miRNAs in the Promotion of Cardiac Neovascularisation

et al. 2020

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Cardiovascular disease (CVD) is the leading cause of mortality worldwide claiming almost 17. 9 million deaths annually. A primary cause is atherosclerosis within the coronary arteries, which restricts blood flow to the heart muscle resulting in myocardial infarction (MI) and cardiac cell death. Despite substantial progress in the management of coronary heart disease (CHD), there is still a significant number of patients developing chronic heart failure post-MI. Recent research has been focused on promoting neovascularisation post-MI with the ultimate goal being to reduce the extent of injury and improve function in the failing myocardium. Cardiac cell transplantation studies in pre-clinical models have shown improvement in cardiac function; nonetheless, poor retention of the cells has indicated a paracrine mechanism for the observed improvement. Cell communication in a paracrine manner is controlled by various mechanisms, including extracellular vesicles (EVs). EVs have emerged as novel regulators of intercellular communication, by transferring molecules able to influence molecular pathways in the recipient cell. Several studies have demonstrated the ability of EVs to stimulate angiogenesis by transferring microRNA (miRNA, miR) molecules to endothelial cells (ECs). In this review, we describe the process of neovascularisation and current developments in modulating neovascularisation in the heart using miRNAs and EV-bound miRNAs. Furthermore, we critically evaluate methods used in cell culture, EV isolation and administration.

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Effect of Endothelial Microparticles Induced by Hypoxia on Migration and Angiogenesis of Human Umbilical Vein Endothelial Cells by Delivering MicroRNA-19b

Cited by 15 publications

References 34 publications

Serum Derived Extracellular Vesicles Mediated Delivery of Synthetic miRNAs in Human Endothelial Cells

Serum Derived Extracellular Vesicles Mediated Delivery of Synthetic miRNAs in Human Endothelial Cells

Role of Selected miRNAs as Diagnostic and Prognostic Biomarkers in Cardiovascular Diseases, Including Coronary Artery Disease, Myocardial Infarction and Atherosclerosis

Extracellular Vesicle miRNAs in the Promotion of Cardiac Neovascularisation

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