Effect of endothelin-1 on osteoblastic differentiation is modified by the level of connexin43: comparative study on calvarial osteoblastic cells isolated from Cx43+/−and Cx43+/+ mice

Geneau, Graziello; Lamiche, Coralie; Niger, Corinne; Strale, Pierre‐Olivier; Clarhaut, Jonathan; Defamie, Norah; Debiais, Françoise; Mesnil, Marc; Cronier, Laurent

doi:10.1007/s00441-009-0924-5

Cited by 11 publications

(10 citation statements)

References 47 publications

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“…Proliferation and apoptotic assays Proliferation assay was performed according to Geneau et al [30]. One OD unit corresponds to 5 9 10 4 , 3.3 9 10 4 and 2.8 9 10 4 cells for OB, PC-3 and LNCaP respectively.…”

Section: Immunocytochemistrymentioning

confidence: 99%

“…To detect Cx43, procedure was modified from Geneau et al [30] using cold acetone fixation (10 min) and propidium iodide for nuclear counterstaining. Samples were observed on a confocal microscope (FV1000 Olympus IX-81, Tokyo, Japan).…”

Section: Immunocytochemistrymentioning

confidence: 99%

“…Coculture with osteoblastic (OB) cells OB cells were isolated from calvaria of 3-5 days-old mice after sequential digestions as previously described [30]. After 6 days of primary culture in DMEM with 20% FBS, OB cells were trypsinized in a 0.05% trypsin/0.15% EDTA solution, seeded at 25 9 10 3 cells/ml in a differentiating medium (DMEM supplemented with 10% FBS and 10 mM sodium b-glycerophosphate) and cultured at 37°C.…”

Section: Cell Lines and Stable Transfectionmentioning

confidence: 99%

“…As previously described [30], 50 lg of proteins were separated on 12% polyacrylamide-SDS gel and electroblotted on nitrocellulose membrane. Blots were probed overnight at 4°C with monoclonal antibody against Cx43 (1/1,000; Transduction Laboratories, Lexington, KY).…”

Section: Immunoblottingmentioning

confidence: 99%

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The gap junction protein Cx43 is involved in the bone-targeted metastatic behaviour of human prostate cancer cells

Lamiche

Clarhaut

Strale

et al. 2011

Clin Exp Metastasis

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For decades, cancer was associated with gap-junction defects. However, more recently it appeared that the gap junction proteins (connexins) could be re-expressed and participate to cancer cell dissemination during the late stages of tumor progression. Since primary tumors of prostate cancer (PCa) are known to be connexin deficient, it was interesting to verify whether their bone-targeted metastatic behaviour could be influenced by the re-expression of the connexin type (connexin43) which is originally present in prostate tissue and highly expressed in bone where it participates to the differentiation of osteoblastic cells. Thus, we investigated the effect of the increased Cx43 expression, by retroviral infection, on the metastatic behaviour of two well-characterized cell lines (PC-3 and LNCaP) representing different stages of PCa progression. It appeared that Cx43 differently behaved in those cell lines and induced different phenotypes. In LNCaP, Cx43 was functional, localized at the plasma membrane and its high expression was correlated with a more aggressive phenotype both in vitro and in vivo. In particular, those Cx43-expressing LNCaP cells exhibited a high incidence of osteolytic metastases generated by bone xenografts in mice. Interestingly, LNCaP cells were also able to decrease the proliferation of cocultured osteoblastic cells. In contrast, the increased expression of Cx43 in PC-3 cells led to an unfunctional, cytoplasmic localization of the protein and was correlated with a reduction of proliferation, adhesion and invasion of the cells. In conclusion, the localization and the functionality of Cx43 may govern the ability of PCa cells to metastasize in bones.

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Section: Immunocytochemistrymentioning

confidence: 99%

Section: Immunocytochemistrymentioning

confidence: 99%

Section: Cell Lines and Stable Transfectionmentioning

confidence: 99%

Section: Immunoblottingmentioning

confidence: 99%

See 2 more Smart Citations

The gap junction protein Cx43 is involved in the bone-targeted metastatic behaviour of human prostate cancer cells

Lamiche

Clarhaut

Strale

et al. 2011

Clin Exp Metastasis

Self Cite

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“…A growi ng num ber of li te ra tu re do cu men ts the pre sen ce and eff ec ts of en dot he li ns in bo nes (77)(78)(79)(80)(81). Bo th ETRA and ETRB ha ve been de mon stra ted in os teob las tic cel ls by li ga nd bin di ng.…”

Section: Eff E Ct Of En Dot He Lin On Bo Nesmentioning

confidence: 99%

Endothelins - clinical perspectives

et al. 2011

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En dot he li ns (ET) are a group of en do ge nous pep ti des, whi ch ha ve a stro ng and lo ng-las ti ng va so con stric ti ve eff e ct. Three iso for ms of en dot he li ns co ded by three diff e re nt ge nes ha ve been iden ti fi ed to da te. En dot he li n-1 (ET-1) is the mo st po te nt va so con stric ti ve age nt cur ren tly iden ti fi ed, and it was ori gi nal ly iso la ted and cha rac te ri zed from the cul tu re me dia of aor tic en dot he lial cel ls. Two ot her iso for ms, named en dot he li n-2 (ET-2) and en dot he li n-3 (ET-3), we re sub sequen tly iden ti fi ed, alo ng wi th struc tu ral ho mo lo gues iso la ted from the ve nom of Ac trac ta pis en gad den sis known as the sa ra fo toxi ns. The bio lo gi cal eff ec ts of en dot he lin pro duc tion are de ter mi ned via ac ti va tion of one or two G-pro tein coup led re cep to rs, en dot helin re cep to rs A (ETRA) and B (ET R B1 and ET R B2). Re cen tly en dot he lin re cep tor C (ETRC) was dis co ve red, howe ver, its fun ctio ns and dis tri bu tion sti ll re main un clear. The eff ec ts me dia ted by ET-1 via ETRA are va so con stric tion, bron cho con stric tion and sec re tion of al dos te ro ne. Ago nis ts re la ted to the ET R B1 ac ti va tion cau se va so di la ta tion by sti mu la ti ng NO, PGI2 and en dot he liu m-de ri ved hyper po la ri zi ng fac tor (EDHF). In con tra st, coup li ng to ET R B2 causes va so con stric tion. In vol ve me nt of ET has been de mon stra ted in the pat hop hysio lo gy of cer tain di sor de rs. In this re view, we dis cu ss the physio lo gi cal and pat hop hysio lo gi cal ro le of en dot he liu m-de ri ved ET-1, the phar ma co lo gy of its two re cep to rs, fo cu si ng on the ro le of ET-1 in the deve lop me nt of so me pat hop hysio lo gi cal con di tio ns. Key wor ds: en dot he lin 1; en dot he lin re cep tor; en dot he lin re cep tor an ta go ni st; en dot he lin con ver ti ng en zyme Review In tro duc tionEn dot he li ns we re fi r st iden ti fi ed in a pa per by Yanagisawa et al. as po te nt va soac ti ve pep ti des, regu la ti ng vas cu lar to ne, blood pres su re, ce ll pro li fera tion and hor mo ne pro duc tion (1). Di ver si ty in bio lo gi cal fun ction be ca me ra pid ly ap pa re nt wi th ma ny ot her ro les for the se pep ti des now des cribed (2,3). To date, three iso for ms of en dot he li ns (ET-1, ET-2 and ET-3) co ded by three diff e re nt genes ha ve been iden ti fi ed (2). The hu man ge ne for ET-1 is lo ca ted on chro mo so me 6, the ET-2 ge ne is on the fi r st chro mo so me, and ET-3 ge ne is on chromo so me 20 (2). The pla sma con cen tra tion of ET-1 in adul ts is nor mal ly be tween 0.4-8.1 pg/mL (4). Ea ch iso fo rm, howe ver, shows a tis sue type-depen de nt pat te rn of expres sion. ET-1 is wi de ly expres sed in en dot he lial cel ls, vas cu lar smoo th mus cles, cen tral ner vous system (CNS), and reproduc ti ve tis sues. Le ve ls of ET-2 are hi gh in the in testi ne and kid ney, whe reas ET-3 is main ly expres sed in the brain (5,6).En dot he lin bio synthe sis star ts fr...

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Connexins and pannexins in the skeleton: gap junctions, hemichannels and more

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2015

Cell. Mol. Life Sci.

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Regulation of bone homeostasis depends on the concerted actions of bone-forming osteoblasts and bone-resorbing osteoclasts, controlled by osteocytes, cells derived from osteoblasts surrounded by bone matrix. The control of differentiation, viability and function of bone cells relies on the presence of connexins. Connexin43 regulates the expression of genes required for osteoblast and osteoclast differentiation directly or by changing the levels of osteocytic genes, and connexin45 may oppose connexin43 actions in osteoblastic cells. Connexin37 is required for osteoclast differentiation and its deletion results in increased bone mass. Less is known on the role of connexins in cartilage, ligaments and tendons. Connexin43, connexin45, connexin32, connexin46 and connexin29 are expressed in chondrocytes, while connexin43 and connexin32 are expressed in ligaments and tendons. Similarly, although the expression of pannexin1, pannexin2 and pannexin3 has been demonstrated in bone and cartilage cells, their function in these tissues is not fully understood.

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Effect of endothelin-1 on osteoblastic differentiation is modified by the level of connexin43: comparative study on calvarial osteoblastic cells isolated from Cx43+/−and Cx43+/+ mice

Cited by 11 publications

References 47 publications

The gap junction protein Cx43 is involved in the bone-targeted metastatic behaviour of human prostate cancer cells

The gap junction protein Cx43 is involved in the bone-targeted metastatic behaviour of human prostate cancer cells

Endothelins - clinical perspectives

Connexins and pannexins in the skeleton: gap junctions, hemichannels and more

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