Effect of endothelin antagonist (TAK-044) on cerebral ischemic volume, oxidative stress markers and neurobehavioral parameters in the middle cerebral artery occlusion model of stroke in rats

Gupta, Yogendra Kumar; Briyal, Seema; Sharma, Uma; Jagannathan, Naranamangalam R.; Gulati, Anil

doi:10.1016/j.lfs.2004.11.025

Cited by 50 publications

(28 citation statements)

References 42 publications

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“…Thus, the accumulation of neutrophils and progression of the neutrophil inflammatory response after cerebral ischemia can be investigated using the ET-1 model without the confounding effects of anesthetics. However, ET-1 has also been reported to be involved in exacerbating ischemic damage, as seen by studies using ET-1 antagonists that reduce infarct formation after ischemia (Barone et al, 2000;Dawson et al, 1999;Gupta et al, 2005;Matsuo et al, 2001). Moreover, in a recent study by Lo et al (2005) in which transgenic mice overexpressing ET-1 were generated, an increase in brain edema was observed after cerebral ischemia.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory Cell Infiltration after Endothelin-1-Induced Cerebral Ischemia: Histochemical and Myeloperoxidase Correlation with Temporal Changes in Brain Injury

Weston

Jones

Jarrott

et al. 2007

J Cereb Blood Flow Metab

128

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Accumulation of neutrophils in brain after transient focal stroke remains controversial with some studies showing neutrophils to be deleterious, whereas others suggest neutrophils do not contribute to ischemic injury. Myeloperoxidase (MPO) has been used extensively as a marker for quantifying neutrophil accumulation, but is an indirect method and does not detect neutrophils alone. To elucidate the interaction of macrophages in the neutrophil inflammatory response, we conducted double-label immunofluorescence in brain sections at 0, 1, 2, 3, 7, and 15 days after ischemia. Each of these results was obtained from the same animal to determine correlations between neutrophil infiltration and ischemic damage. It was found that MPO activity increased up to 3 days after cerebral ischemia. Dual-staining revealed that macrophages engulf neutrophils in the brain and that this engulfment of neutrophils increased with time, with 50% of neutrophils in the brain engulfed at 3 days and approximately 85% at 15 days (N = 5, P < 0.05). Interestingly, at 7 days the amount of dual-staining was decreased to 20% (N = 5, P < 0.05). Neutrophil infiltration was positively correlated with ischemic damage in both the cortex and striatum (r 2 = 0.86 and 0.80, respectively, P < 0.01). The results of this study indicate that the MPO from neutrophils phagocytized by macrophages may continue to contribute to the overall MPO activity, and that previous assessments that have utilized this marker to measure neutrophil accumulation may have miscalculated the number of neutrophils within the ischemic territory and hence their contribution to the evolution of the infarct at later time points. Thus any biphasic infiltration of neutrophils may have been masked by the accumulation of macrophages.

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Section: Discussionmentioning

confidence: 99%

Inflammatory Cell Infiltration after Endothelin-1-Induced Cerebral Ischemia: Histochemical and Myeloperoxidase Correlation with Temporal Changes in Brain Injury

Weston

Jones

Jarrott

et al. 2007

J Cereb Blood Flow Metab

128

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show abstract

“…Each animal received a training session on the rota rod at constant speed of 8 rpm and was tested until it achieved the criteria of remaining on the rotating spindle for 60 s. Each rat received a single base line trial on the accelerating rota rod in which the spindle speed increased from 4 to 40 rpm over a period of 5 min. After administration of drugs for 28 days, each rat received a test trial (Gupta et al, 2005).…”

Section: Rota Rodmentioning

confidence: 99%

Safety evaluation of an Ayurvedic medicine, Arogyavardhini vati on brain, liver and kidney in rats

Kumar

Srivastava

Sharma

et al. 2012

Journal of Ethnopharmacology

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“…15,16) The critical parameters of oxidation are lipid peroxidation and its production of malondialdehyde (MDA). 17) To estimate the ability of protection against oxygen free radicals, a sensitive and reliable method called oxygen radical absorbent capacity (ORAC) assay was used. 18) Based on this knowledge, we detected the anti-stress effects of SGE on restraint-evoked immunocompromise in mice, by evaluating the effects of SGE on recovering the number of lymphocyte cells in spleen, the balance of T lymphocyte subpopulation (CD4 ϩ T/CD8 ϩ T) and NK cell activity in splenocytes of mice.…”

Section: )mentioning

confidence: 99%

The Anti-stress Effects of Sarcandra glabra Extract on Restraint-Evoked Immunocompromise

He¹,

Yao

et al. 2009

Biological & Pharmaceutical Bulletin

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Sarcandra glabra was a renowned herb traditionally used as herbal tea or food supplement to enhance mental efficiency and to recover from stress or fatigue in China. We investigated the effects of Sarcandra glabra extract (SGE), with chemical composition clearly showed by HPLC fingerprint as quality control, on immunologic response including natural killer (NK) cell activity and its antioxidative capacity in splenocytes obtained from restraint mice. Our results found that daily oral administration of SGE (125, 500 mg/kg/d) for 5 consecutive days to restrained mice alleviated the stress-induced reduction of the number of lymphocytes, the balance of CD4 ؉ T/CD8؉ T and NK cell activity per spleen. SGE also significantly decreased the level of lipid peroxidation and increased oxygen radical absorbance capacity (ORAC) in splenocytes. These results indicated that SGE modulate stress-attenuated immunologic response, at least, partially explained by improving antioxidative capacity in immunocytes.

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Effect of endothelin antagonist (TAK-044) on cerebral ischemic volume, oxidative stress markers and neurobehavioral parameters in the middle cerebral artery occlusion model of stroke in rats

Cited by 50 publications

References 42 publications

Inflammatory Cell Infiltration after Endothelin-1-Induced Cerebral Ischemia: Histochemical and Myeloperoxidase Correlation with Temporal Changes in Brain Injury

Inflammatory Cell Infiltration after Endothelin-1-Induced Cerebral Ischemia: Histochemical and Myeloperoxidase Correlation with Temporal Changes in Brain Injury

Safety evaluation of an Ayurvedic medicine, Arogyavardhini vati on brain, liver and kidney in rats

The Anti-stress Effects of Sarcandra glabra Extract on Restraint-Evoked Immunocompromise

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