Effect of endotoxin on the formation of microthrombi from circulating fibrin monomer complexes in the absence of thrombin generation

Müller-Berghaus, G.; Hocke, Michael

doi:10.1016/0049-3848(72)90034-5

Cited by 25 publications

(10 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hitherto, the fate of fibrin monomer in uiuo has not been known precisely. In a previous study purified fibrin monomer intravenously infused into rabbits was cleared from the circulation within 4-8 h (Muller-Berghaus & Heinrich, 1972). When large amounts of fibrin niononicr were intravenously administered to rabbits, microclots were formed in kidneys, lungs, liver and spleen (Muller-Berghaus et al, 1973;Lipinski ct al, 1974a).…”

mentioning

confidence: 95%

“…These soluble fibrin monomer complexes are designated as soluble fibrin. The generation of soluble fibrin in the circulating blood has been identified as an intermediate step in the activation of intravascular coagulation (Shainoff & Page, 1960, 1962 Godal & Abildgaard, 1966; Lipinski et al, 1967;Kierulf & Godal, 1971;Niewiarowski & Gurewich, 1971;Muller-Berghaus & Hocke, 1972;Graeff ct nl, 1973). Soluble fibrin was traced in small amounts even in normal rabbits (Shainoff & Page, 1960;Sherman, 1972).…”

mentioning

confidence: 99%

See 1 more Smart Citation

In Vivo Behaviour of Homologous Urea‐Soluble ¹³¹I‐Fibrin and ¹²⁵I‐Fibrinogen in Rabbits: the Effect of Fibrinolysis Inhibition

et al. 1976

Self Cite

View full text Add to dashboard Cite

The in vivo behaviour of urea-soluble fibrin monomer (FM) was compared with that of fibrinogen in rabbits. Purified rabbit 125I-fibrinogen was injected into 36 unanaesthetized rabbits. Three days later the rabbits received either purified rabbit 131I-FM I mg/kg body weight, which corresponds to cI% of the circulating plasma fibrinogen pool 131I-fibrinogen, or buffered urea only. The distribution volume of FM was 43.4 +/- 6.9 ml/kg and of fibrinogen 43.5 +/- 7.7 ml/kg (mean +/- SD). The elimination curve of urea-soluble FM as represented by the clottable 131I-radioactivity. plotted on a semi-logarithmic paper, consisted of an initial steep decay within the first 6 h and a slow flattening of the slope 6 to 24 h after injection of FM. Although a terminal single-exponential slope, as observed in fibrinogen elimination, could not be computed for the 24 h following FM injection the mean half-life time of the last segment of the clottable-radioactivity curve, between 12 h and 24 h, was 12 h. Within 24 h a mean of 83-5% of the injected FM were removed from the circulating blood. The elimination characteristics of fibrinogen were not influenced by the injected FM. Control experiments showed that buffered 3.0 M urea, the solvent of FM, does not influence distribution volume and elimination of 125I-fibrinogen. The distribution of 131I as well as 125I-radioactivities in organs representing FM and fibrinogen respectively did not differ from each other. Elevated levels of 131I-radioactivity, however, were found in the urine after FM injection suggesting an accelerated elimination of FM in comparison to fibrinogen. Fibrinolysis inhibition with high doses of aprotinin did not significantly reduce the urinary excretion of 131I-radioactivity representing breakdown of FM. Furthermore, inhibition of the fibrinolytic system had no influence on the elimination characteristics of either fibrinogen or FM. In order to explain the results obtained in this study the following theory is proposed. Intravenously injected FM forms complexes with fibrinogen and fibrinolytic degradation products. The complexes continuously increase in size until they dissociate into fibrin oligomers and carrier proteins. The oligomers are eliminated whereas the carrier proteins recycle.

show abstract

mentioning

confidence: 95%

mentioning

confidence: 99%

In Vivo Behaviour of Homologous Urea‐Soluble ¹³¹I‐Fibrin and ¹²⁵I‐Fibrinogen in Rabbits: the Effect of Fibrinolysis Inhibition

et al. 1976

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the present investigation the role of platelets in the in vivo paracoagulation phenomenon was studied. We used the model generating glomerular capillary microclots by injecting endotoxin into animals with circulating soluble fibrin previously described by Miiller-Berghaus & Hocke (1972). Since the generation of microclots by a single injection of endotoxin into rabbits with fibrinaemia cannot be prevented by heparin treatment, precipitation of soluble fibrin has been suggested.…”

Section: Discussionmentioning

confidence: 99%

“…The generation of glornerular microclots is a biphasic event. Firstly, the coagulation system has to be activated to form soluble fibrin monomer complexes (soluble fibrin), and secondly, these complexes have to be precipitated by a process independent of the coagulation mechanism (Muller- Berghaus & Hocke 1972, 1973.…”

mentioning

confidence: 99%

The Role of Blood Platelets in the Precipitation of Soluble Fibrin by Endotoxin

Eckhardt

Müller‐Berghaus

1975

Scandinavian Journal of Haematology

View full text Add to dashboard Cite

This study is intended to clarify whether or not platelets are essential to endotoxin‐induced precipitation of soluble fibrin representing the second phase of microclot formation. Renal glomerular microclots could be observed in extremely thrombocytopenic rabbits to the same extent as in normal rabbits after the injection of endotoxin into animals with circulating soluble fibrin. Thrombocytopenia was induced by feeding rabbits busulphan, and soluble fibrin was produced by infusing ancrod. The experiments indicate that platelets are not essential to the endotoxin‐induced precipitation of soluble fibrin.

show abstract

“…DesAA-fibrin may polymerize to form a clot but can also form soluble intermediates with fibrinogen (4) or fibrinolytic degradation products (5). The generation of soluble fibrin in the circulating blood has been identified as an intermediate step in the activation of intravascular coagulation (4)(5)(6)(7)(8). Soluble fibrin injected intravenously into rabbits can polymerize to microclots in kidneys, lungs, liver, and spleen, occluding the capillaries and causing necrosis of the organ tissue (9,10).…”

mentioning

confidence: 99%

Discrimination between fibrin and fibrinogen by a monoclonal antibody against a synthetic peptide.

Scheefers-Borchel¹,

Müller‐Berghaus²,

Fuhge³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Circulating soluble fibrin, observed in the blood of patients with ongoing intravascular coagulation, is generated from the plasma protein fibrinogen by the limited proteolytic action of thrombin. We report the production of a monoclonal antibody that discriminates between fibrin and fibrinogen in blood. The synthetic hexapeptide Gly-Pro-ArgVal-Val-Glu, representing the amino terminus of the a chain of human fibrin, was used as immunogen. This hexapeptide is located within the Aa chain of fibrinogen but becomes the amino terminus of the fibrin a chain, after fibrinopeptide A is removed by the action of thrombin, and thus becomes accessible for antibody binding. The monoclonal antibody we have prepared can discriminate between fibrin and fibrinogen and thus can be used in assay systems to quantitate soluble fibrin or, potentially, to image fibrin-rich thrombi.

show abstract

Effect of endotoxin on the formation of microthrombi from circulating fibrin monomer complexes in the absence of thrombin generation

Cited by 25 publications

References 10 publications

In Vivo Behaviour of Homologous Urea‐Soluble ¹³¹I‐Fibrin and ¹²⁵I‐Fibrinogen in Rabbits: the Effect of Fibrinolysis Inhibition

In Vivo Behaviour of Homologous Urea‐Soluble ¹³¹I‐Fibrin and ¹²⁵I‐Fibrinogen in Rabbits: the Effect of Fibrinolysis Inhibition

The Role of Blood Platelets in the Precipitation of Soluble Fibrin by Endotoxin

Discrimination between fibrin and fibrinogen by a monoclonal antibody against a synthetic peptide.

Contact Info

Product

Resources

About

Effect of endotoxin on the formation of microthrombi from circulating fibrin monomer complexes in the absence of thrombin generation

Cited by 25 publications

References 10 publications

In Vivo Behaviour of Homologous Urea‐Soluble 131I‐Fibrin and 125I‐Fibrinogen in Rabbits: the Effect of Fibrinolysis Inhibition

In Vivo Behaviour of Homologous Urea‐Soluble 131I‐Fibrin and 125I‐Fibrinogen in Rabbits: the Effect of Fibrinolysis Inhibition

The Role of Blood Platelets in the Precipitation of Soluble Fibrin by Endotoxin

Discrimination between fibrin and fibrinogen by a monoclonal antibody against a synthetic peptide.

Contact Info

Product

Resources

About

In Vivo Behaviour of Homologous Urea‐Soluble ¹³¹I‐Fibrin and ¹²⁵I‐Fibrinogen in Rabbits: the Effect of Fibrinolysis Inhibition

In Vivo Behaviour of Homologous Urea‐Soluble ¹³¹I‐Fibrin and ¹²⁵I‐Fibrinogen in Rabbits: the Effect of Fibrinolysis Inhibition