Effect of enteral nutrition of monoacetoacetin on bacterial translocation in burned rats

Takahata, Tetsuya; Ohira, Masaichi; Nishiguchi, Yukio; Sawai, Masamitsu; Ueda, Nobuhiko; Naito, Shinsaku; Kato, Noboru; Yukioka, Hidekazu; Hirakawa, Kosei

doi:10.1177/0148607104028005301

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…33,34 The role of bacterial translocation in generalized sepsis and multi-organ failure has been extensively studied, and its clinical relevance in burn patients has been clearly outlined. [45][46][47] Though we also searched the liver, spleen, and lung for bacteria, according to the proposed pathway implicated in multi-organ failure, 44 nothing was noted. 37 The translocation of bacteria from the intestinal lumen to the MLN has been reported in various animal models of burn injury [38][39][40] and related to constriction of mesenteric blood flow.…”

Section: Discussionmentioning

confidence: 99%

“…[42][43][44] Twenty-four hours following burn injury, we found a substantial increase in intestinal bacterial growth and translocation to MLN, but not to other organs, which was in agreement with previous studies. [45][46][47] Though we also searched the liver, spleen, and lung for bacteria, according to the proposed pathway implicated in multi-organ failure, 44 nothing was noted. Some investigators, r s = 0.499; P = .002 however, found bacteria in the spleen or the liver at 24 or 72 hours after burn, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Some investigators, r s = 0.499; P = .002 however, found bacteria in the spleen or the liver at 24 or 72 hours after burn, respectively. 46,48 Possible variation may be explained by the types of media used by the investigators and the reported types of bacteria. Our focus was to detect E coli, the virulent pathogenic bacterium responsible for septicemia and multi-organ failure, using the MacConkey Agar media.…”

Section: Discussionmentioning

confidence: 99%

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Ghrelin abates bacterial translocation following burn injury by improving gastric emptying

Sallam

Urvil

Savidge

et al. 2019

Neurogastroenterology Motil

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Background In severe burns, increased intestinal permeability facilitates bacterial translocation, resulting in systemic endotoxemia and multi‐organ failure. We investigated the role of burn‐induced gastrointestinal dysmotility (BIGD) in promoting bacterial translocation following burn injury, and the protective effect of ghrelin in this process. Methods We assessed gastric emptying (GE%) and intestinal transit (IT by geometric center “GC”) in a 60% total body surface area scald burn rat model and measured bacterial counts in mesenteric lymph nodes (MLN) and distal small intestine by colony‐forming unit per gram of tissue (CFU/g). A group of animals was treated with ghrelin or saline after burn. Key Results Scald burn was associated with a significant delay in GE (62% ± 4% vs 74% ± 4%; P = .02) and a trend of delay in intestinal transit (GC: 5.5 ± 0.1 vs 5.8 ± 0.2; P = .09). Concurrently, there was a marginal increase in small intestinal bacterial overgrowth (6 × 105 vs 2 × 105 CFU/g; P = .05) and significant translocation to MLN (2 × 102 vs 4 × 101; P = .03). We observed a negative correlation between GE and intestinal bacterial overgrowth (rs = −0.61; P = .002) and between IT and translocation (rs = −0.63; P = .004). Ghrelin administration significantly accelerated GE following burn injury (91% ± 3% vs 62% ± 4; P = .03), reduced small intestinal bacterial overgrowth, and completely inhibited translocation to MLN (0.0 vs 5 × 102; P = .01). Conclusions & Inferences Burn‐induced gastrointestinal dysmotility is correlated with the systemic translocation of gram‐negative gut bacteria that are implicated in multiple organ failure in burn patients. Therapeutic interventions to restore BIGD are warranted (Neurogastroenterol Motil, 2012, 24, 78).

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