Effect of Enterococcus faecalis EF-2001 on experimentally induced atopic eczema in mice

Choi, Eun‐Ju; Iwasa, Masahiro; Han, Kwon-Il; Kim, Wan-Jae; Tang, Yujiao; Han, Weon Cheol; Kim, Min Jung; Park, Chul–Seung

doi:10.1007/s10068-016-0175-7

Cited by 9 publications

(10 citation statements)

References 31 publications

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“…Such compounds are immunogenic and can activate macrophages, thereby contributing to the inflammatory process [ 51 ]. Human IgE antibody regulation could be affected in allergy or atopy, by Enterococcus [ 52 , 53 ]. We found elevated proportions of Enterococcus in infected persons, and increased IgE levels were associated with urogenital schistosomiasis [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

The microbiome in urogenital schistosomiasis and induced bladder pathologies

et al. 2017

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BackgroundHuman schistosomiasis is a highly prevalent neglected tropical disease (NTD) caused by Schistosoma species. Research on the molecular mechanisms influencing the outcomes of bladder infection by Schistosoma haematobium is urgently needed to develop new diagnostics, therapeutics and infection prevention strategies. The objective of the research study was to determine the microbiome features and changes in urine during urogenital schistosomiasis and induced bladder pathologies.MethodologySeventy participants from Eggua, southwestern Nigeria provided morning urine samples and were screened for urogenital schistosomiasis infection and bladder pathologies in a cross-sectional study. Highthroughput NGS sequencing was carried out, targeting the 16S V3 region. Filtered reads were processed and analyzed in a bioinformatics pipeline.Principal findingsThe study participants (36 males and 34 females, between ages 15 and 65) were categorized into four groups according to status of schistosomiasis infection and bladder pathology. Data analytics of the next-generation sequencing reads revealed that Proteobacteria and Firmicutes dominated and had influence on microbiome structure of both non-infected persons and persons with urogenital schistosomiasis. Furthermore, gender and age influenced taxa abundance independent of infection or bladder pathology. Several taxa distinguished urogenital schistosomiasis induced bladder pathologies from urogenital schistosomiasis infection alone and from healthy persons, including known immune-stimulatory taxa such as Fusobacterium, Sphingobacterium and Enterococcus. Some of these significant taxa, especially Sphingobacterium were projected as markers of infection, while several genera including potentially beneficial taxa such as Trabulsiella and Weissella, were markers of the non-infected. Finally, expected changes in protein functional categories were observed to relate to cellular maintenance and lipid metabolism.ConclusionThe urinary microbiome is a factor to be considered in developing biomarkers, diagnostic tools, and new treatment for urogenital schistosomiasis and induced bladder pathologies.

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Section: Discussionmentioning

confidence: 99%

The microbiome in urogenital schistosomiasis and induced bladder pathologies

et al. 2017

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“…The EF-2001 treatment groups were administrated orally and daily gavage doses of EF-2001 for 16 days, then colitis was induced in the three experimental groups by rectal administration of 4% DNBS in 50% ethyl alcohol for two days (Fig 1A). The dosages of EF-2001 were determined by previous reports [17,18]. All animal care procedures and experiments were approved by the Institutional Animal Care and Use Committee of Konkuk University (KU15113).…”

Section: Methodsmentioning

confidence: 99%

“…The DAI is the combined score of weight loss compared to initial weight, stool consistency, and bleeding. Scores are defined as follows: weight loss: 0 (no loss), 1 (1–5%), 2 (5–10%), 3 (10–20%), and 4 (>20%); stool consistency: 0 (normal), 2 (loose stool), and 4 (diarrhea); and bleeding: 0 (no blood), 1 (Hemoccult positive), 2 (Hemoccult positive and visual pellet bleeding), and 4 (gross bleeding, blood around anus) [18].…”

Section: Methodsmentioning

confidence: 99%

Enterococcus faecalis EF-2001 protects DNBS-induced inflammatory bowel disease in mice model

Choi

Lee

Kim³

et al. 2019

PLoS ONE

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Recent studies have demonstrated the immunomodulatory effects of heat-killed lactic acid bacteria. The aim of this study was to evaluate the protective effect of heat-killed Enterococcus faecalis EF-2001 (EF-2001) on a model of inflammatory bowel disease (IBD). A total of 28 female NC/Nga mice were divided into 4 treatment groups. Controls were fed a normal commercial diet. In the experimental groups, colitis was induced by rectal administration of dinitrobenzene sulfonic acid. Two groups were orally administered 2 and 17 mg/kg EF-2001, respectively. EF-2001 treatment decreased the expression of several cytokines, including cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), interferon (IFN)-γ, interleukin (IL)-1β, and IL-6 in inflamed colon compared to the DNBS alone group. In addition, EF-2001 suppressed DNBS-induced colonic tissue destruction. Therefore, this study strongly suggests that EF-2001 could alleviate the inflammation associated with mouse IBD.

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“…Some researchers reported that a mixture of various heatkilled L. acidophilus strains strongly released TNF-α and NO [39] and that heat-killed Enterococcus faecalis alleviated atopic symptoms in mice by reducing the IgE level, expression level of CDNB/DFE-induced inflammatory cytokines, and infiltration of mast cells [40]. Kim et al [41] showed that the viable and heat-killed LAB isolated from mukeunji showed different patterns of TNF-α and IL-6 production between species.…”

Section: Immune-stimulating Activity Of Heat-killed Lactobacillus Spementioning

confidence: 99%

Probiotic and Antioxidant Properties of Novel Lactobacillus brevis KCCM 12203P Isolated from Kimchi and Evaluation of Immune-Stimulating Activities of Its Heat-Killed Cells in RAW 264.7 Cells

Song¹,

Jang²,

Kim³

et al. 2019

Journal of Microbiology and Biotechnology

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The purpose of this study was to determine the probiotic properties of Lactobacillus brevis KCCM 12203P isolated from the Korean traditional food kimchi and to evaluate the antioxidative activity and immune-stimulating potential of its heat-killed cells to improve their bio-functional activities. Lactobacillus rhamnosus GG, which is a representative commercial probiotic, was used as a comparative sample. Regarding probiotic properties, L. brevis KCCM 12203P was resistant to 0.3% pepsin with a pH of 2.5 for 3 h and 0.3% oxgall solution for 24 h, having approximately a 99% survival rate. It also showed strong adhesion activity (6.84%) onto HT-29 cells and did not produce β-glucuronidase but produced high quantities of leucine arylamidase, valine arylamidase, β-galactosidase, and N-acetyl-βglucosaminidase. For antioxidant activity, it appeared that viable cells had higher radical scavenging activity in the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, while in the 2azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, heat-killed cells had higher antioxidant activity. Additionally, L. brevis KCCM 12203P showed higher lipid oxidation inhibition ability than L. rhamnosus GG; however, there was no significant difference (p < 0.05) between heat-killed cells and control cells. Furthermore, heat-killed L. brevis KCCM 12203P activated RAW 264.7 macrophage cells without cytotoxicity at a concentration lower than 10 8 CFU/ml and promoted higher gene expression levels of inducible nitric oxide synthase, interleukin-1β, and interleukin-6 than L. rhamnosus GG. These results suggest that novel L. brevis KCCM 12203P could be used as a probiotic or applied to functional food processing and pharmaceutical fields for immunocompromised people.

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Effect of Enterococcus faecalis EF-2001 on experimentally induced atopic eczema in mice

Cited by 9 publications

References 31 publications

The microbiome in urogenital schistosomiasis and induced bladder pathologies

The microbiome in urogenital schistosomiasis and induced bladder pathologies

Enterococcus faecalis EF-2001 protects DNBS-induced inflammatory bowel disease in mice model

Probiotic and Antioxidant Properties of Novel Lactobacillus brevis KCCM 12203P Isolated from Kimchi and Evaluation of Immune-Stimulating Activities of Its Heat-Killed Cells in RAW 264.7 Cells

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